Anti erp72

Manufactured by Cell Signaling Technology

Sourced in United States

Anti-ERp72 is an antibody that recognizes the ERp72 protein, which is a member of the protein disulfide isomerase (PDI) family and functions as a molecular chaperone in the endoplasmic reticulum (ER).

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Close spelling variants of this product

ERp72 (5 citations) Rabbit anti-ERp72 (2 citations)

4 protocols using anti erp72

Investigating Calcium-Mediated ER Stress Responses

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Reagents used were AZC (TCI Europe, Zwijndrecht, Belgium; # A1043 or Acros Organics, Geel, Belgium; # 105142500), bafilomycin A1 (Sanbio, Uden, The Netherlands; # 11038-500), ethylene glycol tetraacetic acid (Acros Organics; # 409910250), BAPTA-AM (Thermo Fisher Scientific, Waltham, MA, USA; # B6769), TG (Alomone labs, Jerusalem, Israel; # T-650), Fura-2 AM (Life Technologies, Carlsbad, CA, USA; # F1221), staurosporine (LC Labs, Woburn, MA, USA; # S9300), and AMG PERK 44 (Tocris, Abingdon, U.K.; # 5517).
Primary antibodies used were anti-ATF6 (Cell Signaling Technology, Leiden, The Netherlands; # 65880), anti-BiP (Cell Signaling Technologies; # 3183), anti-eIF2α (Cell Signaling Technology; # 9722), anti-phospho-eIF2α (Cell Signaling Technology; # 3398), anti-ERp57 (Cell Signaling Technology; # 2881), anti-ERp72 (Cell Signaling Technology; # 2798), anti-GAPDH (Sigma-Aldrich, St. Louis, MO, USA; # G8795), anti-IP₃R (Rbt475 [42 (link)] recognizing all IP₃R isoforms), anti-LC3 (Cell Signaling Technology; # 2775), anti-MCU (Sigma-Aldrich; # HPA016480), anti-PARP (Cell Signaling Technology; # 9532), anti-PMCA (Thermo Fisher Scientific; # MA3-914) recognizing all PMCA isoforms, anti-SERCA2B (Cell Signaling Technology; # 4435), and anti-vinculin (Sigma-Aldrich; # V-9131).

Roest G., Hesemans E., Welkenhuyzen K., Luyten T., Engedal N., Bultynck G, & Parys J.B. (2018). The ER Stress Inducer l-Azetidine-2-Carboxylic Acid Elevates the Levels of Phospho-eIF2α and of LC3-II in a Ca2+-Dependent Manner. Cells, 7(12), 239.

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Fluorescence Immunocytochemistry for Cell Analysis

Cited 1 time

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Fluorescent immunocytochemistry was performed as previously described (Daniels et al., 2017 (link)). Cells were fixed for 15 min with 4% paraformaldehyde (Fisher), permeabilized with 0.1% Triton-X, and blocked with 10% goat serum. Cells were stained using primary anti-puromycin (Clone 12D10, EMD Millipore) or anti-ERp72 (Cell Signaling Technologies) for 1 h at room temperature, followed by secondary goat anti-mouse AlexaFluor594 (Abcam) or goat anti-rabbit AlexaFluor405 (Abcam). Dead cells were visualized via staining with the fixable viability dye Zombie Green (BioLegend).

Orozco S.L., Daniels B.P., Yatim N., Messmer M.N., Quarato G., Chen-Harris H., Cullen S.P., Snyder A.G., Ralli-Jain P., Frase S., Tait S.W., Green D.R., Albert M.L, & Oberst A. (2019). RIPK3 Activation Leads to Cytokine Synthesis that Continues after Loss of Cell Membrane Integrity. Cell reports, 28(9), 2275-2287.e5.

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Immunoblot Antibodies for Inflammasome Research

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For Immunoblot: Cell Signaling Technology: anti-β-Actin (3700), anti-ERP72 (5033), anti-S6K (2708), anti-α-Tubulin (3873), anti-FLAG (14793). eBiosciences: anti-Caspase-1 p20 (14-9832-82), anti-AIM2 (14-6008-93). Santa Cruz Technologies: anti-ASC (sc-22514-R), anti-TGN38 (sc-166594), anti-HistoneH1 (sc393358). Sigma: anti-Caspase-11 (C1354), anti-GasderminD (G7422), anti-FLAGM2 (F3165). Adipogen: anti-NLRP3 (AG-20B-0014-C100), anti-ASC (AG-25B-0006-C100). Millipore: anti-Phospho-p62 S403 (MABC186). Research & Development: anti-IL-1β (AF-401-NA). Roche: anti-HA (11815016001). Abcam: anti-GasderminD (ab219800), anti-NEK7 (ab133514), anti-Calreticulin (ab92516), anti-GM130 (ab52649), anti-CathepsinS (ab232740), anti-LPS (ab35654). Biolegend: anti-LAMP1 (121602). For immunoprecipitation of HA-NLRP3: Cell Signaling: anti-HA (3724).

Moretti J., Jia B., Hutchins Z., Roy S., Yip H., Wu J., Shan M., Jaffrey S.R., Coers J, & Blander J.M. (2022). Caspase-11 Interaction with NLRP3 Potentiates the Noncanonical Activation of the NLRP3 Inflammasome. Nature immunology, 23(5), 705-717.

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Immunoblot Antibodies for Inflammasome Research

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