The largest database of trusted experimental protocols

9 protocols using aluminum foil

1

Electrochemical Characterization of Aluminum

Check if the same lab product or an alternative is used in the 5 most similar protocols
Aluminum foil (Al, product number: 326860-3.6G), glucose (C6H12O6, product number: PHR1000-1G), magnesium chloride (MgCl2, product number 449172-10G), oxalic acid (C2H2O4, product number: 247537-500G), perchloric acid (HClO4, product number: 311421-250ML), phosphoric acid (H3PO4, product number: 695017-500ML), sodium hydroxide (NaOH, product number: 306576-100G), and deionized water (H2O, product number: W4502-1L with a resistivity of 18 MΩ) were procured from Sigma-Aldrich (St. Louis, MI, USA). These reagents were employed as provided, without any further purification, ensuring the consistency of experimental conditions.
+ Open protocol
+ Expand
2

Fabrication of Flexible FeS2@C/CNT Film

Check if the same lab product or an alternative is used in the 5 most similar protocols
The flexible FeS2@C/CNT film was fabricated by the vacuum filtration method. Firstly, 15 mg of CNT and 35 mg FeS2@C were dispersed in 80 mL H2O using an intensive ultrasonication probe for 30 min. Then, the mixed solution was filtered through a mixed cellulose ester membrane (1.2 µm pore size, Millipore). The obtained filter cake was then vacuum-dried for 24 h to get a freestanding film.
The binder-free and freestanding FeS2@C/CNT directly utilized as a cathode in the AIBs. To compare with commercial FeS2, the FeS2 was firstly mixed with KB carbon with the same carbon content in FeS2@C/CNT. Then the mixture and polytetrafluoroethylene (PTFE) (weight ratio of 9:1) mixed in deionized water followed by overnight high-vacuum heating under 100 °C. The areal loading of the active materials is around 1.0 mg cm−2. The 1-ethyl-3-methylimidazolium chloride ([EMIm]Cl, 98%, Sigma) mixed with anhydrous aluminum chloride (99.99%, Sigma-Aldrich) (mole ratio of 1.3) to obtain the ionic liquid (IL) electrolyte (35 μL). A piece of glass fiber was used as the separator (Whatman). The aluminum foil (Sigma, 99.999%, 0.25 mm) is directly treated as the anode electrode. We applied soft package and Swagelok-type cells to assemble the batteries in glove box filled with Ar gas.
+ Open protocol
+ Expand
3

Osteoblast Differentiation and Mineralization

Check if the same lab product or an alternative is used in the 5 most similar protocols
For ALP staining, differentiated osteoblasts were washed with PBS and fixed in 10% formalin for 2 min. Cells were rinsed with deionized water and incubated with alkaline dye mixture for 30 min by wrapping with aluminum foil according to the manufacturer’s instruction (Sigma-Aldrich). Cells were then rinsed with deionized water and subjected to a microscopic observation. For alizarin red S staining, cells were washed with distilled water, fixed in ice-cold 70% ethanol for 1 h, and then stained with 2% Alizarin red S stain solution (adjusted to pH 4.2 with 10% ammonium hydroxide) (Sigma-Aldrich) for 30 min. Stained cells were washed and observed using a light microscope. At least 10 cell images were captured under a microscope (Olympus IX51 inverted fluorescent microscope, Olympus Optical, Japan) fitted with a DP70 camera system (Olympus) and used for a quantitative analysis with the Image J software. To determine ALP activity, cells were washed and lysed with Triton X-100 lysis buffer (50 mM Tris, 150 mM NaCl, and 1% Triton X-100, pH 10). Cell supernatants were incubated with an ALP substrate. ALP activity assay was performed in triplicate using the LabAssay ALP Kit (Wako Pure Chemical Industries, Japan). Relative ALP activity was calculated by comparing with standard solution and expressed as units per mg of protein according to the manufacturer’s instructions.
+ Open protocol
+ Expand
4

TLC-based Identification and Quantification of PCSEE and BIO-A

Check if the same lab product or an alternative is used in the 5 most similar protocols
PCSEE and BIO-A were concurrently run on a silica gel matrix (5 × 10 cm2) containing a fluorescent indicator with 254 nm excitation on aluminum foil (Sigma-Aldrich) for thin-layer chromatography (TLC) identification. On replicate TLC matrices, 5 μL of a 1 cm short band of 20 mg/mL PCSEE, and 1 and 5 mM BIO-A (Sigma-Aldrich) ethanol stock solution were directly deposited at 0.5 cm or more from TLC plate lower edge. After ethanol evaporation, TLC plate was slowly placed and developed in a clean glass developing chamber saturated with a miscible solvent system (water: acetone: ethanol of 5: 3: 1 ratio), 0.5 cm deep or less. Plates were taken out after 50-60 min incubation at RT, or when solvent front reached 1 cm from the upper TLC edge (approximately 8.5 cm height). Dried developed TLC plates were then visualized under short (254 nm) and long (366 nm) UV lights. Retention factor (Rf) of BIO-A band and a matching band were quantified.
+ Open protocol
+ Expand
5

