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2 protocols using hplc graded acetonitrile

1

Comprehensive Metabolite Profiling Protocol

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Uric acid, L-tryptophan, L-alanine, L-valine, L-leucine, L-isoleucine, glycine, l-serine, succinic acid, fumaric acid, malic acid, 2-ketoglutaric acid, L-glutamic acid, D-ribose, sodium 2-hydroxybutyrate, 3-hydroxybutyric acid, 2-isopropylmalic acid, formic acid, serotonin, γ-aminobutyric acid-2,2,3,3,4,4d6, 21-deoxycortisol, 11-deoxycortisol, oleic acid, cis-4,7,10,13,16,19-docosahexaenoic acid, cis-10-nonadecenoic acid (C19:1n9c) and sodium taurochenodeoxycholate were purchased from Sigma-Aldrich (St. Louis, MO, USA). Taurocholic acid was obtained from Steraloids (Newport, RI, USA). p-Cresyl sulfate potassium salt and arachidonic acid were supplied from Toronto Research Chemicals (North York, ON, Canada). Urea, HPLC-graded acetonitrile, methanol and isopropanol were obtained from Fisher Scientific (Hampton, NH, USA). Water was purified in-house using a Milli-Q Advantage A10 water purification system (Millipore, Bedford, MA, USA). Palmitic acid, cis-9,12-linoleic acid, 2-aminobutyric acid, pyridine (HPLC grade) with AcroSeal and N-methyl-N-(trimethylsilyl) trifluoroacetamide (MSTFA) were acquired from Acros Organics (Morris Plains, NJ, USA).
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2

Analytical Standards Procurement and Characterization

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Ephedrine hydrochloride was purchased by National Institute for the Control of Pharmaceutical and Biological products of China (Beijing, China). Sinapine thiocyanate, tetrahydropalmatine, baicalin and asarinin were purchased from Chengdu Chroma-Biotech Co. Ltd. (Chengdu, China). All compounds except Ephedrine hydrochloride had a purity of 98%; the purity of Ephedrine hydrochloride was 99%.
Glycocholic acid was purchased from Steraloids (Newport, RI, USA) while norepinephrine, epinephrine, alanine serine and valine were obtained from Sigma-Aldrich (St. Louis, MO, USA). HPLC-graded acetonitrile and methanol were obtained from Fisher Scientific (Hampton, NH, USA). Cholic acid D4 and formic acid were purchased from Cambridge Isotope Laboratory Inc. (Cambridge, UK) and Sigma-Aldrich (St. Louis, MO, USA), respectively. Water was purified in-house using a Milli-Q Advantage A10 water purification system (Millipore, Bedford, MA, USA).
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