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Mouse anti mll1

Manufactured by Merck Group
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Mouse anti-MLL1 is a laboratory reagent used for the detection and analysis of the MLL1 protein. It is a monoclonal antibody produced in mice that specifically binds to the MLL1 protein. MLL1 is a histone methyltransferase that plays a crucial role in regulating gene expression during development and hematopoiesis.

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Lab products found in correlation

Rabbit anti hoxa9 07 178, by Merck Group (2 mentions) Sc 304, by Santa Cruz Biotechnology (2 mentions) Alexa fluor 488 anti rabbit igg, by Thermo Fisher Scientific (2 mentions) Anti mouse igg alexa fluor 594, by Thermo Fisher Scientific (2 mentions) Anti mouse igg1 alexa fluor 594, by Thermo Fisher Scientific (2 mentions)

Close spelling variants of this product

Anti-MLL1 (2 citations)

2 protocols using mouse anti mll1

1

Immunofluorescence Analysis of Satellite Cells

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Freshly isolated SCs were allowed to settle on poly-L-lysine coated diagnostic microscope slides for 30 min at RT. All cells and myofibers were fixed with 2% PFA/PBS, permeabilized with 0.5% Triton X-100/PBS and blocked with 10 % Horse Serum/PBS for 1h at RT. Cells and fibers were stained with primary antibodies in blocking solution overnight at 4°C. Samples were washed three times with PBS and incubated with secondary antibodies for 1h at RT. Nuclei were counterstained with DAPI/PBS. Cultured cells were kept in PBS; freshly isolated SCs and myofibers were mounted with Permaflour. The following primary antibodies were used: undiluted mouse anti-Pax7 (DSHB), 1:300 rabbit anti-Hoxa9 (07-178, Millipore), 1:500 mouse anti-MLL1 (05-765, Millipore), 1:500 rabbit anti-WDR5 (A302-429A, Bethyl Laboratories), 1:300 rabbit anti-H3K4me3 (C15410003-50, Diagenode), 1:200 rabbit anti-MyoD (sc-304, Santa Cruz). The following secondary antibodies were used at 1:1,000 concentration: anti-rabbit IgG Alexa-Fluor 488, anti-mouse IgG Alexa-Fluor 594, anti-mouse IgG1 Alexa-Fluor 594 (Life Technologies).
Schwoerer S., Becker F., Feller C., Baig A.H., Koeber U., Henze H., Kraus J.M., Xin B., Lechel A., Lipka D.B., Varghese C.S., Schmidt M., Rohs R., Aebersold R., Medina K.L., Kestler H.A., Neri F., von Maltzahn J., Tuempel S, & Rudolph K.L. (2016). Epigenetic stress responses induce muscle stem cell aging by Hoxa9 developmental signals. Nature, 540(7633), 428-432.
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2

Immunofluorescence Analysis of Satellite Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Freshly isolated SCs were allowed to settle on poly-L-lysine coated diagnostic microscope slides for 30 min at RT. All cells and myofibers were fixed with 2% PFA/PBS, permeabilized with 0.5% Triton X-100/PBS and blocked with 10 % Horse Serum/PBS for 1h at RT. Cells and fibers were stained with primary antibodies in blocking solution overnight at 4°C. Samples were washed three times with PBS and incubated with secondary antibodies for 1h at RT. Nuclei were counterstained with DAPI/PBS. Cultured cells were kept in PBS; freshly isolated SCs and myofibers were mounted with Permaflour. The following primary antibodies were used: undiluted mouse anti-Pax7 (DSHB), 1:300 rabbit anti-Hoxa9 (07-178, Millipore), 1:500 mouse anti-MLL1 (05-765, Millipore), 1:500 rabbit anti-WDR5 (A302-429A, Bethyl Laboratories), 1:300 rabbit anti-H3K4me3 (C15410003-50, Diagenode), 1:200 rabbit anti-MyoD (sc-304, Santa Cruz). The following secondary antibodies were used at 1:1,000 concentration: anti-rabbit IgG Alexa-Fluor 488, anti-mouse IgG Alexa-Fluor 594, anti-mouse IgG1 Alexa-Fluor 594 (Life Technologies).
Schwoerer S., Becker F., Feller C., Baig A.H., Koeber U., Henze H., Kraus J.M., Xin B., Lechel A., Lipka D.B., Varghese C.S., Schmidt M., Rohs R., Aebersold R., Medina K.L., Kestler H.A., Neri F., von Maltzahn J., Tuempel S, & Rudolph K.L. (2016). Epigenetic stress responses induce muscle stem cell aging by Hoxa9 developmental signals. Nature, 540(7633), 428-432.
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