Agilent 6120 quadrupole msd

HPLC-MS analysis of the most active actinobacterial extracts was performed on the Agilent 6120 Quadrupole MSD mass spectrometer (Agilent Technologies, Santa Clara, CA, USA) equipped with an Agilent 1200 Series Quaternary LC system and an Eclipse XDB-C18 column (150 × 4.6 mm, 5 μm; solvent A: H₂O/0.1% formic acid, solvent B: CH₃CN/0.1% formic acid; flow rate: 0.4 ml min^− 1; 0–4 min, 10% B; 4–22 min, 10–100% B; 22–27 min, 100% B; 27–29 min, 100–10% B; 29–35 min, 10% B). The molecular masses on each of the ion peak were calculated in all the extracts and the data was analysed by comparison with the already reported Streptomyces compounds in the AntiBase [28 ].

Optical rotations were measured on DIP-1000 digital polarimeter (JASCO Corporation, Tokyo, Japan). UV data were determined with a JASCO UV/VIS-Spectrophotometer (JASCO Corporation, Tokyo, Japan). ¹H and ¹³C NMR data were obtained from Bruker AVANCE 250 spectrometer (Bruker BioSpin Corporation, Billerica, MA, USA) and Jeol ECA-500 spectrometer (Jeol Ltd., Akishima, Tokyo, Japan) using TMS as internal standard. LR-ESI-MS were recorded on Agilent 6120 quadrupole MSD consisting of 1260 Infinity pump, 1260 Infinity autosampler, 1260 Infinity DAD detector and an Openlab ChemStation for data acquisition and processing. HR-FAB-MS spectra were performed on JEOL JMS-600W high-resolution mass spectrometer. Preparative HPLC was achieved with Gilson TRILUTION LC system with 321 pump, and UV/Vis–155 detector using Phenomenex Luna (Phenomenex, Torrance, CA, USA) C18 column (250 × 21 mm, 20 μm).