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3 protocols using 1 1 diphenyl 2 picrylhydrazyl dpph 2 2 azino bis 3 ethylbenzothiazoline 6 sulfonic acid diammonium salt abts

1

Antioxidant and Antimicrobial Evaluation

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Standard D-(-)-quinic acid, L-(-)-malic acid, citric acid, chlorogenic acid, Folin–Ciocalteu's phenol reagent, 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) were obtained from Sigma-Aldrich (Saint Louis, MO, USA). Methanol (high performance liquid chromatography grade) acetonitrile (LC-MS reagent) and water (LC-MS grade) were purchased from RCI Labscan Limited (Bangkok, Thailand). Formic acid (analytical grade) was purchased from JT Baker (Philipsburg, NJ, USA). A nylon syringe filter 0.45 μm was bought from Lubitech Technologies Ltd. (Shanghai, China). Muller-Hinton agar (MHA) and Muller-Hinton broth (MHB) were purchased from Difco Laboratories, Inc. (Difco, Franklin Lakes, NJ, USA).
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2

Camellia Species Seed Maturation Analysis

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A total of 40 Camellia spp. samples in seed maturation were provided by Prof. Kaibing Zhou in 2018 for this study, including 26 C. vietnamensis Huang varieties, 7 C. oleifera Abel., 2 C. osmantha, 1 C. gigantocarpa Hu et Huang, 1 C. chekiangoleosa, 1 C. gauchowensis, 1 C. fangchengensis S.Y. Liang et Y.C. Zheng, and 1 C. sinensis (L.) O. Ktze. Sample numbers are shown in Table 1.
Squalene, vitamin E, stigmasterol, β-amyrin, 3,4-dihydroxyphenylacetic acid, epicatechin, tea saponin, rutin, quercetin, camellianin A standards, 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS), and Trolox were purchased from Sigma–Aldrich Chemical Co. Ltd. (Shanghai, China). Other reagents and solvents were obtained from Shanghai Macklin Biochemical Co. Ltd. (Shanghai, China).
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3

Antioxidant Activity Evaluation Methods

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Folin–Ciocalteu, trolox, gallic acid, 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2′-azino-bis (3-ethyl-benzothiazoline-6-sulfonic acid) diammonium salt (ABTS), nitroblue tetrazolium (NBT) chloride, N-methylphenazonium methyl sulfate (PMS), nicotinamide adenine dinucleotide (NADH) were purchased from Sigma Chemical Co. (St. Louis, MO, USA). Other analytical grades of methanol, ethanol, H2O2, FeSO4, K2S2O8 and salicylic acid were purchased from Tianjin Kemio Chemical Co. (Tianjin, China).
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