HeLa cells grown on coverslips with or without chlamydial infection were fixed and permeabilized for immunostaining as described previously [41 (link)–43 (link)]. Hoechst (blue, Sigma) was used to visualize nuclear DNA. For titrating IFUs from mouse vaginal swab and oviduct tissue homogenate samples, a mouse anti-chlamydial LPS antibody (clone# MB5H9, unpublished observation) plus a goat anti-mouse IgG conjugated with Cy3 (red; Jackson ImmunoResearch Laboratories, Inc., West Grove, PA) were used to visualize chlamydial inclusions. All immunofluorescence-labeled samples were observed under an Olympus AX-70 fluorescence microscope equipped with multiple filter sets (Olympus, Melville, NY).
Goat anti mouse igg conjugated with cy3 red
Manufactured by Jackson ImmunoResearch
Goat anti-mouse IgG conjugated with Cy3 (red) is a secondary antibody used to detect the presence of mouse immunoglobulin G (IgG) in various immunoassays and research applications. The antibody is labeled with the Cy3 fluorescent dye, which emits a red signal upon excitation.
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2 protocols using goat anti mouse igg conjugated with cy3 red
1
Chlamydia Inclusion Immunostaining
2
Chlamydia Immunostaining and Quantification
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HeLa cells grown on coverslips with chlamydial infection were fixed and permeabilized for immunostaining as described previously [16] (link), [17] (link). Hoechst dye (blue, Sigma) was used to visualize nuclear DNA. For titrating IFUs from swab and tissue homogenate samples, a mouse anti-chlamydial LPS antibody (clone# MB5H9, unpublished observation) plus a goat anti-mouse IgG conjugated with Cy3 (red; Jackson ImmunoResearch Laboratories, Inc., West Grove, PA) were used to visualize chlamydial inclusions. All immunofluorescence-labeled samples were observed under an Olympus AX-70 fluorescence microscope equipped with multiple filter sets (Olympus, Melville, NY).
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