A lactic acid dehydrogenase (LDH) ELISA kit (Nanjing Jiancheng Bioengineering Institute, Nanjing, Jiangsu, China) was used to detect cell necrosis with reference to the manual.
Ldh elisa kit
Manufactured by Nanjing Jiancheng
Sourced in China
The LDH ELISA kit is a laboratory equipment used for the quantitative determination of lactate dehydrogenase (LDH) in various biological samples. It employs the enzyme-linked immunosorbent assay (ELISA) technique to measure the LDH levels.
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4 protocols using ldh elisa kit
1
Quantifying Cell Necrosis via LDH ELISA
2
HUVEC Proliferation and Apoptosis Assay
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HUVECs (Jennio, China), Dulbecco modified eagle medium (DMEM) with low sugar (Hyclone, USA), fetal serum bovine (FBS, BI, India), penicillin-streptomycin (Bi YT, China), 0.25% pancreatin (Bi YT, China), rhACE2 (R&D Systems, USA), Ang II (Torics, UK), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyletrazolium bromide (MTT) cell proliferation and cytotoxicity assay kit (Sigma, USA), propidium iodide/ribonuclease (PI/RNase) cycle detection kit (BD, USA), fluorescein isothiocyanate (FITC) annexin V apoptosis detection kit 1 (BD, USA), reactive oxygen species (ROS) detection kit (Bi YT, China), interleukin (IL)-8 enzyme-linked immunosorbent assay (ELISA) kit (R&D, USA), tumor necrosis factor α (TNF-α) ELISA kit (R&D, USA), transforming growth factor-β1 (TGF-β1) ELISA kit (Abcam, USA), lactate dehydrogenase (LDH) ELISA kit (Jiancheng, China). HF90/HF240 cell incubator (Heal Force, China), Multiskan GO 1510 spectrophotometer (Thermo Fisher, USA), BD FACS Aria III flow cytometer (BD, USA).
3
Cytokine and LDH Quantification by ELISA
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The IL-1β (#EK0394) ELISA kits were from Boster Ltd. (Wuhan, China). The murine C3 ELISA kit (#ab157711) was purchased from Abcam. The LDH ELISA kit (#A020-2) was from Nanjing Jiancheng Bioengineering Institute (Nanjing, Jiangsu province, China). The concentration of cytokines and LDH was measured by the ELISA kits according to the manufacturers’ instructions.
4
Biochemical Marker Quantification
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In accordance with the kit instructions, AST, ALT and LDH levels were examined using respective kits. AST ELISA kit (Cat. No. C0010-2-1) and ALT ELISA kit (Cat. No. C009-2-1) were obtained from Jiancheng Bioengineering Institute (Nanjing, China); LDH ELISA kit (Cat. No. C0016) was purchased from Beyotime Biotechnology (Beijing, China).
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