Toluene and acetonitrile (LC-MS grade) were purchased from Merck (Darmstadt, Germany), and formic acid was from Sigma-Aldrich (St Louis, MO, USA). β-Glucuronidase (Escherichia coli) was obtained from Roche Diagnostics (Mannheim, Germany). Water was produced by the Milli-Q Integral 5 system, Millipore (Billerica, MA, USA). The analytical standard 8-oxodG was purchased from Sigma-Aldrich, and 15N5-8-oxodG was from Cambridge Isotope Laboratories Inc. (Tewksbury, MA, USA). All other chemical standards and deuterium-labeled internal standards were purchased from Toronto Research Chemicals (Toronto, ON, Canada). NaCl (>99.5%) was purchased from Sigma-Aldrich and ultrapure water was from VWR Chemicals (Darmstadt, Germany).
Ultrapure water
Manufactured by Avantor
Sourced in United States
Ultrapure water is a highly purified form of water used in various laboratory applications. It is produced through a multi-stage purification process, typically involving deionization, reverse osmosis, and other techniques to remove impurities and contaminants. Ultrapure water is characterized by its extremely low levels of dissolved ions, organic matter, and microorganisms, making it suitable for use in sensitive analytical procedures, reagent preparation, and other critical laboratory workflows.
Automatically generated - may contain errors
Lab products found in correlation
8 oxodg, by Merck Group (1 mentions)
Lc ms grade acetonitrile, by Merck Group (1 mentions)
β glucuronidase escherichia coli, by Roche (1 mentions)
Sodium chloride (nacl), by Merck Group (1 mentions)
Toluene, by Merck Group (1 mentions)
Milli q integral 5 system, by Merck Group (1 mentions)
Formic acid, by Merck Group (1 mentions)
15n5 8 oxodg, by Cambridge Isotopes (1 mentions)
Oca20, by Dataphysics (1 mentions)
Dsa30, by Krüss (1 mentions)
S 4800 fesem, by Hitachi (1 mentions)
Tween 20, by Thermo Fisher Scientific (1 mentions)
Nanodrop spectrophotometer, by Thermo Fisher Scientific (1 mentions)
Potassium chloride (kcl), by Thermo Fisher Scientific (1 mentions)
2 propanol, by Merck Group (1 mentions)
Potassium iodide, by Thermo Fisher Scientific (1 mentions)
Chlorobenzene, by Merck Group (1 mentions)
Baker analyzed lc ms reagent, by Avantor (1 mentions)
Lead 2 iodide, by Thermo Fisher Scientific (1 mentions)
R 215, by Büchi (1 mentions)
7 protocols using ultrapure water
1
Oxidative DNA Damage Quantification Protocol
2
Hydrophobicity Characterization via Contact Angle
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Static contact angle was used to evaluate the hydrophobicity/hydrophilicity of the surface, with the sessile drop method (OCA 20, DataPhysics Instruments GmbH, Filderstadt, Germany) according to the Young–Laplace fitting at room temperature using ultrapure water (VWR, Oslo, Norway) as wetting agent (n = 3).
3
Surface Characterization of Fluoride-Modified Titanium
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The structure of the control and fluoride-modified titanium disks and implant surface was characterized using a field emission scanning electron microscope (Hitachi S-4800 FE-SEM; Hitachi Ltd., Tokyo, Japan). The surface roughness of titanium samples was measured using an atomic force microscope (Nanoscope MultiMode & Explore SPM; Vecco Instrument, Plainview, NY, USA). Measurements by atomic force microscopy (AFM) were conducted in ambient air under tapping mode at a scan rate of 0.8413 Hz and scan size of 0.25×0.25 μm. Different areas of the surface for the control group and fluoride-modified groups were measured for statistical analysis.
The static contact angle was calculated using the sessile drop method with a video-based contact angle system (DSA30; Krüss, Hamburg, Germany) according to Young–Laplace fitting at room temperature. Contact angle measurements were performed for four different titanium disk surfaces from each group using ultrapure water (VWR, Radnor, PA, USA) as a wetting agent. The chemical composition of these titanium sample surfaces was analyzed by energy-dispersive spectroscopy (EDS) with scanning electron microscopy (SEM).
