Dmem culture media

Manufactured by Corning

Sourced in United States

DMEM (Dulbecco's Modified Eagle Medium) is a cell culture medium commonly used to support the growth and maintenance of various cell types in laboratory settings. It is a basal medium that provides the necessary nutrients, vitamins, and other components required for cell proliferation and survival. DMEM is designed to maintain the optimal pH and osmotic balance for cell cultures.

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Lab products found in correlation

Protein a g bead, by Santa Cruz Biotechnology (2 mentions) Pvdf membrane, by Bio-Rad (2 mentions) Dulbecco phosphate buffered saline (dpbs), by Corning (2 mentions) Co2 incubator, by Sanyo (1 mentions) 35s met cys, by PerkinElmer (1 mentions)

Close spelling variants of this product

DMEM media (79 citations) Culture media (2 citations)

4 protocols using dmem culture media

Quantitative Capsule Visualization

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Capsule was induced based on the original protocol described by Granger D.L. et al. (75 (link), 76 (link)). Overnight cultures were washed twice with PBS, resuspended in the DMEM culture media (Corning, Corning, NY) with addition of 10% FBS (Neuromics, Edina, MN), and transferred to 6-well plates with 10⁶ cells in each well. Cells were incubated for 5 days at 37°C with 5% CO₂, stained with India ink, and then observed under the Leica DMi8 inverted fluorescence microscope with a 100× objective. The size of the capsule was quantified using ImageJ software by measuring the width of the halo created by the capsule, which is visualized by negative staining with India ink.

Stempinski P.R., Goughenour K.D., du Plooy L.M., Alspaugh J.A., Olszewski M.A, & Kozubowski L. (2022). The Cryptococcus neoformans Flc1 Homologue Controls Calcium Homeostasis and Confers Fungal Pathogenicity in the Infected Hosts. mBio, 13(5), e02253-22.

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Culturing Human Cancer Cell Lines

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We used cell lines of human fibrosarcoma (cell culture HT-1080) and breast cancer (MCF-7) obtained at the Research Institute of Virology (D. I. Ivanovsky Institute of Virology, Moscow, Russia). Tumor cells were cultured according to the recommendations specified in the cell culture certificates. Cells were incubated in DMEM culture media supplemented with 2 mM L-glutamine and 10% FBS in 25 cm² and 75 cm² culture flasks (Corning, Glendale, AZ, USA) at 37 °C in a humid atmosphere with 5% CO₂ in a Sanyo CO₂ incubator (Sanyo, Moriguchi, Japan). Cell lines were used in the study after 3 to 10 passages.

Kostryukova L.V., Tereshkina Y.A., Tikhonova E.G., Khudoklinova Y.Y., Bobrova D.V., Gisina A.M., Morozevich G.E., Pronina V.V., Bulko T.V, & Shumyantseva V.V. (2023). Effect of an NGR Peptide on the Efficacy of the Doxorubicin Phospholipid Delivery System. Nanomaterials, 13(15), 2229.

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Pulse-Chase Analysis of Protein Turnover

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For Pulse-Chase experiments, COS7 cells were incubated with Met/Cys-deficient DMEM (Corning) with 10% FBS for 30 min at 37 °C. The cells were then pulse-labeled with 0.5 mCi/ml [³⁵S]Met/Cys (Perkin Elmer) in Met/Cys-deficient DMEM for 7 min at 37 °C, media was removed, cells were washed with dPBS (Corning), and cells were chased with DMEM culture media (Corning) containing 200 mM unlabeled methionine and 200 mM unlabeled cysteine and 10% FBS. At the indicated times, cells were lysed in PPHB and centrifuged at 13,000 x g for three min at 22 °C, supernatants were pre-cleared for 30 min at 4 °C with Protein A/G Beads (Santa Cruz), and protein complexes were immunoprecipitated from the supernatant with 1:100 dilution of either an anti-HA or anti-FLAG monoclonal antibody (BioLegend, 16B12; BioLegend L5) overnight at 4°C. The immune complexes were collected with Protein A/G Beads (Santa Cruz) for 2 h at 4 °C, washed 4 times with PPHB, separated by SDS-PAGE, transferred to PVDF membrane (Biorad), and analyzed by autoradiography and western blot. GST pull-down experiments utilized a similar approach with Glutathione Resin (Pierce; 16101) for purification.

Baffi T.R., Lordén G., Wozniak J.M., Feichtner A., Yeung W., Kornev A.P., King C.C., Del Rio J.C., Limaye A.J., Bogomolovas J., Gould C.M., Chen J., Kennedy E.J., Kannan N., Gonzalez D.J., Stefan E., Taylor S.S, & Newton A.C. (2021). mTORC2 Controls PKC and Akt by Phosphorylating a Conserved TOR-Interaction Motif. Science signaling, 14(678), eabe4509.

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Pulse-Chase Protein Interaction Assay

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For pulse-chase experiments, COS7 cells were incubated with Met/Cys-deficient DMEM for 30 min at 37 °C. The cells were then pulse-labeled with 0.5 mCi/ml [³⁵S]Met/Cys in Met/Cys-deficient DMEM for 7 min at 37 °C, media were removed, washed with dPBS (Corning), and chased with DMEM culture media (Corning) containing 200 mM unlabeled methionine and 200 mM unlabeled cysteine. At the indicated times, cells were lysed in PPHB and centrifuged at 13,000 x g for 3 min at 22 °C, supernatants were pre-cleared for 30 min at 4 °C with Protein A/G Beads (Santa Cruz), and protein complexes were immunoprecipitated from the supernatant with either an anti-HA or anti-FLAG monoclonal antibody (BioLegend, 16B12; BioLegend L5) overnight at 4 °C. The immune complexes were collected with Protein A/G Beads (Santa Cruz) for 2 hrs, washed 3x with PPHB, separated by SDS-PAGE, transferred to PVDF membrane (Biorad), and analyzed by autoradiography and western blot. Co-immunoprecipitation experiments were performed similarly, omitting the labeling and autoradiography steps.

Baffi T.R., Van A.A., Zhao W., Mills G.B, & Newton A.C. (2019). Protein Kinase C Quality Control by Phosphatase PHLPP1 Unveils Loss-of-Function Mechanism in Cancer. Molecular cell, 74(2), 378-392.e5.

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