Ab124968

Manufactured by Abcam

Sourced in United Kingdom

Ab124968 is a laboratory product manufactured by Abcam. It is a scientific instrument designed for use in research and analysis. The core function of this product is to perform a specific task or measurement, but a detailed description cannot be provided while maintaining an unbiased and factual approach.

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Lab products found in correlation

4 protocols using ab124968

Western Blot Analysis of Cell Signaling

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Western blot analyses were performed using an anti-alpha V antibody (ab124968, and ab112487 Abcam, Cambridge, MA), phospho-FAK antibody (ab24781, Abcam, Cambridge, MA) FAK antibody (ab131435, Abcam, Cambridge, MA), TLR2 antibody (ab213676, Abcam, Cambridge, MA), TLR4 antibody (SC-293072, Santa Cruz Biotecnology, Santa Cruz, CA), or MyD88 antibody (ab2064, Abcam, Cambridge, MA) followed by a horseradish peroxidase-conjugated anti-mouse antibody (SC-2005, Santa Cruz Biotechnology). Blots were then developed with the ECL-plus detection system (Thermo Fisher Scientific, Waltham, MA). To evaluate the samples for equal protein loading, membranes were stripped and re-probed with an anti-β-actin antibody (SC-1615, Santa Cruz Biotechnology).

Kamarajan P., Ateia I., Shin J.M., Fenno J.C., Le C., Zhan L., Chang A., Darveau R, & Kapila Y.L. (2020). Periodontal pathogens promote cancer aggressivity via TLR/MyD88 triggered activation of Integrin/FAK signaling that is therapeutically reversible by a probiotic bacteriocin. PLoS Pathogens, 16(10), e1008881.

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Integrin subunits and cell proliferation

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To evaluate the effect of integrin subunits on cell proliferation, cells were incubated with function blocking antibodies against integrin (concentration as recommended by supplier) for 30 min at 37 °C followed by plated onto LN-521-coated plates and subjected to the cell proliferation assay as described above. The antibodies to α2 (559987, BD Biosciences), α3 (sc-7019, Santa Cruz Biotechnology), α4 (553348, BD Biosciences), α6 (sc-59920, Santa Cruz Biotechnology), αv (ab124968, Abcam), and β5 (AB1926, Merck Millipore) integrin subunits were dialyzed using the Zeba™ Spin Desalting Columns (89889, Thermo Fisher Scientific) before use.

Ma M., Hua S., Min X., Wang L., Li J., Wu P., Liang H., Zhang B., Chen X, & Xiang S. (2022). p53 positively regulates the proliferation of hepatic progenitor cells promoted by laminin-521. Signal Transduction and Targeted Therapy, 7, 290.

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Protein Expression Analysis of Integrin Subunits and α-SMA

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The frozen tissue specimen was lysed in radio-immunoprecipitation assay lysis buffer. Lysates were centrifuged at 12,000 rpm for 10 minutes and protein concentration was determined using an enhanced bicinchoninic acid protein assay kit (Pierce Biotechnology, Inc., Rockford, IL, USA). Fifty microgram of proteins were separated using 8% SDS-PAGE and transferred to the polyvinylidene fluoride membrane. After that, the polyvinylidene fluoride membrane was blocked with 5% nonfat milk blocking buffer, and then incubated overnight at 4°C with rabbit antirat integrin β₃ (1:1,000; ab75872; Abcam), rabbit antirat integrin α_v (1:1,000; ab124968; Abcam), and mouse antirat α-SMA antibody (1:100; ab7817; Abcam). After washing, the membrane was incubated with horseradish peroxidase-conjugated secondary antibodies and detected by enhanced chemiluminescence assay. β-Actin was used as the loading control, and the bands were detected using enhanced chemiluminescence (EMD Millipore, Billerica, MA, USA).

Zhang C., Liu H., Cui Y., Li X., Zhang Z., Zhang Y, & Wang D. (2016). Molecular magnetic resonance imaging of activated hepatic stellate cells with ultrasmall superparamagnetic iron oxide targeting integrin αvβ3 for staging liver fibrosis in rat model. International Journal of Nanomedicine, 11, 1097-1108.

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Immunoblotting of Cell Signaling Proteins

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Immunoblotting was performed as previously described [21 (link)]. In brief, whole protein lysates were separated by SDS/PAGE and transferred to nitrocellulose membranes (Whatman, Dassel, Germany). Membranes were incubated with the following primary antibodies diluted in 5% Milk/TBST-containing blocking solution: mouse monoclonal anti-actin (1:10000, 691391, MP Biomedicals, Illkirch, France), anti-CAS mouse monoclonal antibody (1:500, ab54674, Abcam, Cambridge, UK), rabbit monoclonal antibodies of anti-integrin Δ1 (1:2 000, ab179471, Abcam), anti-integrin αV (1:2 000, ab124968, Abcam), and anti-HO-1 (1:20 000, ab68477, Abcam). Detection was performed using Odysee Sa Infrared Imaging System (LI-COR Bioscience, Bad Homburg, Germany).

Winkler J., Roessler S., Sticht C., DiGuilio A.L., Drucker E., Holzer K., Eiteneuer E., Herpel E., Breuhahn K., Gretz N., Schirmacher P., Ori A, & Singer S. (2016). Cellular apoptosis susceptibility (CAS) is linked to integrin β1 and required for tumor cell migration and invasion in hepatocellular carcinoma (HCC). Oncotarget, 7(16), 22883-22892.

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