E. coli strains TOP10, BL21(DE3) and BL21(DE3)pLysS, and pET-28a and pET-32a plasmids were obtained from Novagen (Madison, WI). KpnI, XhoI, and NdeI restriction enzymes, T4 DNA Ligase, GeneJet Gel Extraction kit, and GenJet plasmid Preparation kit were obtained from Thermo scientific (MD, USA). Luria-Bertani (LB) medium was purchased from HiMedia (Mumbai, Maharashtra, India). Ni-NTA resin was provided by GenScript (Piscataway, USA). All recombinant DNA manipulation procedures were performed according to standard molecular biology techniques.[19 ]
Genjet plasmid preparation kit
Manufactured by Thermo Fisher Scientific
Sourced in United States, Lithuania
The GenJet plasmid Preparation kit is a laboratory product designed for the purification and extraction of plasmid DNA from bacterial cultures. It provides a simple and efficient method for obtaining high-quality plasmid DNA samples.
Automatically generated - may contain errors
Lab products found in correlation
T4 dna ligase, by Thermo Fisher Scientific (2 mentions)
Genejet gel extraction kit, by Thermo Fisher Scientific (1 mentions)
Ni nta resin, by GenScript (1 mentions)
Luria bertani medium, by HiMedia (1 mentions)
Kanamycin, by Merck Group (1 mentions)
Genjet gel extraction kit, by Thermo Fisher Scientific (1 mentions)
Fastdigest restriction endonucleases, by Thermo Fisher Scientific (1 mentions)
Isopropyl β d 1 thiogalactopyranoside (iptg), by Merck Group (1 mentions)
2 protocols using genjet plasmid preparation kit
1
Recombinant Protein Expression in E. coli
2
Recombinant Protein Expression in E. coli
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Top 10 and BL21 (DE3) Escherichia coli (E. coli) strains, pET28a expression plasmid, MCF-7 (human breast carcinoma cell line), and HUVEC (human umbilical vein endothelial cell) from Pasteur Institute of Iran (Tehran, I.R Iran); Kanamycin and isopropyl β-D-1-thiogalactopyranoside from Sigma (San Diego, California, USA). Fast Digest™ restriction endonucleases, GenJET plasmid preparation kit, and GenJET gel extraction kit from Thermoscientific (Fermentas; Vilnius, Lithuania). Other materials in the molecular biology grade were in commercial form. All tests performed according to the Sambrook and Russell protocols unless otherwise mentioned (16 ).
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