Pmel transgenic mice

Female C57BL/6, BALB/c mice(6-8 weeks of age) were purchased from the Chinese Academy of Medical Sciences (Beijing, China). OT-1 TCR-transgenic mice, Pmel transgenic mice, and CD45.1⁺ congenic mice were purchased from Jackson Laboratory. The mice were housed and maintained in laminar flow cabinets under specific, pathogen-free conditions. The mice were cared for and used in accordance with army medical university ethical guidelines. To establish tumor models, C57BL/6 mice were injected s.c. with MC38-OVA or B16 cells.
OVA-derived peptide(H-2Kb, SIINFEKL), control H-2Kb RAHYNIVTF peptides, and gp100 peptide were purchased from Sigma-Aldrich. All Abs used for flow cytometry were obtained from Biolegend. ROS inhibitor (apocynin) was purchased from MCE.

C57BL/6 mice, Pmel transgenic mice carrying TCR gene specific for the mouse homolog (Pmel-17) of human gp100 (28 (link)), parental FVB mice and FVBN202 transgenic mice were purchased from the Jackson Laboratory (Bar Harbor, ME). Melanoma cell line B16-gp100 (8 (link)), mammary tumor MMC line (4 ), and DC1.2 DC line were maintained in DMEM media supplemented with 10% heat-inactivated FBS (Life Technologies, Grand Island, NY), 2 mM L-glutamine, 100 U/ml penicillin, and 100 mg/ml streptomycin. All experimental procedures were conducted according to the protocols approved by the Virginia Commonwealth University Institutional Animal Care and Use Committee (Richmond, VA).