Lithium heparin

Manufactured by Thermo Fisher Scientific

Sourced in United States

Lithium heparin is an anticoagulant agent used as a blood collection tube additive. It functions by inhibiting the coagulation cascade, preventing blood from clotting during sample collection and processing.

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Lab products found in correlation

Methanol, by Cambridge Isotopes (1 mentions) Inosine 15n4, by Cambridge Isotopes (1 mentions) Thymine d4, by Cambridge Isotopes (1 mentions) Glycocholate d4, by Cambridge Isotopes (1 mentions)

2 protocols using lithium heparin

Heparin-Stabilized Blood Collection

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Whole blood was collected in BD Vacutainer tubes with lithium heparin (Fisher Scientific, Pittsburgh, PA, USA). Liver function tests were conducted by the VA Portland Health Care System Phlebotomy Lab per standard operating procedures.

Wilhelm C.J., Fuller B.E., Huckans M, & Loftis J.M. (2017). Peripheral immune factors are elevated in women with current or recent alcohol dependence and associated with altered mood and memory. Drug and alcohol dependence, 176, 71-78.

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Plasma Metabolite Extraction Protocol

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Mice were culled immediately by cervical dislocation. Blood was taken via cardiac puncture, collected into a microtainer tube lined with lithium heparin (Fisher Scientific), and centrifuged (10 min, maximum speed, 4°C). Plasma was subsequently snap frozen. Metabolites were extracted from plasma by adding extraction buffer, consisting of 80% methanol containing inosine-15N4, thymine-d4, and glycocholate-d4 internal standards (Cambridge Isotope Laboratories) in a 1:4 ratio of plasma to extraction buffer. Samples were then centrifuged twice (5 min, maximum speed, 4°C), and supernatants were collected.

Dyck L., Prendeville H., Raverdeau M., Wilk M.M., Loftus R.M., Douglas A., McCormack J., Moran B., Wilkinson M., Mills E.L., Doughty M., Fabre A., Heneghan H., LeRoux C., Hogan A., Chouchani E.T., O’Shea D., Brennan D, & Lynch L. (2022). Suppressive effects of the obese tumor microenvironment on CD8 T cell infiltration and effector function. The Journal of Experimental Medicine, 219(3), e20210042.

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