Cell samples and tissue sections were fixed in 4% paraformaldehyde. The samples were then sealed with a 2% (W/V) bovine serum albumin (BSA) solution before being incubated with the primary antibody at 4 °C overnight and the secondary antibody the next day at room temperature. DAPI nuclear dye was utilized to restain the samples. The following primary antibodies were utilized: anti-VEGF antibody, anti-TGF-β antibody, anti-p-AKT antibody, anti-BACH1 antibody (Proteintech, USA), anti-PDGF-B antibody, anti-GPX4 antibody (Abclonal, China).
Anti tgf β antibody
Manufactured by Proteintech
Sourced in United States
The Anti-TGF-β antibody is a laboratory reagent used to detect and quantify the presence of transforming growth factor beta (TGF-β) in various biological samples. TGF-β is a multifunctional cytokine involved in various cellular processes such as cell growth, differentiation, and immune regulation.
Automatically generated - may contain errors
2 protocols using anti tgf β antibody
1
Multimodal Immunofluorescence Analysis
2
Immunostaining of Wound Tissue Samples
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Tissue sections and cells samples were fixed with 4% paraformaldehyde. After being sealed with 2% (W/V) bovine serum albumin (BSA) solution, the wound samples were incubated with primary antibody at 4 °C overnight, followed by incubation with according the second antibody at room temperature. The samples were restained with DAPI nuclear dye. The details of primary antibodies were as follows: anti-5-hmc antibody (Abcam, UK), anti-VEGF antibody (Proteintech, USA), anti-TGF-β antibody (Proteintech, USA).
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