Hyclone water

Manufactured by GE Healthcare

Sourced in United States

HyClone water is a high-purity water product designed for use in laboratory and research applications. It is produced through a purification process that ensures consistent quality and low levels of impurities. The core function of HyClone water is to provide a reliable source of clean, filtered water for various laboratory procedures and experiments.

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Lab products found in correlation

Peptone water, by Merck Group (2 mentions) Foetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions) Milli q purification system, by Merck Group (2 mentions) Illustra templiphi 100 amplification kit, by GE Healthcare (2 mentions) Phusion u hot start dna polymerase, by Thermo Fisher Scientific (2 mentions) Gblock gene fragment, by Integrated DNA Technologies (2 mentions) Mach1, by Thermo Fisher Scientific (2 mentions) Mueller hinton broth (mhb), by Avantor (2 mentions) Alginate, by Merck Group (1 mentions) Sodium alginate, by Merck Group (1 mentions) Gelatin, by Merck Group (1 mentions) Gelatin type b, by Merck Group (1 mentions) Em 910, by Zeiss (1 mentions) Dnase 1, by Thermo Fisher Scientific (1 mentions) Trypsin, by Merck Group (1 mentions)

6 protocols using hyclone water

Synthetic Wound Fluid for Research

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Synthetic wound fluid (SWF) comprised equal volumes of foetal bovine serum (Gibco) and peptone water (Sigma-Aldrich)^{24 (link)}. Mueller–Hinton Broth (MHB) was obtained from VWR. HyClone water (GE Healthcare) was used throughout.

Furner-Pardoe J., Anonye B.O., Cain R., Moat J., Ortori C.A., Lee C., Barrett D.A., Corre C, & Harrison F. (2020). Anti-biofilm efficacy of a medieval treatment for bacterial infection requires the combination of multiple ingredients. Scientific Reports, 10, 12687.

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Alginate-Gelatin Hydrogel Bioprinting

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0.3 g of gelatin (Type B, Sigma-Aldrich, St. Louis, MO, USA) is first dissolved in 10 ml of water (HyClone™ Water, GE Healthcare Life Sciences). The covered glass beaker is maintained at ~40°C while being magnetically stirred. On gelatin dissolution, 0.3 g of sodium alginate (Sigma-Aldrich, St. Louis, MO, USA) is added to the solution and mixed for at least 6 h to prepare the gelatin-alginate hydrogel. 8 × 10⁶ cells are suspended in 200 µL of CCM and mixed with 4 ml of hydrogel for 5 min in the beaker using a magnetic stirrer to obtain a uniform distribution of cells (2 × 10⁶ cells/ml) in the gelatin-alginate bioink. The bioink is then transferred to a 3 ml syringe barrel attached with 22G or 410 µm internal diameter tips before bioprinting. To prepare a bioink with the incorporation of glass, 0.06 g of 13-93B3 glass (10 wt% of hydrogel material) is added to the gelatin solution and stirred to obtain uniform suspension of glass particles. sodium alginate is then added to the solution and stirred to obtain the Alg-Gel-glass hydrogel. ASCs suspended in CCM are first hand-mixed in gel and later magnetically stirred for no more than 5 min to obtain a uniform distribution of the ASCs in the bioink.

Kolan K.C., Semon J.A., Bromet B., Day D.E, & Leu M.C. (2019). Bioprinting with human stem cell-laden alginate-gelatin bioink and bioactive glass for tissue engineering. International Journal of Bioprinting, 5(2.2), 204.

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Synthesis and Characterization of Optimized DNA Nanoparticles

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Endotoxin free codon-optimized pPEI-MOP-co-sgRho, pshRho-1, pshRho-2 and psh-Rho-3 were amplified by Aldevron (Fargo, ND, USA) and separately mixed with TAT-CK30PEG-Acetate (at N: P=1:2 ratio) following the earlier protocol [11 (link),20 (link),22 (link),30 (link),33 ]. All of the DNA NPs were prepared and stored in saline (Sigma, Cat. No. S8776). The DNA NPs synthesis was confirmed by an agarose gel retardation assay. The intactness of DNA after compaction with diblock polymer was evaluated using a trypsin (Sigma-Aldrich, Cat. No. T4174) digestion assay. Protection of DNA from nuclease was evaluated by a DNAse I (Invitrogen) digestion assay. The DNA NPs were characterized by high-resolution transmission electron microscopy (HR-TEM, Zeiss EM 910), dynamic light scattering (DLS) and zeta potential (Nano-ZS zeta sizer, Malvern, USA) experiments. The entire synthesis process was carried out using endotoxin-free water (HyClone™ Water, GE healthcare) and endotoxin-free systems.

Mitra R.N., Zheng M., Weiss E.R, & Han Z. (2017). Genomic Form of Rhodopsin DNA Nanoparticles Rescued Autosomal Dominant Retinitis Pigmentosa in the P23H Knock-in Mouse Model. Biomaterials, 157, 26-39.

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Antibiotics usage and DNA manipulation protocols

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Antibiotics (Gold Biotechnology) were used at the following working concentrations: carbenicillin 50 μg/mL, spectinomycin 50 μg/mL, chloramphenicol 25 μg/mL, kanamycin 50 μg/mL, tetracycline 10 μg/mL, streptomycin 50 μg/mL. HyClone water (GE Healthcare Life Sciences) was used for PCR reactions and cloning. For all other experiments, water was purified using a MilliQ purification system (Millipore). Phusion U Hot Start DNA polymerase (Thermo Fisher Scientific) was used for all PCRs. Plasmids and SPs were cloned by USER assembly^{21 (link)}. Genes were obtained as synthesized gBlock gene fragments from Integrated DNA Technologies or PCR amplified directly from E. coli genomic DNA. Plasmids were cloned and amplified using either Mach1 (Thermo Fisher Scientific) or Turbo (New England BioLabs) cells. Unless otherwise noted, plasmid or SP DNA was amplified using the Illustra Templiphi 100 Amplification Kit (GE Healthcare Life Sciences) prior to Sanger sequencing. A full list of plasmids used in this work is given in Supplementary Table 5. A full list of reagents and equipment used in this work is given in Supplementary Table 6.

Wang T., Badran A.H., Huang T.P, & Liu D.R. (2018). Continuous directed evolution of proteins with improved soluble expression. Nature chemical biology, 14(10), 972-980.

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Antibiotics usage and DNA manipulation protocols

Cited 1 time

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Wang T., Badran A.H., Huang T.P, & Liu D.R. (2018). Continuous directed evolution of proteins with improved soluble expression. Nature chemical biology, 14(10), 972-980.

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Synthetic Wound Fluid Preparation

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Synthetic wound fluid (SWF) comprised equal volumes of foetal bovine serum (Gibco) and peptone water (Sigma-Aldrich), (23) . Mueller-Hinton Broth (MHB) was obtained from VWR.
HyClone water (GE Healthcare) was used throughout.

Furner-Pardoe J., Anonye B.O., Cain R., Moat J., Ortori C.A., Lee C., Barrett D.A., Corre C., & Harrison F. (2020). Anti-biofilm efficacy of a medieval treatment for bacterial infection requires the combination of multiple ingredients.

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