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Ckitw sh mice

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CKitw-sh mice are a strain of mice that carry a mutation in the c-Kit gene, resulting in a white spotting phenotype. These mice are a model used for research purposes.

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Lab products found in correlation

C57bl 6 mice, by Jackson ImmunoResearch (1 mentions) Balb c mice, by Jackson ImmunoResearch (1 mentions) B6 sjl ptprcapepcb boyj b6 cd45.1, by Jackson ImmunoResearch (1 mentions) C57 b6j mice, by Jackson ImmunoResearch (1 mentions) Wistar rats, by Jackson ImmunoResearch (1 mentions) C57bl 6j, by Jackson ImmunoResearch (1 mentions) Wild type c57bl 6, by Jackson ImmunoResearch (1 mentions) Microtainer edta tubes, by BD (1 mentions)

Close spelling variants of this product

Sash(cKitW-Sh) mice

6 protocols using ckitw sh mice

1

Mast Cell-Deficient Mouse Model

Cited 1 time
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Six-to 8-week-old (young) and 8-to-12 month-old (aged) C57BL/6 wild-type and age-matched mast cell-deficient cKitw-sh mice, congenic to C57BL/6 mice, (Stock No: 012861) were purchased from the Jackson Laboratory (Bar Harbor, ME) for the described experiments. cKitw-sh mice were confirmed for their deficiency in mast cells at the ocular surface and the lacrimal gland using flow cytometry. cKitw-sh mice, unlike the other mast cell-deficient strain (cKitw-v), have a comparable generation of CD45+ immune cells in the bone marrow9 (link),43 (link). The mice were housed in the Schepens Eye Research Institute animal vivarium. All experiments were reviewed and approved by the Schepens Eye Research Institute Animal Care and Use Committee. The mice were treated according to the ARVO Statement for the Use of Animals in Ophthalmic and Vision Research.
Elbasiony E., Cho W.J., Singh A., Mittal S.K., Zoukhri D, & Chauhan S.K. (2023). Increased activity of lacrimal gland mast cells are associated with corneal epitheliopathy in aged mice. NPJ Aging, 9(1), 2.
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2

Mast Cell Deficiency in Mice

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Six- to eight-week-old BALB/c and C57BL/6 wild-type and mast cell-deficient cKitw-sh mice (Stock No: 012861) were purchased from Jackson Laboratory, Bar Harbor, ME for the described experiments. The mice were housed in the Schepens Eye Research Institute animal vivarium and treated according to the Use of Animals in Ophthalmic and Vision Research guidelines set forth by the Association for Research in Vision and Ophthalmology. All protocols were approved by the Animal Care and Use Committee of Schepens Eye Research Institute.
Cho W., Mittal S.K., Elbasiony E, & Chauhan S.K. (2020). Activation of Ocular Surface Mast Cells Promotes Corneal Neovascularization. The ocular surface, 18(4), 857-864.
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3