Polycaprolactone-based Biomaterial Characterization

Check if the same lab product or an alternative is used in the 5 most similar protocols
Polycaprolactone (PCL, IUPAC name: (1,7)-Polyoxepan-2-one, CAS: 24980-41-4, Mn ~80 kDa, Cat.No: 440744, St. Louis, Missouri, USA), Toluidine Blue O (TBO, Mw: 305.83 g/mol, CAS: 92-31-9, St. Louis, Missouri, USA), Dulbecco’s Phosphate Buffered Saline (PBS, Lot No. RNBH1585, St. Louis, Missouri, USA), Sodium dodecyl sulfate (SDS, ≥98.5%, CAS: 151-21-3, St. Louis, Missouri, USA), and aluminum foil (Lot No. 515981, St. Louis, Missouri, USA) were purchased from Sigma-Aldrich Corp., St. Louis, Missouri, USA. Acetone (HPLC grade, CAS: 67-64-1), N.N-Dimethylformamide (DMF, HPLC grade, CAS: 68-12-2) and Sodium hydroxide solution (NaOH, 0.1 N, CAS N 1310-73-2) were purchased from Eurochemicals (Spa, Milan, Italy).
+ Open protocol
+ Expand
6

Chromatographic Analysis of Saccharide Hydrolysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Mono- and oligosaccharide composition of the hydrolysis and transglycosylation products were analyzed on silica gel TLC plates on aluminum foil (Sigma-Aldrich, St. Louis, MO, USA) with a 254 nm fluorescent indicator. The pore diameter was 60 A. Rf was calculated for every stain.
Weighed freeze-dried reaction product mixtures were placed in 0.5 mL Eppendorf, dissolved in distilled water to a concentration of 5 mg/mL, and centrifuged at 10,000 rpm to remove the denatured protein. A small spot of the analyzed mixture and standard compounds were applied at the start line of the TLC plate and chromatographed over 30 minutes in a sealed chamber Latch-Lid ChromatoTank (General Glass Blowing Co. Inc., Richmond, CA, USA), containing 100 mL of the mobile phase butanol/acetic acid/water (3:1:1; v/v/v). For visualization of stains, the plate was treated three times with 5% sulfuric acid solution, drying by warm air after each spraying.
+ Open protocol
+ Expand
7

Synthesis and Drug Loading of Stöber Silica Nanoparticles

Check if the same lab product or an alternative is used in the 5 most similar protocols
Materials. Tetraethyl orthosilicate (TEOS, 99.9%), ammonium hydroxide (NH4OH, 28%), magnesium -325 mesh powder (Mg, 99.8%), and aluminum powder (Al, 99.5%) were purchased from Alfa Aesar. Calcium (Ca, 99%), phosphate buffered saline (pH 7.4), ibuprofen (≥ 98%), magnesium chips (Mg, 99.98%), magnesium foil (Mg, 99.9%), aluminum granules (Al, 99.7%), aluminum foil (Al, 98%), dialysis sacks and phosphoric acid solution (H3PO4, 85%) were purchased from Sigma-Aldrich. Sodium chloride (NaCl, 99%) was purchased from Chimiques ACP Chemicals Inc. Hydrochloric acid (HCl, 36.5-38.0%) was purchased from Anachemia. Water (≥18.2 MΩ cm resistivity) was obtained from a Barnstead E-Pure system. All the reagents were used as received without further purification.
Synthesis of Stӧber silica NPs. 3.00 mL of 28% NH4OH was added to 50.0 mL of 95% EtOH and stirred together for 5 min, followed by the addition of 1.50 mL of TEOS. The reaction was stirred at 18 °C for 12 h (ambient humidity was recorded to be 82%). The silica product was collected by centrifuging at 3300 rpm for 15 min. The solid white product was washed three times with EtOH and dried overnight in an oven at 80 °C. Drug loading and release. Si-NPs (25 mg) were treated with 10 mL of 49% hydrofluoric acid solution for 15 min and collected via centrifugation.
+ Open protocol
+ Expand
8

Salivette-based Saliva Collection for SERS Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Saliva collection was carried out using Salivette swabs (Sarstedt, Germany), following manufacturer’s instructions. Briefly, the swab was placed in the subject’s mouth and chewed for 60 s to stimulate salivation. The cotton swab was then closed into the provided plastic tube and centrifuged for 2 min at 1000g in order to remove eventual food debris and to collect saliva. Saliva samples were stored at − 20 °C. To limit the variability in biomolecules concentration in saliva, the collection procedure was performed at fixed time-points after breeding and teeth brushing. Pre-analytical parameters, including collection time, time between the collection of saliva and the Raman analysis and storage temperature, were recorded. All the data were anonymized preserving the subject privacy. A saliva drop (3 µl) was deposited on a glass slide covered with commercially available Aluminum foil, in order to achieve the SERS effect, and dried at room temperature for 10–15 min. Aluminum foil was purchased from Merck KGaA (Germany) and used as received to cover the glass substrate for the SERS analysis.
+ Open protocol
+ Expand
9

Analytical Characterization of Compounds

Check if the same lab product or an alternative is used in the 5 most similar protocols
Mass spectra were characterized using a Shimadzu gas chromatography–mass spectrometry (GC-MS) QP2010 Ultra. The 1D nuclear magnetic resonance (1 H-NMR) spectrum was measured in a CDCl3 solvent with 400 MHz JEOL spectrophotometer. The infrared (IR) spectra were recorded on PerkinElmer Fourier transform-IR spectrophotometer, using a KBr disc in the range 4000–400 cm−1. Column chromatography was performed on silica gel G60 (70–230 mesh Merck). Thin-layer chromatography (TLC) analysis was carried out using precoated silica gel G60-F254 on aluminum foil (Merck).
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!