Recombinant human sil 6r

Antibodies against IL-6 (R-49L), IL-6R (C-20) and total STAT3 (C-20) were from Santa Cruz Biotechnology (Santa Cruz, CA). Recombinant human IL-6 was obtained from Chemicon (Temecula, CA) and recombinant human sIL-6R was from R&D systems (Minneapolis, MN). Antibodies against total-p44/42 MAPK (ERK 1/2) (3A7), phosphorylated (Thr202/Tyr204) p44/42 MAPK (p-ERK 1/2) (E10), phosphorylated (Tyr705) STAT3 (p-STAT3) and β-actin were from Cell Signaling (Beverly, MA). Humanized anti-human IL-6 receptor antibody, tocilizumab, was kindly provided by Chugai Pharmaceutical Co. Ltd. (Shizuoka, Japan) and non-immune human IgG was purchased from Jackson Immuno Research (West Grove, PA). Antibody against CD68 (25747–1-AP) was purchased from Proteintech group Inc. (Chicago, IL, USA). Growth factor-reduced basement membrane proteins (Matrigel) and 24-transwell chambers were purchased from BD Biosciences (Bedford, MA).

Recombinant human sIL-6R and goat anti-IL-6 were purchased from R&D Systems. The mouse anti-STAT3, rabbit anti-phosphor-STAT3 (Tyr705) (pSTAT3), and horseradish peroxidase (HRP)-linked anti-rabbit and anti-mouse antibodies were from Cell Signaling Technology, and recombinant human IL-6 and mouse anti-IL-6R (B-R6) were from Millipore. Rabbit anti-IL-6R (C-20) was obtained from Santa Cruz Biotechnology, mouse anti-cd49b was from BD Biosciences, mouse anti-LAMP-1 (H4A3) was from the Developmental Studies Hybridoma Bank, and NT was from Sigma. The murine monoclonal anti-SorLA (31 (link)) and the rabbit polyclonal anti-SorLA have previously been described (32 (link)). Secondary antibodies conjugated with Alexa Fluor dyes were all from Invitrogen.