Four well glass culture slides

Manufactured by BD

Sourced in United States

The four-well glass culture slides are a laboratory equipment designed to facilitate the culturing and observation of cells or tissues. These slides provide a contained environment with four separate wells, allowing for the simultaneous culture and analysis of multiple samples. The slides are made of glass, providing a transparent surface for microscopic observation. The core function of these slides is to serve as a platform for cell culture and related experimental activities.

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Lab products found in correlation

Lipofectamine 3000, by Thermo Fisher Scientific (1 mentions) Mda mb 231, by American Type Culture Collection (1 mentions) Streptomycin, by Thermo Fisher Scientific (1 mentions) L glutamine, by Thermo Fisher Scientific (1 mentions) Penicillin, by Thermo Fisher Scientific (1 mentions) Recombinant human egf, by Thermo Fisher Scientific (1 mentions) Axio imager m2 microscope, by Zeiss (1 mentions) On targetplus sirna, by Horizon Discovery (1 mentions) Axiovision software, by Zeiss (1 mentions) Anti phospho fak y397, by Abcam (1 mentions)

Spelling variants of this product

Culture slides (24 citations) Glass culture slides (7 citations) Glass slide (7 citations) Four-well culture slides (6 citations)

2 protocols using four well glass culture slides

Cell Culture and Transfection Protocol

Cited 1 time

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HeLa and MDA‐MB‐231 cells (American Type Culture Collection (ATCC), Manassas, USA) were grown in DMEM supplemented with 10% FCS, 1% penicillin (10,000 U/ml)/streptomycin (10,000 µg/ml), and 1% l‐glutamine (200 mM) (Thermo Fisher Scientific, Waltham, USA). Cells lines were grown at 37°C in a 5% CO₂ humidified incubator.
Cells were transfected with Lipofectamine 3000 (HeLa; Thermo Fisher Scientific, Waltham, USA) or Xtreme‐GENE HP ^TM (MDA‐MB‐231; Roche Diagnostics, Indianapolis, USA) transfection reagents according to manufacturer’s instructions. Cells were seeded between 0.5 × 10⁶ and 1.0 × 10⁶ cells for cell cycle analysis or protein experiments, or 0.7–2.5 × 10⁴ on four‐well glass culture slides (BD Falcon, Bedford, USA) for microscopy experiments. The cells were transfected with 2 µg of plasmid DNA for protein experiments and biological assays, or 0.25 µg of plasmid DNA for confocal microscopy experiments. For those experiments with EGF stimulation, cells were rested in EBSS (Thermo Fisher Scientific, Waltham, USA) medium without serum and stimulated with 100 ng/ml recombinant human EGF (Thermo Fisher Scientific, Waltham, USA) for the indicated times as had been done previously (Dykes et al, 2017 (link)).

Lin J., McCann A.P., Sereesongsaeng N., Burden J.M., Alsa’d A.A., Burden R.E., Micu I., Williams R., Van Schaeybroeck S., Evergren E., Mullan P., Simpson J.C., Scott C.J, & Burrows J.F. (2022). USP17 is required for peripheral trafficking of lysosomes. EMBO Reports, 23(4), e51932.

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TGF-β Modulates FAK Activation in Cardiac Fibroblasts

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In order to assess focal adhesion kinase (FAK) activation, cardiac fibroblasts (1*10⁵ cells per well) from wild type mice were seeded on four-well glass culture slides (BD Falcon). Two days later, cells were serum-starved for 16 hours before adding different doses of TGF-β for 65 min. The cells were fixed for 30 min with 4% paraformaldehyde and processed for immunofluorescence with antibodies recognizing phosphorylated FAK (Anti-phospho Y397 FAK, #ab39967, Abcam) and α-SMA (1A4, Santa Cruz Biotech), followed by Alexa Fluor 488– or Alexa Fluor 597–conjugated secondary antibodies respectively. The slides were mounted using VECTASHIELD Antifade Mounting Medium with DAPI (#H-1200). Images were obtained with a Zeiss Axio Imager.M2 microscope and Zeiss AxioVision software.
For the α-SMA knockdown, cardiac fibroblasts from wild type mice were transfected with 50 nM ONTARGETplus siRNA to α-SMA or transfected with a non-silencing control siRNA (Dharmacon) and for overexpression experiments, cells were transfected with 2.5 ng of α-SMA cDNA (Origene TM Technologies) or transfected with a control entry vector using Lipofectamine®3000 Reagent. The cells were returned to a 5% CO2 incubator, allowed to recover for 72 hours, then fixed for 30 min with 4% paraformaldehyde and processed for immunofluorescence with the anti-α-SMA antibody.

Shinde A.V., Humeres C, & Frangogiannis N.G. (2016). The role of α-smooth muscle actin in fibroblast-mediated matrix contraction and remodeling. Biochimica et biophysica acta, 1863(1), 298-309.

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