Anti sprouty 2

Manufactured by Santa Cruz Biotechnology

Anti-Sprouty 2 is a laboratory reagent used for research purposes. It is an antibody that binds to the Sprouty 2 protein, which is involved in the regulation of cell signaling pathways. The core function of this product is to enable the detection and study of Sprouty 2 in biological samples.

Automatically generated - may contain errors

Lab products found in correlation

2 protocols using anti sprouty 2

Antibody Panel for Protein Analysis

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Antibodies used in the study were as follows: anti-Flag (F3165/F7425, Sigma), anti-Myc (9B11/71D10, Cell Signaling Technology (CST)), anti-HA (16B12, Biolegend and H6908, Sigma), anti-TRAF2 (sc-136999, Santa Cruz), anti-Rab5 (sc-46692, Santa Cruz), anti-Rab7 (sc-376362, Santa Cruz), anti-Sprouty 2 (sc-100862, Santa Cruz/ab85670, Abcam), anti-EGFR (sc-373746, Santa Cruz), anti-p-Tyr (9411, CST), anti-p-Ser/Thr (ab9344, Abcam and 05-368, Sigma), anti-Ser (sc-81514, Santa Cruz), anti-Thr (sc-5267, Santa Cruz), anti-Ubiquitin (sc-8017, Santa Cruz), anti-β-Actin (AC026, Abclonal), anti-GFP (AE011, Abclonal), anti-glutathione S-transferase (GST) (2622 S, CST), anti-V5 (R960-25, Thermo Fisher and 30801ES10, Yeasen), anti-Mouse/Rabbit IgG-peroxidase secondary antibody (A0545/A9044, Sigma), anti-Mouse IgG (light chain specific)-peroxidase secondary antibody (115-005-174, Jackson), and anti-DYRK1A polyclonal antibody has been previously described [30 (link)].

Zhang P., Zhang Z., Fu Y., Zhang Y., Washburn M.P., Florens L., Wu M., Huang C., Hou Z, & Mohan M. (2021). K63-linked ubiquitination of DYRK1A by TRAF2 alleviates Sprouty 2-mediated degradation of EGFR. Cell Death & Disease, 12(6), 608.

+ Open protocol

+ Expand

Western Blotting of Key Signaling Proteins

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Western blotting was carried out as described previously [37 (link)]. The following primary antibodies were used for western blotting: anti-MED23 (BD, 550492), anti-MED16 (Bethyl, A303-668A, RRID: AB_11205632), anti-MED24 (Bethyl, A301-472A, RRID: AB_999675), anti-phosphorylated-Erk1/2 (Thr202/Tyr204) (Santa Cruz, sc-16982), anti-Erk (K-23) (Santa Cruz Biotechnology, sc-94-G, RRID: AB_631456), anti-phosphorylated-Akt (S473) (Cell signaling, 9271), anti-Akt (Cell signaling Technology, 9272, RRID: AB_329827), anti-Ets1 (Santa Cruz, sc-350x), anti-Sprouty2 (Santa Cruz, sc-30049), anti-Src (Cell signaling Technology, 2108s, RRID: AB_331137) and β-Actin (Sigma, A5441).

Fu X., Liu S., Cao D., Li C., Ji H, & Wang G. (2024). Med23 deficiency reprograms the tumor microenvironment to promote lung tumorigenesis. British Journal of Cancer, 130(5), 716-727.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!