Ten mice chosen at random from every group had their estrous cycle checked daily and body weights monitored every 4 days during the experimental period. Estrous cycle staging was done by vaginal smear cytological examination every morning between 9 a.m. and 10 a.m. Smears were obtained by flushing 20 µl of sterile NS into the vaginal cavity, spreading the flushed fluid on a glass slide, and fixing the samples with 95% ethanol for 30 min. The Wright-Giemsa Stain solution (Solarbio Science & Technology Co., Ltd., Beijing, China) was used to stain the slides, which were then rinsed with running water, air-dried, and then observed using a light microscope at ×100 magnification.
Wright giemsa stain solution
Manufactured by Solarbio
Sourced in China
The Wright-Giemsa Stain solution is a ready-to-use staining solution designed for the differential staining of blood smears and other cytological preparations. It is a combination of the Wright stain and the Giemsa stain, which provide a clear and distinct visualization of cellular elements such as nuclei, cytoplasm, and intracellular structures.
Automatically generated - may contain errors
Lab products found in correlation
Dexamethasone d4902, by Merck Group (1 mentions)
Radio immunoprecipitation assay ripa lysis buffer, by Beyotime (1 mentions)
Annexin 5 fitc pi cell apoptosis detection kit, by Transgene (1 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
N acetyl cysteine nac, by Yuanye Bio-Technology (1 mentions)
Eclipse 80i, by Nikon (1 mentions)
Inverted fluorescence microscope, by Zeiss (1 mentions)
8 protocols using wright giemsa stain solution
1
Estrous Cycle and Body Weight Monitoring
2
Dexamethasone and LPS-induced Inflammation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Dexamethasone (D4902) and LPS (L2880, from E. coli 055:B5) were purchased from Sigma-Aldrich (St. Louis, MO, USA). N-acetylcysteine (A9165-25G) was obtained from Thermo Fisher Scientific Inc. (Waltham, MA, USA). Chloral hydrate (R24095) was acquired from Shanghai yuanye Bio-Technology Co., Ltd. (Shanghai, China). Wright–Giemsa Stain solution was purchased from Beijing Solarbio Science & Technology Co., Ltd. (Beijing, China). The other reagents used were of analytical grade.
3
Bronchoalveolar Lavage Fluid Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
After being euthanized with 2% pentobarbital sodium (150 mg/kg), the left lung was ligated and the right lung was lavaged with 0.8 mL cold DPBS three times through a catheter. After being centrifuged (1500 rpm; 4°C) for 10 minutes, the supernatant of BALF was stored at −80°C for cytokines detection, and the cell deposits were resuspended with 500 μL DPBS for cell counting and BALF smear. Following the manufacturer’s protocol, BALF smears were stained with Wright-Giemsa stain solution, which is purchased from Beijing Solarbio Science & Technology Co., Ltd. (Beijing, China), to differentiate eosinophils and neutrophils. Total cells and differentiated cells were counted using ImageJ software (version 1.8.0; National Institutes of Health).
4
Optimizing Antioxidant and Anticancer Effects
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
GA (purity ≥98%), PTX (purity ≥98%), and N-acetyl cysteine (NAC; purity ≥98%) were purchased from Shanghai Yuanye Bio-Technology Co., Ltd. An MTT reagent kit and dimethyl sulfoxide (DMSO) were purchased from MilliporeSigma. Wright-Giemsa stain solution, and haematoxylin and eosin (H&E) Staining Kit were purchased from Beijing Solarbio Science & Technology Co., Ltd. Propidium iodide (PI), RNase-A, Triton X-100, ROS DCFH-DA assay kit (cat. no. S0033S), and radioimmunoprecipitation assay (RIPA) lysis buffer were purchased from Beyotime Institute of Biotechnology. An Annexin V-FITC/PI cell apoptosis detection kit was purchased from Beijing TransGen Biotech Co., Ltd. Dihydroethidium (DHE; cat. no. KGAF019) assay kit was purchased from Nanjing KeyGen Biotech Co., Ltd. Antibodies against epidermal growth factor receptor (EGFR; cat. no. 18986-1-AP), PI3K (cat. no. 20584-1-AP), Akt (cat. no. 10176-2-AP), phosphorylated (p)-Akt (cat. no. 66444-1-Ig), β-actin (cat. no. 20536-1-AP), cyclin-dependent kinase 4 (CDK4; cat. no. 11026-1-AP), cyclin B1 (cat. no. 28603-1-AP), GAPDH (cat. no. 10494-1-AP), matrix metallopeptidase 2 (MMP-2; cat. no. 10373-2-AP), Nrf2 (cat. no. 16396-1-AP), Keap-1 (cat. no. 10503-2-AP), NQO1 (cat. no. 11451-1-AP), and Ki-67 (cat. no. 27309-1-AP) were purchased from Wuhan Sanying Biotechnology.
5
ILC2 Cell Morphology Staining
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The sorted ILC2 cell suspension was evenly smeared on a glass slide and stained with Wright-Giemsa stain solution (Solarbio, Beijing, China). After 1–2 min, we added an equal amount of 0.01 M disodium hydrogen phosphate solution to the smear, mixed it with the Wright-Giemsa stain solution thoroughly, stained it for 3–5 min and then flushed it with water. The morphology of ILC2was observed under light microscope.
6
Giemsa Staining of Third-Generation DPCs
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The third generation of DPCs was plated on cover glass (WHB, China) in 6-well plates for culturing 3 days. Removed the basal medium and rinsed for three times using phosphate buffer solution (PBS). Added 2–3 drops of Wright-Giemsa Stain solution (Solarbio, #G1020, China) for rinsing 2 min. And then washed with water, dried with the hydro-paper and observed the cell morphology by using a fluorescence microscope (Nikon ECLIPSE 80i, Japan).
7
Murine Estrous Cycle Identification
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The estrous cycle stage of female mice was identified by vaginal cytology. Cells were collected with a cotton swab moistened with saline solution, using a rolling motion against the vaginal epithelial wall, and transferred onto a slide. Slides were air-dried and stained with Wright-Giemsa Stain solution (G1020, Solarbio). Slides were rinsed with double distilled water (ddH2O) and air-dried before four estrous stages assessment under a bright field microscope.
8
Evaluating Anti-Toxoplasma Therapy In Vitro
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
RPE cells were seeded in 24-well plate until a cell monolayer formed, and RH-GFP tachyzoites were added to each well at a ratio of 10:1 to cells. After 4 h of infection, drug-containing medium (750, 500, 250 nM) was replaced to each well and continued to maintain for 48 h. The proliferation of tachyzoites was observed by randomly selecting fields under the inverted fluorescence microscope (ZEISS, Germany). In order to better observe the intracellular proliferation of parasite, the cells were infected with RH tachyzoites and treated with the drug (750, 375 nM) for 48 h. Staining according to Wright-Giemsa Stain solution instruction (Solarbio, Beijing, China), random fields of view were selected under light microscope for observation. The image data obtained in the experiment were analyzed with image-J.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!