The morphology of the nanoparticles was studied using the H-7650 transmission electron microscope, manufactured by Hitachi High-Technologies Corporation in Pleasanton, CA, USA. A single drop of the CS Nanoparticle dispersion was placed on carbon 400 mesh copper grid (Ted Pella, Redding, CA, USA). The copper grids were then dried overnight in a sterile fume hood and the images were taken using the Quartz PCI version 8 software (Quartz Imaging Company, Vancouver, BC, Canada) in TEM mode (200 kV).
Carbon 400 mesh copper grid
Manufactured by Ted Pella
Sourced in United States
The Carbon 400 mesh copper grid is a laboratory equipment used for specimen support in transmission electron microscopy (TEM) applications. It provides a thin, conductive, and sturdy platform to hold samples for high-resolution imaging and analysis.
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Lab products found in correlation
2 protocols using carbon 400 mesh copper grid
1
Nanoparticle Morphology Characterization
2
Cryo-TEM Imaging of CNC-g-POx Dispersions
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TEM images were
recorded in low-dose conditions using a TALOS F200C (Thermo Fischer
Scientific) microscope working at 80 kV equipped with a Ceta 16 M
4 k × 4 k pixel CMOS camera (Thermo Fischer Scientific). TEM
samples were prepared by the deposition of CNC-g-POx
dispersions (0.01 wt %) on a carbon 400 mesh copper grid (Ted Pella
Inc.) and removing the excess after 1 min, followed by staining with
a 1% uranyl acetate solution for 30 s.
Cryo-TEM images were
recorded in low-dose conditions, with a defocus range of 1–3
μm, using a TALOS F200C (Thermo Fischer Scientific) microscope
working at 80 kV equipped with a Ceta 16 M 4 k × 4 k pixel CMOS
camera (Thermo Fischer Scientific). Samples were prepared by deposition
of CNC-g-POx dispersions (0.1 wt %) onto a Lacey
Carbon 300 mesh copper grid (Ted Pella Inc.) in a controlled environment
vitrification system (Vitrobot Mark IV, Thermo Fischer Scientific—formerly
FEI) at controlled temperature (22 °C) in 100% humidity, blot
force of 5 and blot time of 2.5 s.
For both sample preparations,
grids were previously subjected to
a glow discharge treatment using an easiGlow discharge system (Pelco)
with 15 mA negative current for 10 s in air atmosphere to make them
hydrophilic. Sample preparation and data acquisition were performed
at the Electron Microscopy Laboratory/Brazilian Nanotechnology National
Laboratory (LNNano).
recorded in low-dose conditions using a TALOS F200C (Thermo Fischer
Scientific) microscope working at 80 kV equipped with a Ceta 16 M
4 k × 4 k pixel CMOS camera (Thermo Fischer Scientific). TEM
samples were prepared by the deposition of CNC-g-POx
dispersions (0.01 wt %) on a carbon 400 mesh copper grid (Ted Pella
Inc.) and removing the excess after 1 min, followed by staining with
a 1% uranyl acetate solution for 30 s.
Cryo-TEM images were
recorded in low-dose conditions, with a defocus range of 1–3
μm, using a TALOS F200C (Thermo Fischer Scientific) microscope
working at 80 kV equipped with a Ceta 16 M 4 k × 4 k pixel CMOS
camera (Thermo Fischer Scientific). Samples were prepared by deposition
of CNC-g-POx dispersions (0.1 wt %) onto a Lacey
Carbon 300 mesh copper grid (Ted Pella Inc.) in a controlled environment
vitrification system (Vitrobot Mark IV, Thermo Fischer Scientific—formerly
FEI) at controlled temperature (22 °C) in 100% humidity, blot
force of 5 and blot time of 2.5 s.
For both sample preparations,
grids were previously subjected to
a glow discharge treatment using an easiGlow discharge system (Pelco)
with 15 mA negative current for 10 s in air atmosphere to make them
hydrophilic. Sample preparation and data acquisition were performed
at the Electron Microscopy Laboratory/Brazilian Nanotechnology National
Laboratory (LNNano).
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