Uv 1800 double beam spectrophotometer

A definite weight of the nanoparticles (which theoretically contains the equivalent of 10 mg of drug) was dissolved in 100 mL solution of 0.1 N HCl. After continuous shaking for 10 minutes, the solution was filtered through a 0.2-µm membrane filter. The solution was appropriately diluted and the drug concentration was measured spectrophotometrically (Shimadzu UV-1800 double beam spectrophotometer, Kyoto, Japan) at a predetermined λ_max of 234 nm.45 (link)–47 The actual drug content was determined from which the drug encapsulation efficiency % (EE%) was calculated using the following equation:41 (link),44 (link),48 (link)
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$${\rm{EE \% = }}{{{\rm{Actual\,drug\,content}}} \over {{\rm{Theoretical\,drug\,content}}}}{\rm{ \times 100}}$$
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Absorption studies were performed on a Shimadzu UV 1800 double beam spectrophotometer, using 10 mm path length of quartz cuvettes. A Horiba Jobin Yvon FluoroMax - 4 spectrofluorometer was used to measure emission and excitation spectra with 5 nm in band width. The steady state fluorescence anisotropy (r_ss) is defined as [23] $r_{ss}=\frac{I_{VV}-G{I}_{VH}}{I_{VV}+2G{I}_{HH}}$ where I_vv and I_VH are the fluorescence intensities and the subscript indicates the vertical (V) and horizontal (H) orientation of the excitation and emission polarizer. G is the instrumental correction factor. $G=\frac{I_{HV}}{I_{HH}}$
The time-resolved fluorescence intensity decay profiles were collected using Horiba JobinYvon TCSPC Life Time System with FluoroHub single photon counting arrangement. Nano-LED of 280 nm (slit – 4) was used as excitation source. The pulse repetition rate was set at 1 MHz. The instrument response function was collected by using scattered medium, LUDOX AS40 colloidal silica. IBH software was used for the decay analysis. Decays were fitted to get a symmetrical distribution keeping χ² value at 0.99 ≤ χ² ≤ 1.2. The average lifetime was determined by using the following equation [23] , ${\langle \tau \rangle }_{avg}=\sum _{i=1}^n{\tau }_i{\alpha }_i$ where τ_i is the lifetime of a component having amplitude α_i.

UV–visible spectrophotometric experiments were recorded on a Shimadzu UV 1800 double beam spectrophotometer, using 10 mm quartz cuvettes. A Fluoromax-4 (Horiba Jobin Yvon, Germany) spectrofluorimeter was used to record the fluorescence spectra. Excitation and emission spectra were measured with 5 nm band width. Temperature control was achieved by circulating water through the jacketed cuvette holder from a refrigerated bath (Julabo, Germany). The steady state ${\mathit{r}}_{\mathrm{s}\mathrm{s}}$ is defined as [23] , ${\mathit{r}}_{\mathit{ss}}=\frac{{\mathit{I}}_{\mathit{VV}}-{\mathit{GI}}_{\mathit{VH}}}{{\mathit{I}}_{\mathit{VV}}+{2\mathit{GI}}_{\mathit{VH}}}$ where I_VV and I_VH are the fluorescence intensities and the subscript indicates the vertical (V) and horizontal (H) orientations of the excitation and emission polarizer. G is the instrumental correction factor, $\mathit{G}=\frac{{\mathit{I}}_{\mathit{HV}}}{{\mathit{I}}_{\mathit{HH}}}$