Trichostatin a tsa

Manufactured by Cell Signaling Technology

Sourced in United States

Trichostatin A (TSA) is a histone deacetylase (HDAC) inhibitor. It functions by inhibiting the activity of HDAC enzymes, which are responsible for the deacetylation of histones, leading to increased acetylation of histones and changes in gene expression.

Automatically generated - may contain errors

Lab products found in correlation

Lipofectamine 2000, by Thermo Fisher Scientific (3 mentions) Bortezomib, by Cell Signaling Technology (2 mentions) Nicotinamide, by Merck Group (2 mentions) Complete protease inhibitor cocktail, by Roche (2 mentions) 4 phenylbutyrate 4 pba, by Cell Signaling Technology (2 mentions) Aicar, by Cell Signaling Technology (2 mentions) Chloroquine cq, by Cell Signaling Technology (2 mentions) Mg132, by Cell Signaling Technology (2 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions) Lipofectamine 3000, by Thermo Fisher Scientific (1 mentions) Protein assay, by Bio-Rad (1 mentions) Protease inhibitor cocktail, by Cell Signaling Technology (1 mentions) Immobilon p, by Merck Group (1 mentions) Supersignal west pico, by Thermo Fisher Scientific (1 mentions) Femto chemiluminescence substrate, by Thermo Fisher Scientific (1 mentions) Nicotinamide nam, by Merck Group (1 mentions) Mg132, by Merck Group (1 mentions) Negative sirna, by Thermo Fisher Scientific (1 mentions) Sirna, by Cell Signaling Technology (1 mentions) Sirna, by Merck Group (1 mentions)

9 protocols using trichostatin a tsa

Cell Culture and Transfection Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

HEK293, A549, HCT116, and HT29 cells were maintained in Dulbecco’s modified Eagle’s medium with 10% fetal bovine serum and incubated at 37 °C in a humidified atmosphere of 5% CO₂. The cells were transfected using Lipofectamine 3000 (Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s instructions. Etoposide was purchased from Alexis (Lausen, Germany). Trichostatin A (TSA) was purchased from Cell Signaling Technology (Danvers, MA, USA). All other chemicals were purchased from Cayman Chemical (Ann Arbor, MI, USA) and Sigma Aldrich (St. Louis, MO, USA).

Lee H., Jung T.Y., Lim S.H., Choi E.J., Lee J, & Min D.S. (2021). Phospholipase D2 is a positive regulator of sirtuin 1 and modulates p53-mediated apoptosis via sirtuin 1. Experimental & Molecular Medicine, 53(9), 1287-1297.

+ Open protocol

+ Expand

Protein Extraction and Western Blotting

Check if the same lab product or an alternative is used in the 5 most similar protocols

Tissue extracts or cells were homogenized and lysed in NETN buffer (20 mM Tris-HCl, pH 8.0, 100 mM NaCl, 1 mM EDTA, 0.5% Nonidet P-40) supplemented with 50 mM β-glycerophosphate, 5 mM NaF, a protease inhibitor cocktail (Roche), 5 mM nicotinamide, and 5 μM trichostatin A (TSA, Cell Signaling). For PARylation analysis only, 100 μM of tannic acid was added to the lysis buffer. After 30 min of incubation at 4°C, the samples were centrifuged at 12,000 rpm for 10 min at 4°C. The protein concentration of the supernatant was determined by BioRad protein assay. The lysates were separated by SDS-PAGE, and electrophoretically transferred to PVDF membranes (Immobilon®-P; Millipore). Membranes were immunoblotted with the indicated antibodies as shown in key resources table. Enhanced chemiluminescence detection was performed using SuperSignal West Pico or Femto Chemiluminescence Substrate (Thermo Scientific). Films were scanned and densitometry was performed using ImageJ.