The static contact angle was calculated using the sessile drop method with a video-based contact angle system (DSA30; Krüss, Hamburg, Germany) according to Young–Laplace fitting at room temperature. Contact angle measurements were performed for four different titanium disk surfaces from each group using ultrapure water (VWR, Radnor, PA, USA) as a wetting agent. The chemical composition of these titanium sample surfaces was analyzed by energy-dispersive spectroscopy (EDS) with scanning electron microscopy (SEM).
4
E. coli DNA Extraction and Purification
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Each E. coli isolate was submitted to an overnight incubation at 37 °C in Brain Heart Infusion Agar. Then, approximately 10 µL of the bacterial cultures formed by the isolates under testing, and by both E. coli J96 and E. coli KS52 reference strains, were collected using a loop, and resuspended in 100 μL of tris-ethylenediaminetetraacetic acid buffer (VWR, Philadelphia, PA, USA) supplemented with 0.1% Tween 20 (Fisher Bioreagents, Pittsburgh, MA, USA). As described by Dashti’s team [33 ], bacterial suspensions were then incubated at 100 °C for 10 min, after which they were centrifuged at 14,000 rpm for another 10 min. The resulting supernatant was collected, and the DNA concentration and purity were assessed using a NanoDrop™ Spectrophotometer (ThermoFisher Scientific, Waltham, MA, USA). DNA purity was determined based on the 280/260 absorbance ratio, which should be of approximately 1.8 for the DNA suspension to be considered pure. Subsequently, all DNA suspensions were diluted in sterile and ultrapure water (VWR, Philadelphia, PA, USA) until they reached a standardized concentration of 50 ng/μL and were subsequently kept at −20 °C until further use.
5
RNA Extraction from TRIzol Reagent
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The aqueous layer from the TRIzol reagent was supplemented with isopropanol, vortexed, incubated, and then centrifuged. The resultant pellet was washed with 75% ethanol and centrifuged. The mRNA pellet was resuspended with 20 μL RNA of ultrapure water (VWR) and stored at −80°C until use.
6
Perovskite Solar Cell Fabrication
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Lead (II) iodide (PbI2, 99.9985%), potassium iodide (KI, 99.995%), and potassium chloride (KCl, 99.997%) were purchased from Alfa Aesar. Anhydrous N,N-dimethylformide (DMF, 99.8%), 2-propanol (IPA, 99.5%), and chlorobenzene (CB, 99.8%) were purchased from Sigma-Aldrich (Saint Louis, MO, USA). Potassium bromide (KBr, IR spectroscopic) was purchased from Honeywell (Morristown, NJ, USA). Ultra-pure wa ter (Resistivity > 18.2 MΩ·cm at 25 °C, Baker Analyzed LC/MS Reagent) was purchased from J.T. Baker (Radnor, PA, USA). Poly(3,4-ethylenedioxythiophene) polystyrene sulfonate (PEDOT:PSS), methylammonium iodide (MAI, >98%), bathocuproine (BCP, >99.5%), and phenyl-C61-butyric acid methyl ester (PC61BM, >99.5%) were purchased from Uni-onward (New Taipei City, Taiwan). All materials were used as received.
7
Optimized Extraction and LC-MS Analysis
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Ultra-pure water (Elga, Veolia water, Paris, France) and LCMS grade acetonitrile/methanol (J.T. Baker, Center Valley, PA, USA) were used. LR. grade ethanol was purchased from Chanshu Hongsheng Fine Chemicals Co. Ltd., China. Extracts were concentrated using a vacuum rotary evaporator (Büchi R-215, Switzerland). LC-MS analysis was carried out using HPLC SHIMADZU LC-20AD which is coupled with an API-2000 mass spectrometer-MS/MS, [Applied Biosystem/MDS SCIEX, Canada]. Cell culture media and biochemical reagents were obtained from Himedia, Mumbai, India.
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