Anti-ZIKV envelope (catalog number GTX133314) and anti-ZIKV NS5 (catalog number GTX133312) were obtained from GeneTex; anti-ERK1/2 (catalog number 9102S), anti-phospho-ERK1/2 (P-ERK1/2) (catalog number 4370S), anti-AKT (catalog number 9272), and anti-phospho-AKT (catalog number 9271S) were obtained from Cell Signaling; anti-CCR3 (catalog number PA5-19859) and anti-CCR5 (catalog number PA5-78949) were obtained from Invitrogen; anti-CCL5 (catalog number K1014) and the IgG isotype control (VP16, catalog number F249) were obtained from Santa Cruz; and anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (catalog number G9545) was obtained from Sigma-Aldrich. Neutralizing antibodies to CCL5 (catalog number AF-278-NA), CCR3 (catalog number MAB155), CCR5 (catalog number MAB182), and CCL5/RANTES DuoSet ELISA kits were obtained from R&D Systems (12 (link)).
Anti erk1 2
Manufactured by GeneTex
Anti-ERK1/2 is a primary antibody product that detects extracellular signal-regulated kinases 1 and 2 (ERK1/2). ERK1/2 are serine/threonine-protein kinases that play a crucial role in the regulation of various cellular processes.
Automatically generated - may contain errors
Lab products found in correlation
Anti phospho akt, by Cell Signaling Technology (1 mentions)
Anti glyceraldehyde 3 phosphate dehydrogenase gapdh, by Merck Group (1 mentions)
Anti akt, by Cell Signaling Technology (1 mentions)
Anti phospho erk1 2 p erk1 2, by Cell Signaling Technology (1 mentions)
Anti β actin, by Merck Group (1 mentions)
Ecl western blotting detection reagent, by GE Healthcare (1 mentions)
Mouse monoclonal anti vegfr 1 clone d2, by Santa Cruz Biotechnology (1 mentions)
Antimouse or anti rabbit ig horseradish peroxidase secondary antibodies, by GE Healthcare (1 mentions)
2 protocols using anti erk1 2
1
ZIKV Envelope and NS5 Antibody Detection
2
Western Blot Analysis of VEGFR-1 and EGFR
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Proteins were run in 10% SDS-polyacrylamide gels and transferred to supported nitrocellulose membranes by standard techniques. Immunodetection was performed using the following antibodies: mouse monoclonal anti-VEGFR-1 (clone D2, 1:500; Santa Cruz Biotechnology, Santa Cruz, CA); mouse monoclonal anti-EGF receptor (EGFR) (528, 1:1000; Santa Cruz Biotechnology); mouse monoclonal antibody anti-EGFRvIII (L8A4; 1:1000; Absolute Antibody, Oxford, UK); rabbit polyclonal anti-phosphorylated VEGFR-1 at tyrosine 1213 (1:500; R&D Systems); rabbit polyclonal anti-Erk1&2 (1:1000; Genetex, Irvine, CA); rabbit polyclonal anti-phospho-Erk1&2 (Thr/Tyr185/187, 1:1000; Invitrogen); or rabbit polyclonal anti-β-actin (1:10,000; Sigma Aldrich) primary antibodies. Anti-mouse or anti-rabbit Ig/Horseradish peroxidase secondary antibodies and ECL Western blotting detection reagents from GE Healthcare (Milan, Italy) were used to identify the proteins of interest.
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