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Horseradish peroxidase conjugated goat anti mouse immunoglobulin ig g

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Horseradish peroxidase-conjugated goat anti-mouse immunoglobulin (Ig) G is a conjugate used in immunoassays and other immunochemical techniques. It consists of goat-derived antibodies specific to mouse IgG, which are coupled to the enzyme horseradish peroxidase.

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Lab products found in correlation

Horseradish peroxidase conjugated goat anti rabbit igg, by ICN Biomedicals (2 mentions) Phospho ros1, by Cell Signaling Technology (1 mentions) P akt1 2 3 antibody ser 473 r sc 7985 r, by Santa Cruz Biotechnology (1 mentions) C myc antibody 9e10, by Santa Cruz Biotechnology (1 mentions) Mcl 1 antibody s 19, by Santa Cruz Biotechnology (1 mentions) Gapdh antibody g 9, by Santa Cruz Biotechnology (1 mentions)

2 protocols using horseradish peroxidase conjugated goat anti mouse immunoglobulin ig g

1

Western Blotting Methodology in ROS1 Signaling

Cited 2 times
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Western blotting was performed as described previously.24 (link)–26 (link) Monoclonal antibody (mAb) against glyceraldehyde-3-phosphate dehydrogenase (GAPDH) antibody (G-9) (sc-365062) was used to standardize cytosolic protein loading on the sodium dodecyl sulfate-polyacrylamide gel electrophoresis. ROS1 (D4D6) rabbit mAbs from Cell Signaling Technology (catalog no: 3287) were used to recog-nize FIG-ROS1 and Phospho-ROS1 activity (Tyr2274) (catalog no: 3078). Anti-GOPC/FIG antibody (MyBioSource: MBS421395) was used to detect FIG expression. p-Met antibody (Tyr 1365) (sc-3408), p-Met (F-5) (sc-377548), p-ALK antibody (Tyr 1586) (sc-109905), Bim (H-191) (sc-11425), survivin (D-8) (sc-17779), Mcl-1 antibody (S-19) (sc-819), and p-Akt1/2/3 antibody (Ser 473)-R (sc-7985-R) were purchased from Santa Cruz Biotechnology, Inc. The secondary antibodies were horseradish peroxidase-conjugated goat anti-mouse immunoglobulin (Ig) G (ICN Biomedicals) and horseradish peroxidase-conjugated goat anti-rabbit IgG (ICN Biomedicals).
Das A., Cheng R.R., Hilbert M.L., Dixon-Moh Y.N., Decandio M., Vandergrift WA I.I.I., Banik N.L., Lindhorst S.M., Cachia D., Varma A.K., Patel S.J, & Giglio P. (2015). Synergistic Effects of Crizotinib and Temozolomide in Experimental FIG-ROS1 Fusion-Positive Glioblastoma. Cancer Growth and Metastasis, 8, 51-60.
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2

Western Blotting for Protein Analysis

Cited 1 time
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Western blotting was performed as we described previously [26 (link)–29 (link)]. GAPDH was used to standardize cytosolic protein loading on the sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Mcl-1 antibody (S-19) (sc-819), c-Myc antibody (9E10) (sc-40), cyclin D1 antibody (H-295) (sc-753), VEGF antibody (147) (sc-507), LRP5 antibody (B-9) (sc-390267), frizzled-4 antibody (H-120) (sc-135108), p-GSK-3α/β antibody (6D3) (sc-81496), Wnt-5α/β antibody ( sc-376249), Bcl-xL antibody (H-5) ( sc-8392), TSPAN12 (sc-133810), β-catenin antibody (H-102) (sc-7199), Bax antibody (N-20): sc-493, p-Akt1/2/3 antibody (Ser 473)-R (sc-7985-R), GAPDH antibody (G-9) (sc-365062), and caspase-3 p11 antibody (C-6) (sc-271759) were purchased from Santa Cruz Biotech (Santa Cruz, Calif). The secondary antibodies were horseradish peroxidase-conjugated goat antimouse immunoglobulin (Ig) G (ICN Biomedicals, Aurora, Ohio) and horseradish peroxidase-conjugated goat antirabbit IgG (ICN Biomedicals).
Das A., Miller R., Lee P., Holden C.A., Lindhorst S.M., Jaboin J., Vandergrift WA I.I.I., Banik N.L., Giglio P., Varma A.K., Raizer J.J, & Patel S.J. (2015). A novel component from citrus, ginger, and mushroom family exhibits antitumor activity on human meningioma cells through suppressing the Wnt/β-catenin signaling pathway. Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine, 36(9), 7027-7034.
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