A Trace Ultra 1300 gas chromatograph (Thermos Scientific, Waltham, MA, USA) fitted with a TG-5 ms column (30 m × 0.25 mm i.d., film thickness, 0.25 µm, Thermo Scientific, Milan Italy) and an ODP 3 olfactory Detector Port (Gerstel, Mulheim, Germany), with additional supply of humidified purge air, was operated as earlier reported by Lasekan [35 (link)]. The split ratio between the sniffing port and the FID detector was 1:1. Two replicate samples were sniffed by three trained panellists who presented normalized responses, with strong agreement with one another.
Trace ultra 1300
Manufactured by Thermo Fisher Scientific
Sourced in United States, Germany
The Trace Ultra 1300 is a gas chromatograph (GC) instrument designed for analytical laboratory applications. It provides high-performance separation and detection of a wide range of organic compounds. The Trace Ultra 1300 features advanced technology and design to ensure reliable and accurate results.
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5 protocols using trace ultra 1300
1
Gas Chromatography-Olfactory Analysis of Volatile Compounds
2
Aroma-active Compound Identification in Fry Bread
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In order to identify the aroma-active constituents in the fry bread extracts, an olfactory detection port ODP-3 (Gestalt, Mulheim, Germany) which was connected to a Trace Ultra 1300 gas chromatograph (Thermos Scientific, Waltham, MA, USA) was used to conduct the sniffing test. The GC-O system was fitted with a DB-Wax column (30 m × 0.32 mm and 0.5 μm film thickness, J & W Scientific, Folsom, CA, USA). Sniffing was conducted as described previously [30 (link)]. Three experienced panelists (two females and a male) with strong gustative and olfactory responses in earlier sessions were used for the sniffing test. The sniffing analysis was divided into three sessions of 20 min, and each assessor participated in the exercise. All analyses were repeated in triplicate by each assessor.
3
Gas Chromatography-Olfactory Analysis of Aroma Compounds
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A Trace Ultra 1300 gas chromatograph (Thermo Scientific, Waltham, MA, USA) fitted with a DB-FFAP column (30 m × 0.32 mm i.d., film thickness, 0.25 µm, Scientific Instrument Services, Inc., Ringoes, NJ) and an ODP 3 olfactory Detector Port (Gerstel, Mulheim, Germany), with additional supply of humidified purge air, was operated as earlier reported by Lasekan et al. [25 (link)]. The split ratio between the sniffing port and the FID detector was 1:1. Two replicate samples were sniffed by three trained panellists who presented normalised responses, reproducibility and agreement with one another. The GC–O analysis was divided into three parts of 20 min and each panellist participated in the sniffing. An aroma note is valid only when the three panellists were able to detect the odour note.
4
Aroma Profiling of Complex Samples
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A Trace Ultra 1300 gas chromatograph (Thermo Scientific, Waltham, MA, USA) fitted with either a DB-FFAP or DB-5 column 1:(30 m × 0.32 mm i.d., film thickness, 0.25 µm, Scientific Instrument Services, Inc., Ringoes, NJ) and an ODP 3 olfactory Detector Port (Gerstel, Mulheim, Germany), with additional supply of humidified purge air, was operated as earlier reported by Lasekan et al. [21 (link)]. The split ratio between the sniffing port and the FID detector was 1:1. Two replicate samples were sniffed by three trained panelists who presented normalized responses, with strong agreement with one another.
5
Olfactory Analysis of Bagel Aroma Compounds
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To further identify the aroma constituents in the bagel extracts, an olfactory detection port ODP-3 (Gerstel, Mulheim, Germany) connected to a Trace Ultra 1300 gas chromatograph (Thermos Scientific, Waltham, MA, USA) was used. The GC–O system was fitted with a DB-FFAP column (30 m × 0.32 mm i.d; film thickness, 0.25 µm, Scientific Instrument Services, Inc., Ringoes, NJ). The GC–O conditions are the same as reported in “Analysis of volatile constituents ” section. The effluent was split 1:1. Sniffing was conducted as described previously [39 (link)]. Three experienced panelists (two females and a male) with strong gustative and olfactory responses in earlier sessions were used for the sniffing test. The sniffing analysis was divided into three sessions of 20 min and each assessor participated in the exercise. All analyses were repeated in triplicate by each assessor.
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