Goat anti rat hrp

Manufactured by Abcam

Sourced in Macao

Goat anti-rat HRP is a secondary antibody conjugated with horseradish peroxidase (HRP). It is used to detect and visualize target proteins in various immunological techniques, such as Western blotting, ELISA, and immunohistochemistry, where rat primary antibodies are employed.

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Lab products found in correlation

Goat anti mouse hrp, by GE Healthcare (2 mentions) Mouse monoclonal anti gfp, by Roche (2 mentions) Rabbit anti g6pdh, by Merck Group (2 mentions) Goat anti rabbit hrp, by GE Healthcare (2 mentions) Mouse anti ha, by Fortrea (2 mentions) Rat anti ha, by Roche (2 mentions) 2 nitrophenyl β d galactopyranoside, by Merck Group (1 mentions) Glass bead, by Biospec (1 mentions) Precast polyacrylamide gel, by Thermo Fisher Scientific (1 mentions) Anti alpha tubulin, by Bio-Rad (1 mentions) Goat anti mouse hrp, by Abcam (1 mentions) Anti flag m2, by Merck Group (1 mentions) Pvdf membrane, by Bio-Rad (1 mentions) Trans blot turbo transfer system, by Bio-Rad (1 mentions)

Close spelling variants of this product

Goat Anti-Rat IgG H&L HRP HRP-conjugated goat anti-rat IgG HRP)-conjugated goat anti-rat secondary antibody Goat-anti-rat-HRP conjugate Anti-rat HRP

3 protocols using goat anti rat hrp

Yeast Strain and Plasmid Characterization

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Yeast (Saccharomyces cerevisiae) strains used in this paper are summarized in S1 Table. Plasmids used in this study are summarized in S2 Table. Gene disruptions and tagging were performed by a standard PCR-based method ([49 (link)]. All chemical reagents were purchased from Fisher Scientific (NJ, USA), except for the following: Nitrogen bases were purchased from US Biological (Swampscott, MA); ProtoGel for Western blots from National Diagnostics (Atlanta, GA); Bacto peptone and Bacto agar from BD Difco (Franklin Lakes, NJ); salmon testes DNA, amino acids, 1-naphthyl phosphate, 2-nitrophenyl β-D-galactopyranoside and protease inhibitors from Sigma Aldrich (St. Louis, MO); glass beads from BioSpec Products (Bartlesville, OK); restriction enzymes and buffers from New England Biolabs (Ipswich, MA).
Antibodies used in this study included mouse monoclonal anti-GFP (Roche Diagnostics), rat anti-HA (Roche Diagnostics), mouse anti-HA (Covance), rabbit anti-G6PDH (Sigma), rabbit anti-Ape1 (a kind gift from Dr. Ohsumi), rabbit anti-Atg8 (a kind gift from Dr. Ohsumi), goat anti-rat HRP (Abcam), goat anti-rabbit HRP and goat anti-mouse HRP (GE Healthcare).

Lipatova Z., Gyurkovska V., Zhao S.F, & Segev N. (2020). Characterization of constitutive ER-phagy of excess membrane proteins. PLoS Genetics, 16(12), e1009255.

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Western Blot Analysis of C. elegans

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Cell lysate were prepared from synchronized population of L4 larvae and gravid adults. 50 µg lysate were loaded onto the precast polyacrylamide gel (Thermo Fisher Scientific), subjected to electrophoresis, and transferred onto the PVDF membrane (Bio-Rad) with Trans-Blot Turbo Transfer System (Bio-Rad). The primary antibodies used were anti-FLAG (M2, Sigma) and anti-alpha-Tubulin (Bio-Rad). The secondary antibodies were goat-anti-mouse HRP (Abcam) and goat-anti-rat HRP (Abcam) respectively.

Tang W., Seth M., Tu S., Shen E.Z., Li Q., Shirayama M., Weng Z, & Mello C.C. (2018). A sex chromosome piRNA promotes robust dosage compensation and sex determination in C. elegans. Developmental cell, 44(6), 762-770.e3.

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Yeast Strain and Antibody Protocol

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Yeast (Saccharomyces cerevisiae) strains used in this paper are summarized in Table S1. Plasmids used in this study are summarized in Table S2 Antibodies used in this study included mouse monoclonal anti-GFP (Roche Diagnostics), rat anti-HA (Roche Diagnostics), mouse anti-HA (Covance), rabbit anti-G6PDH
(Sigma), rabbit anti-Ape1 (a kind gift from Dr. Ohsumi), rabbit anti-Atg8 (a kind gift from Dr.
Ohsumi), goat anti-rat HRP (Abcam), goat anti-rabbit HRP and goat anti-mouse HRP (GE Healthcare).

Lipatova Z., Gyurkovska V., Zhao S.F., & Segev N. (2020). Characterization of Constitutive macro-ER-phagy.

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