Mem sodium pyruvate

Manufactured by Merck Group

MEM Sodium pyruvate is a laboratory reagent used as a culture medium supplement in cell culture applications. It provides sodium pyruvate, an important energy source for cells, to support their growth and metabolism. The product is formulated to maintain the pH and osmolality of the cell culture medium.

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Lab products found in correlation

Lymphoprep, by BD (2 mentions) Interleukin 2and 15, by Miltenyi Biotec (2 mentions) Pen strep, by Merck Group (2 mentions) Interleukin 2 (il 2), by Thermo Fisher Scientific (2 mentions) Non essential amino acids (neaa), by Merck Group (2 mentions) L glutamine, by Merck Group (2 mentions) Live dead fixable aqua dead cell stain kit, by Thermo Fisher Scientific (2 mentions) Falcon tube, by BD (1 mentions) Millipore filter, by Merck Group (1 mentions)

Spelling variants of this product

Sodium pyruvate (1753 citations) Pyruvate (358 citations)

2 protocols using mem sodium pyruvate

Isolation and Culture of T Cells

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RPMI-1640 medium, LymphoprepTM, 0.9% saline (NaCl), Falcon tube (50 ml), Millipore filter (0.22 µM), syringe, 10% Foetus calf serum (FCS), L-glutamine (200mM; 100X), Pen/strep
(10,000 units/ml pen and 10,000 units/ml strep), MEM Sodium pyruvate (100mM), non-essential amino acids (10mM; 100X). IL-2 (100 IU/ml) were from GIBCO Life Technologies (USA). Interleukin-2
and 15 were purchased from Miltenyi Biotec (Germany). Monoclonal antibodies (mAbs) specific for CD3, CD4, CD8, TCR-Vγ9 were from Beckman Coulter (USA). Live/Dead fixable aqua dead
cell stain kit was from Invitrogen-Life Technologies (USA). Immunoglobulin from human and zoledronic acid injection (4 mg) were purchased from Sigma Aldrich and Cipla respectively.

Khan M.W., Otaibi A.A., Sherwani S., Alshammari E.M., Al-Zahrani S.A., Khan W.A., Alsukaibi A.K., Alouffi S, & Khan S.N. (2021). Optimization of methods for peripheral blood mononuclear cells isolation and expansion of human gamma delta T cells. Bioinformation, 17(3), 460-469.

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Isolation and Expansion of γδ T Cells

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Chemicals and reagents: RPMI-1640 medium, Lymphoprep TM , 0.9% saline (NaCl), Falcon tube (50 ml), Millipore filter (0.22 µM), syringe, 10% Foetus calf serum (FCS), L-glutamine (200mM; 100X), Pen/strep (10,000 units/ml pen & 10,000 units/ml strep), MEM Sodium pyruvate (100mM), non-essential amino acids (10mM; 100X). IL-2 (100 IU/ml) were from GIBCO Life Technologies (USA). Interleukin-2 and 15 were purchased from Miltenyi Biotec (Germany). Monoclonal antibodies (mAbs) specific for CD3, CD4, CD8, TCR-Vγ9 were from Beckman Coulter (USA). Live/Dead fixable aqua dead cell stain kit was from Invitrogen-Life Technologies (USA). Immunoglobulin from human and zoledronic acid injection (4 mg) were purchased from Sigma Aldrich and Cipla respectively.

Khan M.W.A. (2021). Optimization of methods for peripheral blood mononuclear cells isolation and expansion of human gamma delta T cells. Bioinformation, 17(3), 460-470.

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