Tarragó M.G., Chini C.C., Kanamori K.S., Warner G.M., Caride A., de Oliveira G.C., Rud M., Samani A., Hein K.Z., Huang R., Jurk D., Cho D.S., Boslett J.J., Miller J.D., Zweier J.L., Passos J.F., Doles J.D., Becherer D.J, & Chini E.N. (2018). A potent and specific CD38 inhibitor ameliorates age-related metabolic dysfunction by reversing tissue NAD+ decline. Cell metabolism, 27(5), 1081-1095.e10.

+ Open protocol

+ Expand

Immunoprecipitation of Acetylation Complexes

Check if the same lab product or an alternative is used in the 5 most similar protocols

For cellular acetylation experiments, 293FT cells were co-transfected with combinations of pcDNA3.1-GFP, pcDNA3.1-HA-PGC-1α, pcDNA3.1-HDAC3-myc, pcDNA3.1-Flag-GCN5, and/or empty pcDNA3.1. 48 h after transfection, cells were harvested in ice-cold NP40 IP Lysis Buffer (20 mM Tris-HCl, 150 mM NaCl, 10% glycerol, 2 mM EDTA, 0.1% NP40, 10 mM NaF, with 1 mM DTT, 1 mM phenylmethylsulphonylflouride (PMSF), and supplemented with complete protease inhibitor cocktail (Roche), 4 μM Trichostatin A (TSA, Cell Signaling), 5 mM nicotinamide (Sigma), and 1 μM Bortezomib (Cell Signaling) on ice. Cell lysates were freeze/thawed in liquid nitrogen twice, and lysates cleared by centrifugation at 4°C for 10 minutes. 250 mg of protein was used for each immunoprecipitation and volume adjusted to 1ml with lysis buffer. Washed anti-HA Agarose beads (Pierce) were incubated with samples and rotation at 4°C for 4 h, followed by three brief washes with lysis buffer. Immunoprecipitated proteins were eluted in 2X loading buffer with boiling at 95°C for 5 minutes.

Emmett M.J., Lim H.W., Jager J., Richter H.J., Adlanmerini M., Peed L.C., Briggs E.R., Steger D.J., Ma T., Sims C.A., Baur J.A., Pei L., Won K.J., Seale P., Gerhart-Hines Z, & Lazar M.A. (2017). Histone Deacetylase 3 Prepares Brown Adipose Tissue For Acute Thermogenic Challenge. Nature, 546(7659), 544-548.

+ Open protocol

+ Expand

Transcriptional Regulation in Cell Lines

Check if the same lab product or an alternative is used in the 5 most similar protocols

INS-1 cells were cultured in RPMI 1640 medium with 11.1 mM glucose that contained 10% fetal bovine serum (FBS). HEK293T cells were cultured in Dulbecco’s modified Eagle’s medium with 10% FBS. Cells were treated with 200 nM trichostatin A (TSA; Cell Signaling Technology) for 20 h, 5 mM nicotinamide (NAM; Sigma) for 6 h, or 10 μM MG132 (Sigma) for 6 h in the presence of 5.6 mM glucose. Plasmid transfection was carried out by Lipofectamine 2000 (Invitrogen).

Zhang Y., Zhou F., Bai M., Liu Y., Zhang L., Zhu Q., Bi Y., Ning G., Zhou L, & Wang X. (2019). The pivotal role of protein acetylation in linking glucose and fatty acid metabolism to β-cell function. Cell Death & Disease, 10(2), 66.

+ Open protocol

+ Expand

Knockdown of HOPX in Neonatal Rat Ventricular Cardiomyocytes

Check if the same lab product or an alternative is used in the 5 most similar protocols

To knockdown HOPX expression, NRVCs were treated with siRNA (Rn01_00076610, Sigma-Aldrich, St. Louis, MO, USA) using Lipofectamine 2000 (Thermo Fisher Scientific) in optimum media (Thermo Fisher Scientific) for 3 h, and then cells were grown in 2% DMEM for 24 h. After 24 h of siRNA incubation, NRVCs were treated with ARVs or Trichostatin-A (TSA) (Cell signaling) for the next 24 h. Similarly, control cells were incubated with the negative siRNA (Thermo Fisher Scientific) and treated with ARVs and TSA.

