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Anti rorγt pe clone afkjs 9

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The Anti-RORgT-PE (clone: AFKJS-9) is a fluorochrome-conjugated monoclonal antibody that specifically binds to the transcription factor RORgT. RORgT is a key regulator of Th17 cell development and function. This antibody can be used for the identification and analysis of RORgT-expressing cells by flow cytometry.

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Lab products found in correlation

Foxp3 staining kit, by Thermo Fisher Scientific (2 mentions) Anti cd117 bv421 and pe cy7 104d2, by BioLegend (2 mentions) Cd127 pe cy5 r34.34 and nkg2a pe cy7 z199, by Beckman Coulter (2 mentions) Anti il1r1 pe fab269p, by R&D Systems (2 mentions) Anti eomes pe eflour 610 wd1928, by Thermo Fisher Scientific (2 mentions)

Spelling variants of this product

RORγt (28 citations) Anti-RORγt (26 citations) RORγt-PE (19 citations) AFKJS-9 (12 citations) RORγt (AFKJS-9 (11 citations) Anti-RORγt-PE (9 citations) Anti-RORγt (AFKJS-9) (5 citations)

2 protocols using anti rorγt pe clone afkjs 9

1

Multiparametric Immune Cell Profiling

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were extracellularly stained with the following FITC-conjugated lineage antibodies for sorting as previously described (69 (link)): anti-CD3 (UCHT1), anti-CD1a (HI149), anti-CD14 (HCD14), anti-CD19 (HIB19), anti-TCRαβ (IP26), anti-TCRγδ (B1), anti-CD123 (6H6), anti-CD303/BDCA-2 (201A), anti-FceR1a [AER-37(CRA-1)], anti-CD235a (HI264), and anti-CD66b (G10F5), all from BioLegend. These antibodies were further used within the study: anti-CD3-APC/Cy7 (UCHT1), anti-CD14-APC/Cy7 (HCD14), anti-CD19-APC/Cy7 (HIB19), anti-CD56-PE/Dazzle™ 594 or BV650™ (HCD56), anti-CD34-BV510™/-PE or -FITC (581), anti-NKp44-APC (P44-8), anti-CD73-PE/Dazzle™ 594 (AD2), anti-CD39-APC (A1), anti-Thy1-Alexa Flour® 700 (5E10), and anti-CD117-BV421 and -PE/Cy7 (104D2), all from BioLegend. CD127-PE/Cy5 (R34.34) and NKG2A-PE/Cy7 (Z199) were from Beckman Coulter (California, USA). Anti-IL1R1-PE (FAB269P) was purchased from R&D (Minneapolis, Minnesota). Intranuclear staining of anti-Eomes-PE-eFlour® 610 (WD1928, Invitrogen) and anti- RorγT-PE (clone: AFKJS-9, eBioscience) was performed with the Foxp3 staining kit (Thermo Fischer Scientific) and protocol. The initial gating strategy is shown in Supplementary Figure 1.
Bennstein S.B., Weinhold S., Degistirici Ö., Oostendorp R.A., Raba K., Kögler G., Meisel R., Walter L, & Uhrberg M. (2021). Efficient In Vitro Generation of IL-22-Secreting ILC3 From CD34+ Hematopoietic Progenitors in a Human Mesenchymal Stem Cell Niche. Frontiers in Immunology, 12, 797432.
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2

Multiparametric Immune Cell Profiling

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were extracellularly stained with the following FITC-conjugated lineage antibodies for sorting as previously described (69 (link)): anti-CD3 (UCHT1), anti-CD1a (HI149), anti-CD14 (HCD14), anti-CD19 (HIB19), anti-TCRαβ (IP26), anti-TCRγδ (B1), anti-CD123 (6H6), anti-CD303/BDCA-2 (201A), anti-FceR1a [AER-37(CRA-1)], anti-CD235a (HI264), and anti-CD66b (G10F5), all from BioLegend. These antibodies were further used within the study: anti-CD3-APC/Cy7 (UCHT1), anti-CD14-APC/Cy7 (HCD14), anti-CD19-APC/Cy7 (HIB19), anti-CD56-PE/Dazzle™ 594 or BV650™ (HCD56), anti-CD34-BV510™/-PE or -FITC (581), anti-NKp44-APC (P44-8), anti-CD73-PE/Dazzle™ 594 (AD2), anti-CD39-APC (A1), anti-Thy1-Alexa Flour® 700 (5E10), and anti-CD117-BV421 and -PE/Cy7 (104D2), all from BioLegend. CD127-PE/Cy5 (R34.34) and NKG2A-PE/Cy7 (Z199) were from Beckman Coulter (California, USA). Anti-IL1R1-PE (FAB269P) was purchased from R&D (Minneapolis, Minnesota). Intranuclear staining of anti-Eomes-PE-eFlour® 610 (WD1928, Invitrogen) and anti- RorγT-PE (clone: AFKJS-9, eBioscience) was performed with the Foxp3 staining kit (Thermo Fischer Scientific) and protocol. The initial gating strategy is shown in Supplementary Figure 1.
Bennstein S.B., Weinhold S., Degistirici Ö., Oostendorp R.A., Raba K., Kögler G., Meisel R., Walter L, & Uhrberg M. (2021). Efficient In Vitro Generation of IL-22-Secreting ILC3 From CD34+ Hematopoietic Progenitors in a Human Mesenchymal Stem Cell Niche. Frontiers in Immunology, 12, 797432.
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