2550 spectrophotometer

The particle sizes of the prepared nanoparticles were calculated from the X-ray diffraction data (Philips PW1710 diffractometer, CuK_α radiation, Holland) using the Scherrer equation. The surface morphologies, particle sizes, and various contours of the nanocatalyst powders were analyzed by transmission electron microscopy (FEI TITAN G² 80-300) operated at 300 keV. Diffuse reflectance UV-Vis spectra of the nanocatalysts were recorded using a Shimadzu 2550 spectrophotometer equipped with an integrating sphere accessory using BaSO₄ as the reference material. Raman spectra were recorded using an inVia Raman spectrometer (Renishaw, UK) operating at a wavelength at 785 nm with a resolution of 1 cm⁻¹. The magnetization measurements were performed with a physical property measurement system (Quantum Design PPMS-II) under ambient conditions. The total organic carbon (TOC) for all the samples was analyzed by direct injection of the filtered sample solution into a TOC analyzer (Shimadzu TOC-VCPH model). Prior to the TOC analysis, the instrument was calibrated with potassium hydrogen phthalate. TOC₀ is the TOC measured after the equilibrium adsorption of the dye on the nanocatalyst surface, and the TOCs obtained at various irradiation times are denoted as TOC_t.

The ¹H NMR spectra of the microgel was studied using a VARIAN 400 spectrometer (400 MHz). Transmission electron microscopy (TEM) images were obtained using a JEOL (Tokyo, Japan) 2100 microscope operated at 200 kV. Field emission scanning electron microscopy (FESEM) studies were performed on a JSM 7600 F microscope (JEOL, Tokyo, Japan) at 5 kV. UV-Vis spectra of the samples were recorded using a Shimadzu (Tokyo, Japan) 2550 spectrophotometer. Finally, the size of the composite particles dispersed in water (20 mg/mL) was determined by Malvern Nano ZS dynamic light scattering (DLS) varying from 20 to 50 °C.

The sample diluents for P-selectin UV spectrum were from rat ELISA kit (Rat P-selectin ELISA Kit; Wuhan Huamei Biotech Co., Ltd., Wuhan, Hubei Province, P. R. China). A solution of P-selectin in sample diluents was prepared (300 ng/mL), from which 300 μL was added into six eppendorf tubes. The control tube only contains 300 μL of sample diluents. The sample tube contains 300 μL solution of P-selectin in sample diluents (300 ng/mL) and 10 μL solution of THPDTPI in sample diluents (final concentration: 0.5, 4.8, 5.0 and 5.4 μM). All tubes were incubated at room temperature for 12 h then received UV test on a Shimadzu 2550 spectrophotometer.