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Nsyb gal4

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NSyb-GAL4 is a genetic tool used in Drosophila research. It is a transgenic fly line that expresses the GAL4 transcriptional activator protein under the control of the Nsyb promoter, which drives expression in neurons.

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6 protocols using nsyb gal4

1

Drosophila Neuronal Cell Labeling and Imaging

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The following stocks were employed - y[1] w[*]; Mi{y[+mDint2]=MIC}Syt1[MI02197] (BDSC#35973) (Venken et al., 2011 (link)), y(1) w(*) Mi(y[+mDint2]=MIC) Sh(MI10885) (BDSC#56260), y(1) w(*);Mi(y[+mDint2]=MIC)Shal(MI10881) (BDSC#56089) (Venken et al., 2011 (link)), y(1) w(*); Mi(y[+mDint2]=MIC) Shab(MI00848) (BDSC#34115) (Venken et al., 2011 (link)), nSyb-GAL4(GMR57C10)(BDSC#39171) (Pfeiffer et al., 2008 (link)), repo-GAL4 (BDSC#7415) (Sepp et al., 2001 (link)), repo-QF2 (BDSC#66477) (Lin and Potter, 2016 (link)), Shaw-GAL4 (BDSC#60325) (Venken et al., 2011 (link); Li-Kroeger et al., 2018 (link)), Ddc-GAL4(BDSC#7009) (Feany and Bender, 2000 (link)), TH-GAL4(BDSC#8848) (Friggi-Grelin et al., 2003b (link)), Trh-GAL4(BDSC#38389) (Alekseyenko et al., 2010 (link)), UAS_H2A-GFP (Sherer et al., 2020 (link)), QUAS_H2B-mCherry (Sherer et al., 2020 (link)), brp-GAL4 (this manuscript), UAS_H2A::GFP-T2A-mKok::Caax (this manuscript). All lines were raised on standard media at 25°C, 50% RH.
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2

Genetic Manipulation of Drosophila Metabolism

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yw; dOgdh-T2A-Gal4, UAS-dOgdhWT-Flag (II), and UAS-OGDHWT-Flag (II) were generated as previously described.26 (link) The following stocks were obtained from the Bloomington Stock Center at Indiana University (BDSC) and VDRC: yw; n-syb-Gal4 (BDSC 51635), w; 20xUAS-IVS-mCD8:GFP (BDSC 32194), dOgdh RNAi (v12778). All flies were maintained at room temperature (21°C). All crosses were kept at 25°C.
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3

Transgenic Fly Strain Protocols

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Injections of transgenes for fly strains reported in this study were performed by Bestgene, Inc. Other fly stocks: repo-GAL4 (Sepp et al., 2001 (link)) (BDSC #7415), n-syb-GAL4 (Bushey et al., 2009 (link)), n-syb-QF2 (Riabinina et al., 2015 (link)), n-syb-LexA (Shearin et al., 2013 (link)), TrpA1-QF, and nompC-QF (Petersen and Stowers, 2011 (link)), QUAS-FLP (Potter et al., 2010 (link)), Brp-FRT-STOP-FRT-V5 (Chen et al., 2014 (link)), LexAop-GFP11 and UAS-CD4-GFP1-10 (Gordon and Scott, 2009 (link)), UAS-spGFP1-10::Nrx (Fan et al., 2013 (link)), UAS-syb::spGFP1-10 (Macpherson et al., 2015 (link)), vGlut-LexA (Baek et al., 2013 (link)), 24B-GAL4 (Brand and Perrimon, 1993 (link)).
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4

Drosophila Genetics Stock Inventory

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The following stocks were obtained from Bloomington Stock Center bloominton drosophila stock center (BDSC): Canton-S, w1118, Sirtuin12A-7-11 (BDSC, #8838), Sirtuin25B-2-35 (BDSC, #8839), Sirtuin4 (BDSC, #8840), DopEcR (BDSC, #10847), and Foxo_RNAi (BDSC, #32427). Dop1R1 (BDSC, #43667), UAS-Kir2.1 (BDSC, #6596), UAS-reaper (BDSC, #5823), nSyb-GAL4 (BDSC, #51635), Ppl-GAL4 (BDSC, #58768), Esg-GAL4 (BDSC, #26816), Adh (BDSC, #3976), Trhc01440 (BDSC, #10531), Tdc2-GAL4 (BDSC, #9313), GAL4 covering a broader subset of ring neurons (BDSC, #39805), R2,3d,4 m-GAL4 (BDSC, #SS02709), R1-GAL4 (BDSC, #49661), R2-GAL4 (BDSC, #48343), R3d-GAL4 (BDSC, #40074), R4d-GAL4 (BDSC, #48487), R4m-GAL4 (BDSC, #39209), R5-GAL4 (BDSC, #39198), RelE38, and Dif mutants were gifts from M. Hedengren. NPFsk2 was provided by S. Kondo, and ppk28 was a gift from K. Scott. E. Johnson provided the Crz-GAL4 flies. DIlp2,3,5 and foxo94 were a gift from L. Partridge, and UAS-Tsc1-Tsc2 were from J. H. Lee. Akh, AkhR, and bmm mutant flies were a gift from M. Dus. Aldh∆24 and CrzR01 mutant flies were provided by F. James and J. Park, respectively. TbhnM18 flies were provided by K. Iliadi, and PoxnM22−B5XB mutants were provided by J. Alcedo. Gr63a mutant flies were a gift from A. Ray. Serotonin receptor mutant flies were a gift from Y. Rao. Gr63a/orco/Ir8a/Ir25a mutant flies were provided by R. Bento.
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5

Drosophila Genetic Reference Panel

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The Drosophila melanogaster Genetic Reference Panel (DGRP) strains were from the Bloomington Drosophila Stock Center (BDSC). The UAS-Syp RNAi lines (#33011, #33012) and the genetic control line were from the Vienna Drosophila Resource Center (VDRC). The actin-Gal4 (#4414) and the pan-neuronal driver nSyb-Gal4 (#51635) were from the BDSC. Unless stated otherwise, all flies were reared on the standard cornmeal medium from FlyKitchen at the University of Chicago at 25 °C under a 12-hr:12-hr light: dark cycle with the light on at 06:00 and off at 18:00.
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6

Comprehensive Fly Line Database for Neuroscience

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The fly lines used in this study are listed below, and unless indicated otherwise, each line was made in-house, with details provided in the section below: UAS-Cac-kisser-pHerry; UAS-Cac-kisser-FAP; UAS-Sh-kisser-FAP; UAS-PMCA-kisser-pHerry; UAS-PMCA-kisser-FAP; UAS-TagBFP (BDSC); CacsfGFP-N [developed by Gratz et al. (2018) (link)]; UAS-mTFP1-CEPIA3; UAS-iRFP670-RCaMP1a; 24B-Gal4 (BDSC); nSyb-Gal4 (BDSC).
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