Human umbilical vein endothelial cells (HUVECs) were isolated from human umbilical cords. HUVECs were cultured in an Endo GRO-VEGF Complete Culture Media Kit (SCME002, Millipore) at 37°C in a humidified atmosphere of 5% CO2. HEK293T cells were obtained from the American Type Culture Collection (ATCC). HEK293T cells were cultured in DMEM (11965092, Gibco) with 10% fetal bovine serum (FBS) (1600044, Gibco) at 37°C in a humidified atmosphere of 5% CO2.
Endo gro vegf complete culture media kit
Manufactured by Merck Group
The Endo GRO-VEGF Complete Culture Media Kit is a laboratory product designed to provide a comprehensive solution for the in vitro culture and maintenance of endothelial cells. The kit includes essential growth factors, supplements, and other components required for the optimal growth and proliferation of endothelial cells in cell culture applications.
Automatically generated - may contain errors
Lab products found in correlation
Hek293t cells, by Thermo Fisher Scientific (1 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Hek293t cell, by American Type Culture Collection (1 mentions)
Huvecs, by Merck Group (1 mentions)
2 protocols using endo gro vegf complete culture media kit
1
Isolation and Culture of HUVECs and HEK293T Cells
2
Culturing Human Umbilical Vein Endothelial Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
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HUVECs (pooled donor, LONZA, Allendale,
New Jersey) were grown to 70–80% confluence for each experiment
(P2–P4) in a T-75 cell culture flask using complete media (EndoGRO-VEGF
Complete Culture Media Kit, Millipore, Billerica, MA) supplemented
with 1% penicillin–streptomycin. Media were changed every 3
days.
New Jersey) were grown to 70–80% confluence for each experiment
(P2–P4) in a T-75 cell culture flask using complete media (EndoGRO-VEGF
Complete Culture Media Kit, Millipore, Billerica, MA) supplemented
with 1% penicillin–streptomycin. Media were changed every 3
days.
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