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Mouse il 3

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Mouse IL-3 is a laboratory reagent used in research. It is a cytokine that plays a role in the regulation of hematopoiesis. The specific functions and applications of Mouse IL-3 should be further investigated by the researcher.

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Lab products found in correlation

Streptomycin, by Roche (4 mentions) Penicillin, by Roche (4 mentions) Penicillin streptomycin, by Roche (2 mentions) Penicillin streptomycin, by Immunotools (2 mentions) Iscove modified dulbecco media (imdm), by Thermo Fisher Scientific (2 mentions) Fetal calf serum (fcs), by Merck Group (2 mentions) Skbr3, by American Type Culture Collection (2 mentions) Dld 1, by American Type Culture Collection (2 mentions) Penicillin, by Immunotools (2 mentions) Streptomycin, by Immunotools (2 mentions) Penicillin, by Thermo Fisher Scientific (2 mentions) Streptomycin, by Thermo Fisher Scientific (2 mentions) Imdm medium, by Thermo Fisher Scientific (2 mentions) Roswell park memorial institute (rpmi), by Immunotools (2 mentions) Cd8 t cell isolation kit, by Miltenyi Biotec (2 mentions) Glutamax, by Merck Group (2 mentions) Recombinant hil 2, by Novartis (2 mentions) Mem non essential amino acid, by Merck Group (2 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (2 mentions) Roswell park memorial institute (rpmi), by Thermo Fisher Scientific (2 mentions)

5 protocols using mouse il 3

1

Culturing Various Cell Lines

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HAP1 cells have been described previously43 (link). A375, A431, A549, DLD1, JY, Raji, RKO and SKBR3 cells were purchased from American Type Culture Collection (ATCC). B16F10 cells were kindly provided by D. Peeper. Ba/F3 cells are bone-marrow-derived, immortalized cells which are IL-3-dependent, as described in44 (link). NKIRTIL006 cell line was generated from a patient treated at the Netherlands Cancer Institute.
HAP1 cells were cultured in IMDM (ThermoFisher Scientific) supplemented with 10% Fetal Calf Serum (FCS, Sigma), 100 U/mL penicillin (Roche) and 100 μg/mL streptomycin (Roche) (penicillin/streptomycin) and L-glutamine. NKIRTIL006 cells were cultured in IMDM supplemented with 10% FCS and penicillin/streptomycin. A375, A549 and B16F10 were cultured in DMEM supplemented with 10% FCS and penicillin/streptomycin. A431, DLD1, Raji and JY cells were cultured in RPMI supplemented with 10% FCS and penicillin/streptomycin. Ba/F3-Her2 cells were cultured in RPMI supplemented with 10% FCS, penicillin/streptomycin, 0.2 ng/mL mouse IL-3 (Immunotools) and 5.0 μg/mL puromycin. SKBR3 cells were cultured in IMDM supplemented with 20% FCS and 100 U/mL penicillin/streptomycin. Cells were cultured at 37°C and 5% CO2.
Logtenberg M.E., Jansen J.H., Raaben M., Toebes M., Franke K., Brandsma A.M., Matlung H.L., Fauster A., Gomez-Eerland R., Bakker N.A., van der Schot S., Marijt K.A., Verdoes M., Haanen J.B., van den Berg J.H., Neefjes J., van den Berg T.K., Brummelkamp T.R., Leusen J.H., Scheeren F.A, & Schumacher T.N. (2019). Glutaminyl cyclase is an enzymatic modifier of the CD47- SIRPα axis and target for cancer immunotherapy. Nature medicine, 25(4), 612-619.
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2

Culturing Various Cell Lines

Cited 1 time
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HAP1 cells have been described previously43 (link). A375, A431, A549, DLD1, JY, Raji, RKO and SKBR3 cells were purchased from American Type Culture Collection (ATCC). B16F10 cells were kindly provided by D. Peeper. Ba/F3 cells are bone-marrow-derived, immortalized cells which are IL-3-dependent, as described in44 (link). NKIRTIL006 cell line was generated from a patient treated at the Netherlands Cancer Institute.
HAP1 cells were cultured in IMDM (ThermoFisher Scientific) supplemented with 10% Fetal Calf Serum (FCS, Sigma), 100 U/mL penicillin (Roche) and 100 μg/mL streptomycin (Roche) (penicillin/streptomycin) and L-glutamine. NKIRTIL006 cells were cultured in IMDM supplemented with 10% FCS and penicillin/streptomycin. A375, A549 and B16F10 were cultured in DMEM supplemented with 10% FCS and penicillin/streptomycin. A431, DLD1, Raji and JY cells were cultured in RPMI supplemented with 10% FCS and penicillin/streptomycin. Ba/F3-Her2 cells were cultured in RPMI supplemented with 10% FCS, penicillin/streptomycin, 0.2 ng/mL mouse IL-3 (Immunotools) and 5.0 μg/mL puromycin. SKBR3 cells were cultured in IMDM supplemented with 20% FCS and 100 U/mL penicillin/streptomycin. Cells were cultured at 37°C and 5% CO2.
Logtenberg M.E., Jansen J.H., Raaben M., Toebes M., Franke K., Brandsma A.M., Matlung H.L., Fauster A., Gomez-Eerland R., Bakker N.A., van der Schot S., Marijt K.A., Verdoes M., Haanen J.B., van den Berg J.H., Neefjes J., van den Berg T.K., Brummelkamp T.R., Leusen J.H., Scheeren F.A, & Schumacher T.N. (2019). Glutaminyl cyclase is an enzymatic modifier of the CD47- SIRPα axis and target for cancer immunotherapy. Nature medicine, 25(4), 612-619.
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3