Diverse Mouse and Rat Models for Immunology

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Mouse and rat experiments were performed at MIT or the La Jolla Institute for Immunology (LJI). All experimental procedures were approved by institutional IACUC committees. Experiments were done using sex- and age-matched female mice between 6-12 weeks of age. Balb/C mice (strain: 000671) and C57B/6J mice (strain: 000664) were purchased form the Jackson Laboratory. VRC01gHL mice, BCR transgenic knock-in mice carrying inferred germline reverted VRC01 IgH and IgL (34 (link)), were maintained on the B6.GFP (strain 004353; Jackson Laboratory), C57BL/6J or B6.SJL-Ptprcapepcb/BoyJ (B6.CD45.1) (strain 002014; Jackson Laboratory) backgrounds as homozygous lines at LJI. SMARTA mice were maintained on the B6.CD45.1 background at LJI. Mast cell deficient c-Kitw-sh mice (strain 030764; Jackson Laboratory) were used for lymphatic drainage mechanism studies. Female 12-16 wk old Wistar rats (the Jackson Laboratory) were used for imaging antigen drainage from intradermal injections.
Silva M., Kato Y., Melo M.B., Phung I., Freeman B.L., Li Z., Roh K., Van Wijnbergen J.W., Watkins H., Enemuo C.A., Hartwell B.L., Chang J.Y., Xiao S., Rodrigues K.A., Cirelli K.M., Li N., Haupt S., Aung A., Cossette B., Abraham W., Kataria S., Bastidas R., Bhiman J., Linde C., Bloom N.I., Groschel B., Georgeson E., Phelps N., Thomas A., Bals J., Carnathan D.G., Lingwood D., Burton D.R., Alter G., Padera T.P., Belcher A.M., Schief W.R., Silvestri G., Ruprecht R.M., Crotty S, & Irvine D.J. (2021). A particulate saponin/TLR agonist vaccine adjuvant alters lymph flow and modulates adaptive immunity. Science immunology, 6(66), eabf1152.
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4

Mast Cell Deficiency in C57BL/6 Mice

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Six- to eight-week-old male and female C57BL/6 wild-type and mast cell deficient cKitw-sh mice (Stock No: 012861; Jackson Laboratory, Bar Harbor, ME) were purchased for use in the described experiments. The mice were housed in the Schepens Eye Research Institute animal vivarium. All experiments were reviewed and approved by the Schepens Eye Research Institute Animal Care and Use Committee. The mice were treated according to the ARVO Statement for the Use of Animals in Ophthalmic and Vision Research.
Elbasiony E., Mittal S.K., Foulsham W., Cho W, & Chauhan S.K. (2020). Epithelium-derived IL-33 activates mast cells to initiate neutrophil recruitment following corneal injury.. The ocular surface, 18(4), 633-640.
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5

Mast Cell-Deficient Mice Model

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All animal procedures followed institutional guidelines and were approved by the Institutional Animal Care and Use Committees (IACUC) of Michigan State University (NIH Assurance D16-0054). All procedures followed the recommendations in the ARRIVE guidelines. Male C57Bl/6 mice and male mast cell-deficient (C-kitW-sh) mice (9–12 weeks old; Jackson Laboratory, Bar Harbor, ME USA) were group housed in a temperature- and humidity-controlled environment with a 12-h light/dark cycle and ad libitum access to standard chow and water. Mice were euthanized by intraperitoneal injection of pentobarbital (> 150 mg/kg) followed by decapitation prior to all experimental procedures.
Saxena P., Broemer E., Herrera G.M., Mingin G.C., Roccabianca S, & Tykocki N.R. (2023). Compound 48/80 increases murine bladder wall compliance independent of mast cells. Scientific Reports, 13, 625.
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6

Hematological Profiling of C-Kit w-sh Mice

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Animals: Animals were housed in the AAALAC accredited facilities of the CWRU School of Medicine. Husbandry and experimental procedures were approved by the Case Western Reserve University Institutional Animal Care and Use Committee (IACUC) in accordance with approved IACUC protocols 2019-0065. C-Kit w-sh mice were purchased from The Jackson Laboratory. Littermate wild-type control animals were used as comparator animals for all studies and designated "LC", and a combination of male and female mice, aged 8-12 weeks old, were used for all studies. Mice were housed in standard microisolator cages and maintained on a (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. defined, irradiated diet and autoclaved water. All animals were observed daily for signs of illness.
Complete Blood Count Analysis: Peripheral blood was collected into Microtainer EDTA tubes (Becton-Dickinson) by submandibular cheek puncture. Blood counts were analyzed using a Hemavet 950 FS hematology analyzer.
Smith J., Campanelli J., Cordova B., Broncano A.V., Christo K.F., Gerson S.L., Markowitz S.D., & Desai A.B. (2022). Inactivating Mast Cell Function Promotes Steady-State and Regenerative Hematopoiesis.
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