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Gel electrophoresis buffer

Manufactured by Bio Basic
Sourced in Canada

Gel electrophoresis buffer is a solution used in the process of gel electrophoresis, a technique used to separate and analyze DNA, RNA, or protein molecules based on their size and charge. The buffer provides the necessary ionic environment for the effective separation and migration of the biomolecules through the gel matrix.

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2 protocols using gel electrophoresis buffer

1

DNA Extraction and Agarose Gel Electrophoresis

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DNA primers were designed and obtained from Integrated DNA Technologies (Coralville, IA, USA). A Wizard genomic DNA extraction kit, 100-base pair (bp) ladder, and PCR master mix were purchased from Promega (Madison, WI, USA). Agarose gel and gel electrophoresis buffer (10Ă— Tris-EDTA-borate buffer) were purchased from BioBasic (Markham, ON, Canada). RedSafe dye was purchased from iNtRON Biotechnology (Seongnam, South Korea).
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2

Comprehensive Streptozotocin-Induced Diabetes Protocol

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Streptozocin, citric acid, sodium citrate, isopropyl alcohol, and 75% ethanol were obtained from Sigma-Aldrich (St. Louis, MO, USA). Human insulin Mixtard®, which contained 30% of soluble insulin and 70% of isophane insulin, was purchased from Novo Nordisk (Bagsvaerd, Denmark). The Trizol solution and cDNA synthesis kit were bought from Thermo Fisher Scientific (Waltham, MA, USA). The polymerase chain reaction (PCR) primers were bought from Integrated DNA technologies (Coralville, IA, USA). The Taq polymerase buffer, deoxynucleotide triphosphates (dNTPs), nuclease-free water, DNA ladder, and MgCl2 were earned from (Promega, Madison, WI, USA). Agarose gel and gel electrophoresis buffer were bought from (Bio-basic, Markham, ON, Canada). Finally, the Redsafe dye was bought from (iNtRON Biotechnology, Seoul, South Korea).
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