Kashyap S., Rabbani M., de Lima I., Kondrachuk O., Patel R., Shafiei M.S., Mukker A., Rajakumar A, & Gupta M.K. (2021). HOPX Plays a Critical Role in Antiretroviral Drugs Induced Epigenetic Modification and Cardiac Hypertrophy. Cells, 10(12), 3458.

+ Open protocol

+ Expand

Immunoprecipitation of Acetylation Complexes

Check if the same lab product or an alternative is used in the 5 most similar protocols

+ Open protocol

+ Expand

NSCLC and SCLC Cell Lines Treatment

Check if the same lab product or an alternative is used in the 5 most similar protocols

Four NSCLC cell lines (H1650, PC9, H1299, and A549) and nine SCLC cell lines (DMS79, H2171, H1694, SHP77, H82, H446, H69, SW1271, and H841) were purchased from ATCC (Manassas, VA, USA) and cultured in DEME/F12 or RPMI‐1640 with 10% FBS and 5% GlutaMAX. Cells were revalidated by STR profiling if used for more than 3 years.
Trichostatin A (TSA) (Cell Signaling Technology, Danvers, MA, USA, #9950) was prepared in DMSO as a stock solution of 4 mm. Cell lines H1299 and SW1271 were seeded in 100 mm plates and treated by TSA at 400 nm and 2 mm, respectively. Cells were harvested after six hours of treatment and lysed for western blotting.

He T., Wildey G., McColl K., Savadelis A., Spainhower K., McColl C., Kresak A., Tan A.C., Yang M., Abbas A, & Dowlati A. (2020). Identification of RUNX1T1 as a potential epigenetic modifier in small‐cell lung cancer. Molecular Oncology, 15(1), 195-209.

+ Open protocol

+ Expand

Pharmacological Modulation of PTC Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

TPC-1 and K1 cells (human PTC cell lines) were obtained from the University of Colorado Cancer Center Cell Bank. All cells were cultured in RPMI 1640 medium supplemented with 10% FBS (Invitrogen, Carlsbad, CA, USA) at 37°C in a 5% CO₂ atmosphere. All experiments were performed with mycoplasma-free cells.
The ROS scavenger N-acetyl-L-cysteine (NAC), histone deacetylase (HDAC) inhibitor trichostatin A (TSA), AMPK pathway activator AICAR, autophagy inhibitor chloroquine (CQ), ER stress inhibitor 4-phenylbutyrate (4-PBA), and proteasome inhibitor MG-132 were all purchased from Cell Signaling Technology. The working concentration and time were confirmed by the manufacturer's instructions combined with the experimental requirements.

Yang Z., Huang R., Wei X., Yu W., Min Z, & Ye M. (2020). The SIRT6-Autophagy-Warburg Effect Axis in Papillary Thyroid Cancer. Frontiers in Oncology, 10, 1265.

+ Open protocol

+ Expand

Modulation of PTC cell lines

Check if the same lab product or an alternative is used in the 5 most similar protocols

Human PTC cell lines TPC-1 and K1 were purchased from the University of Colorado Cancer Center Cell Bank. All cells were cultured in RPMI 1640 medium supplemented with 10% FBS (Invitrogen, Carlsbad, CA, USA) at 37 °C in a 5% CO 2 atmosphere. ROS scavenger N-acetyl-L-cysteine (NAC), histone deacetylase HDAC inhibitor Trichostatin A (TSA), AMPK pathway activator AICAR, autophagy inhibitor chloroquine (CQ), ER-stress inhibitor 4-phenylbutyrate (4-PBA), proteasome inhibitor MG-132 were all purchased from Cell Signaling Technology. The work concentration and time were con rmed by instructions combined with experimental requirements.

Zhou Y., Huang R., Yu W., Min Z., & Ye M. (2020). SIRT6-Autophagy-Warburg effect axis in papillary thyroid cancer.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!