Anti phosphorylated iκbα s32

Anti-Bcl2 and anti-phosphorylated mTOR (S2448) antibodies were obtained from Santa Cruz Biotechnology (Dallas, TX, USA). Anti-Caspase3, anti-Caspase7, anti-Caspase8, anti-β-actin, anti-phosphorylated PI3K (Y458/Y199), anti-phosphorylated Akt (S473), anti-p65, anti-PARP, anti-IκBα, anti-phosphorylated IκBα (S32), and anti-mTOR antibodies were purchased from Cell Signaling Technology (Danvers, MA, USA). MTT powder (1-(4,5-Dimethylthiazol-2-yl)-3,5-diphenylformazan) and METH powder were obtained from Sigma Chemical Co. (St. Louis, MO, USA). AnnexinV/PI apoptosis assay kit was purchased from BD Biosciences (San Diego, CA, USA). NE-PER Nuclear and Cytoplasmic Extraction Reagents Kit and ECL Western blotting detection reagents were obtained from Thermo Fisher Scientific (Waltham, MA, USA).

Anti-phosphorylated mTOR (S2448) antibodies and anti-Bcl2 were purchased from Santa Cruz Biotechnology (Dallas, TX, USA). Anti-p65, anti-PARP, anti-IκBα, anti-phosphorylated IκBα (S32), anti-β-actin, anti-phosphorylated ERK (T202/Y204), anti-ERK, anti-phosphorylated p38 (T180/Y182), anti-p38, anti-phosphorylated JNK (T183/Y185), anti-JNK, anti-phosphorylated c-Jun (S63), and anti-c-Jun were obtained from Cell Signaling Technology (Danvers, MA, USA). MTT (1-(4,5-Dimethylthiazol-2-yl)-3,5-diphenylformazan), phorbol 12-myristate 13-acetate (PMA), A23187, and dinitrochlorbenzene (DNCB) were purchased from Sigma Chemical Co. (St. Louis, MO, USA). Anti-human CD3 and CD28 antibodies were obtained from Bioxcell (West Lebanon, NH, USA). Human IL-2 ELISA kit was purchased from R&D systems (Minneapolis, MN, USA). AnnexinV staining dye and caspase3/7 staining dye for IncuCyte^® cell imaging system were obtained from Essen bio (Ann Arbor, MI, USA). Antibodies to CD69, CD25, and CD40L were purchased from ebiosciences (San Diego, CA, USA). NE-PER Nuclear and Cytoplasmic Extraction Reagents Kit was obtained from Thermo Fisher Scientific (Waltham, MA, USA). Mouse IgE ELISA kit was purchased from BD Biosciences (San Diego, CA, USA). House dust mite extract was obtained from Greer (Lenoir, NC, USA).

AnnexinV and caspase3/7 staining reagents for IncuCyte^® cell imaging system were obtained from Essen bio (Ann Arbor, MI, USA). AnnexinV/PI apoptosis assay kit was purchased from BD Biosciences (San Diego, CA, USA). Anti-CD3 and anti-CD28 for stimulation were obtained from Bioxcell (West Lebanon, NH, USA). Salinosporamide A, MTT (1-(4,5-Dimethylthiazol-2-yl)-3,5-diphenylformazan) powder, PMA (Phorbol 12-myristate 13-acetate), and A23187 was purchased from Sigma Chemical Co. (St. Louis, MO, USA). Carboxyfluorescein succinimidyl ester (CFSE) dye, ECL Western blotting detection reagents, and NE-PER Nuclear and Cytoplasmic Extraction Reagents Kit were obtained from Thermo Fisher Scientific (Waltham, MA, USA). PVDF membrane was obtained from Bio-Rad (Hercules, CA, USA). Anti-bcl-2, anti-β-actin, anti-cyclin A, anti-cyclin D1, and cyclin E antibodies were purchased from Santa Cruz Biotechnology (Dallas, TX, USA). Anti-caspase3, anti-caspase7, anti-p65, anti-PARP, anti-IκBα, anti-ERK, anti-p38, anti-JNK, anti-phosphorylated IκBα (S32), anti-phosphorylated ERK (T202/Y204), anti-phosphorylated p38 (T180/Y182), and anti-phosphorylated JNK (T183/Y185) antibodies were obtained from cell signaling technology (Danvers, MA, USA).