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Tritc conjugated antibodies

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TRITC conjugated antibodies are fluorescently labeled secondary antibodies. TRITC (Tetramethylrhodamine isothiocyanate) is the fluorescent dye used for conjugation, which emits in the red/orange region of the visible light spectrum. These antibodies can be used to detect and visualize target proteins in various immunoassay and microscopy applications.

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Lab products found in correlation

Axiovert 200m, by Zeiss (2 mentions) P erk, by Cell Signaling Technology (2 mentions) 4 6 diamidino 2 phenylindole (dapi), by Merck Group (2 mentions) Deptor, by Novus Biologicals (2 mentions)

Spelling variants of this product

FITC/TRITC-conjugated secondary antibodies (4 citations) Tetramethylrhodamine isothiocyanate (TRITC) conjugated secondary antibodies (2 citations) FITC- and TRITC-conjugated secondary antibodies (2 citations)

2 protocols using tritc conjugated antibodies

1

Immunofluorescence and Immunohistochemistry Protocol

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Cultured cells were fixed, permeabilized with 0.2% Triton-X 100 and blocked with 10% normal serum for 1h at room temperature (19 (link)). Primary antibodies used were S100β (Dako), MBP (Chemicon), and BLBP (Millipore). Secondary incubations used host-appropriate TRITC conjugated antibodies (Jackson Immunoresearch). Nuclei were labeled with DAPI (Sigma-Aldrich) and microscopic images acquired with ImageJ software (NIH) on a Zeiss Axiovert 200M. Tumor paraffin sections stained for MAF (Imgenex), pS6, pERK (Cell Signaling), and DEPTOR (Novus) were visualized with DAB.
Brundage M.E., Tandon P., Eaves D.W., Williams J.P., Miller S.J., Hennigan R.H., Jegga A., Cripe T.P, & Ratner N. (2014). MAF mediates crosstalk between Ras-MAPK and mTOR signaling in NF1. Oncogene, 33(49), 5626-5636.
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2

Immunofluorescence and Immunohistochemistry Protocol

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Cultured cells were fixed, permeabilized with 0.2% Triton-X 100 and blocked with 10% normal serum for 1h at room temperature (19 (link)). Primary antibodies used were S100β (Dako), MBP (Chemicon), and BLBP (Millipore). Secondary incubations used host-appropriate TRITC conjugated antibodies (Jackson Immunoresearch). Nuclei were labeled with DAPI (Sigma-Aldrich) and microscopic images acquired with ImageJ software (NIH) on a Zeiss Axiovert 200M. Tumor paraffin sections stained for MAF (Imgenex), pS6, pERK (Cell Signaling), and DEPTOR (Novus) were visualized with DAB.
Brundage M.E., Tandon P., Eaves D.W., Williams J.P., Miller S.J., Hennigan R.H., Jegga A., Cripe T.P, & Ratner N. (2014). MAF mediates crosstalk between Ras-MAPK and mTOR signaling in NF1. Oncogene, 33(49), 5626-5636.
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