Isolation and Culture of CD8+ T Cells

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CD8+ T cells were isolated from PBMCs using the CD8+ T cell Isolation Kit (Miltenyi Biotec). PBMCs and CD8+ T cells were cultured in RPMI (Gibco) supplemented with 10% human serum (HS, Sigma), penicillin (100 U/ml, Roche), streptomycin (100 μg/ml, Roche) and recombinant hIL-2 (60 IU/ml, Novartis). BA/F3 cells (kindly provided by J. Leusen, UMC Utrecht, The Netherlands40) were cultured in RPMI supplemented with 10% FCS, penicillin (100 U/ml), streptomycin (100 μg/ml) and 0.2 ng/mL mouse IL-3 (Immunotools). OVCAR5 cells (kindly provided by F. Scheeren, The Netherlands Cancer Institute, The Netherlands) were cultured in IMDM medium (Gibco) supplemented with 10% FCS, penicillin (100 μg/ml), streptomycin (100 μg/ml) and GlutaMax (1×, Gibco). Viable human tumor tissue pieces of ~1-2mm3 were thawed in prewarmed DMEM (Gibco) supplemented with 10% FCS, penicillin (100 U/ml), streptomycin (100 μg/ml), sodium pyruvate (1 mM, Sigma), MEM non-essential amino acids (1x, Sigma) and GlutaMax (1x). Tumor tissue was subsequently washed three times by thoroughly submerging and shaking the tissue pieces in fresh prewarmed medium.
van der Leun A.M., Hoekstra M.E., Reinalda L., Scheele C.L., Toebes M., van de Graaff M.J., Chen L.Y., Li H., Bercovich A., Lubling Y., David E., Thommen D.S., Tanay A., van Rheenen J., Amit I., van Kasteren S.I, & Schumacher T.N. (2021). Single Cell Analysis of Regions of Interest (SCARI) using a photo-sensitive tag. Nature chemical biology, 17(11), 1139-1147.
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4

Isolation and Culture of CD8+ T Cells

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CD8+ T cells were isolated from PBMCs using the CD8+ T cell Isolation Kit (Miltenyi Biotec). PBMCs and CD8+ T cells were cultured in RPMI (Gibco) supplemented with 10% human serum (HS, Sigma), penicillin (100 U/ml, Roche), streptomycin (100 μg/ml, Roche) and recombinant hIL-2 (60 IU/ml, Novartis). BA/F3 cells (kindly provided by J. Leusen, UMC Utrecht, The Netherlands40) were cultured in RPMI supplemented with 10% FCS, penicillin (100 U/ml), streptomycin (100 μg/ml) and 0.2 ng/mL mouse IL-3 (Immunotools). OVCAR5 cells (kindly provided by F. Scheeren, The Netherlands Cancer Institute, The Netherlands) were cultured in IMDM medium (Gibco) supplemented with 10% FCS, penicillin (100 μg/ml), streptomycin (100 μg/ml) and GlutaMax (1×, Gibco). Viable human tumor tissue pieces of ~1-2mm3 were thawed in prewarmed DMEM (Gibco) supplemented with 10% FCS, penicillin (100 U/ml), streptomycin (100 μg/ml), sodium pyruvate (1 mM, Sigma), MEM non-essential amino acids (1x, Sigma) and GlutaMax (1x). Tumor tissue was subsequently washed three times by thoroughly submerging and shaking the tissue pieces in fresh prewarmed medium.
van der Leun A.M., Hoekstra M.E., Reinalda L., Scheele C.L., Toebes M., van de Graaff M.J., Chen L.Y., Li H., Bercovich A., Lubling Y., David E., Thommen D.S., Tanay A., van Rheenen J., Amit I., van Kasteren S.I, & Schumacher T.N. (2021). Single Cell Analysis of Regions of Interest (SCARI) using a photo-sensitive tag. Nature chemical biology, 17(11), 1139-1147.
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5

Generating Mucosal-Like Mast Cells from Bone Marrow

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To obtain bone marrow derived mucosal-like mast cells (BM-MMCs), bone marrow cells was collected from femur and tibia. The cells were washed two times in PBS and cultured in complete Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% FCS, 1% PEST, 2 mM L-glutamine, 5ng/ml mouse interleukin (IL) -9 (ImmunoTools), 1ng/ml recombinant human transforming growth factor beta (TGF-beta, ImmunoTools), 1ng/ml mouse IL-3 (ImmunoTools) and 50ng/ml mouse stem cell factor (SCF, ImmunoTools). After two weeks >99% of the cells showed BM-MMCs characteristics as verified by May-Grünwald/Giemsa staining. The BM-MMCs were washed three times in PBS and seeded in duplicates (first experiment) or triplicates (second experiment) at 2x10 6 BM-MMCs/ml in HBSS and challenged with different concentrations (25 ng/ml, 100 ng/ml and 1μg/ml) of soluble protein extracts from three different Giardia isolates (GS, WB and H3). After 6h or 24h incubation (at 37 0 C, 5% CO 2 ), supernatants were collected and frozen at -20 0 C until used.
Li Z., Peirasmaki D., Svärd S, & Åbrink M. (2019). Giardia excretory-secretory proteins modulate the enzymatic activities of mast cell chymase and tryptase. Molecular immunology, 114.
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