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Anti ddx4 cvh ab13840

Manufactured by Abcam
Sourced in United Kingdom, United States

Anti-DDX4/Cvh (ab13840) is a primary antibody that recognizes the DDX4/Cvh protein. DDX4/Cvh is a member of the DEAD box protein family and is involved in spermatogenesis and oogenesis.

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2 protocols using anti ddx4 cvh ab13840

1

Comprehensive Pluripotency Marker Analysis

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Commercially available antibodies were used: anti-DDX4/Cvh (ab13840, Abcam, Cambridge, UK); Goat Anti-Mouse IgG (Cy3 labeled, Bio-Synthesis, Inc., Louisville, KY, USA; dilution ratio:1:100); anti-NANOG and anti-OCT4 (Abcam, dilution ratio 1:100); anti-SSEA (BioLegend, San Diego, CA, USA, dilution ratio 1:100); anti-integrin α6 and anti-integrin β1 (BioLegend, dilution ratio 1:100).
Dulbecco’s modified Eagle medium (DMEM, 41965062) and fetal bovine serum (FBS, 10100-147) from Gibco (Grand Island, NY, USA); FuGENE® HD (E2311) and Dual-Luciferase® Reporter Assay System from Promega (Madison, WI, USA); Leukemia Inhibit Factor from mouse (mLIF, L5158), Fibroblast Growth Factor-Basic human (bFGF, F0291), Stem Cell Human (SCF, S7901); TSA from Sigma (St. Louis, MO, USA); PARIS™ from Ambion (Austin, TX, USA); retinoic acid (R2625) from Sigma.
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2

Pluripotency Induction and Maintenance

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Commercially available antibodies were used: anti-C-Kit (Southern Biotech), anti-DDX4/CVH (ab13840, Abcam); GAPDH Anti-Phosphoserine (SPM101, Abcam); Goat Anti-Mouse IgG (Cy3 labeled, Bio-Synthesis, Inc., Texas, USA;dilution ratio:1:100).
Recombinant Human Bone Morphogenetic Protein-4 (BMP4, cyt-361) is from PROSPEC Dulbecco's modi ed Eagle medium (DMEM. 41965062) and Fetal bovine serum (FBS. 10100-147) from Gibco; FuGENE® HD (E2311) and Dual-Luciferase® Reporter Assay System from Promega; Leukemia Inhibit Factor from mouse (mLIF L5158), Fibroblast Growth Factor-Basic human (bFGF, F0291), Stem Cell Human (SCF. S7901); 5-aza-2'-deoxycytidine and TSA from Sigma; Pierce RNA 3' end desthiobiotinylation kit from Thermo; RIP-Assay Kit (RN1001) from MBL; PARIS™ from Ambion; PAS / Glycogen Stain kit (D004-1-2) from Nanjing Jiancheng Bioengineering Institute.
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