Recombinant mouse‐soluble M‐CSF and RANKL were purchased from PeproTech (Rocky Hill, NJ, USA). Lipofectamine 2000 reagent and TRIZOL reagent were from Life Technologies (Carlsbad, CA, USA). The PrimeScript RT Reagent Kit was obtained from Takara Biotechnology (Shiga, Japan). Fast SYBR Green Master Mix and PowerUp SYBR Green Master Mix were from Thermo Fisher Scientific (Waltham, MA, USA). DCFH‐DA (2′,7′‐dichlorofluorescein diacetate) powder was from Sigma‐Aldrich (St. Louis, MO, USA), and Tempol (4‐hydroxy‐2,2,6,6‐tetramethylpiperidinyloxy) was purchased from Selleckchem (Houston, TX, USA). RAW264.7 macrophages were obtained from the Cell Center of Peking Union Medical College.
Tempol
Manufactured by Selleck Chemicals
Sourced in United States
Tempol is a stable nitroxide radical compound that functions as a redox-active antioxidant and free radical scavenger. It is commonly used in research applications involving oxidative stress and related biological processes.
Automatically generated - may contain errors
Lab products found in correlation
Powerup sybr green master mix, by Thermo Fisher Scientific (1 mentions)
Trizol reagent, by Thermo Fisher Scientific (1 mentions)
Primescript rt reagent kit, by Takara Bio (1 mentions)
Rankl, by Thermo Fisher Scientific (1 mentions)
Lipofectamine 2000 reagent, by Thermo Fisher Scientific (1 mentions)
Fast sybr green master mix, by Thermo Fisher Scientific (1 mentions)
β actin, by Cell Signaling Technology (1 mentions)
Antibodies against cleaved caspase 3, by Cell Signaling Technology (1 mentions)
Sybr premix ex taq 2, by Takara Bio (1 mentions)
Nf κb p65, by Abcam (1 mentions)
Fugene hd transfection reagent, by Promega (1 mentions)
Control pcdna3.1 plasmid, by GenePharma (1 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions)
Trizol, by Thermo Fisher Scientific (1 mentions)
E cadherin, by Proteintech (1 mentions)
Interleukin 6 (il 6), by Proteintech (1 mentions)
Adeno associated virus serotype 9 aav9, by Hanbio Biotechnology (1 mentions)
Tgf β1, by Abcam (1 mentions)
β actin, by Proteintech (1 mentions)
Dmem f12 medium, by Thermo Fisher Scientific (1 mentions)
3 protocols using tempol
1
Osteoclastogenesis Regulation via ROS
2
Oxidative Stress Assessment Protocol
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Antibodies against Cleaved Caspase-3, SOD1, SOD2 and β-actin were purchased from Cell Signaling Technology (#9661, #37385, #13141, #12262, Danvers, MA, USA). Tempol was purchased from Selleck Chemicals LLC (#S2910, Houston, TX, USA). Kits for terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining, total antioxidant capacity (T-AOC), malonyldialdehyde (MDA) and SOD activity assay were purchased from Beyotime Institute of Biotechnology (#C1091, #S0119, #S0131S, #S0101, Shanghai, China). 3-nitrotyrosine (3′-NT) and advanced glycation end products (AGEs) ELISA kits were purchased BlueGene Biotech (#E02A0670, #E02A002 Shanghai, China). Wright's stain kit was obtained from Coolaber Biotechnology Co. Ltd (#SL7030, Beijing, China). All other chemicals made in China were of analytical grade.
3
Antibodies and Reagents for Cell Culture
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2.1 Antibodies and other reagents DMEM, DMEM-F12 medium and fetal bovine serum (FBS) was from Gibco (Gaithersburg, Maryland). Penicillin and streptomycin were purchased from Invitgen (Carlsbad, CA). N-acetylcysteine (NAC), mannitol came from Sigma (St. Louis, MO). Tempol and TAK242 came from Selleck Chemicals (Houston, Texas). NQO1 pcDNA3.1(+) plasmid and control pcDNA3.1(+) plasmid were bought from GenePharma (Suzhou, China). FuGENEHD transfection reagents were obtained from Promega (Madison, Wisconsin, USA). TRIzol was bought from Invitrogen (Carlsbad, CA). Takara (Shiga, Japan) was the source of SYBR Premix Ex Taq II. The antibodies against α-smooth muscle actin (α-SMA), E-cadherin, collagen IV, bronectin, Nox1, Nox4, TLR4, NQO1, Histone H3, IL-6, TNF-α and β-actin were bought from Proteintech (Chicago, IL), while the antibodies of TGF-β1, NF-κB p65 and MCP-1 came from Abcam (Cambridge, UK). Antibodies against Smad-2, Smad-3, P-Smad2, P-Smad3 were purchased from Cell Signaling Technology (Beverly, MS). Adeno-associated virus serotype 9 (AAV9) was obtained from HanBio Technology (HH20191024HBYXL-AAV01, Shanghai, China).
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