Cells were carefully washed twice with DPBS and fixed with 4% paraformaldehyde at room temperature for 30 min to 1 h. After washing with DPBS twice, the cells were stained with 60% saturated Oil Red-O reagent (G1260, Solarbio, Beijing, China) for 15 min and then washed with 60% isopropanol and DPBS. The Oil Red-O absorbed by cells was extracted with 100% isopropanol and detected by a SpectraMax M5 (Molecular Devices, San Jose, CA, USA) at 510 nm.
Oil red o reagent
Manufactured by Solarbio
Sourced in China
Oil Red O reagent is a lipid-soluble azo dye commonly used in histology and cytochemistry for the selective staining of neutral lipids and triglycerides. It is used to visualize and identify the presence of lipid droplets in cells and tissues.
Automatically generated - may contain errors
Lab products found in correlation
3 protocols using oil red o reagent
1
Quantitative Lipid Staining Assay
2
Histological Analysis of Adipose, Colon, and Liver
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Adipose, colon, and liver tissues were rinsed in PBS and fixed with 4% paraformaldehyde (PFA) for 2 days, followed by embedding in paraffin. The tissues were then cut into 5-µm thickness slices. Colon mucosa integrity was measured using the Alcian blue reagent (Beyotime, China). The morphology of the adipocytes and liver damage were tested using hematoxylin and eosin (HE). All procedures were performed according to the manufacturer’s instructions. Lipid accumulation in adipocytes was measured using Oil Red O staining. Tissues were deparaffinized, fixed with 10% formalin, and washed with 60% isopropanol. The tissues and cells were then incubated with Oil Red O reagent (SolarBio) for 60 min and washed with distilled water. Images were obtained using a microscope (Carl Zeiss).
3
Oil Red O Staining of Lipid Droplets
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Cells were carefully washed twice with DPBS and fixed with 4% paraformaldehyde at room temperature for 30 min to 1 h. After washing with DPBS twice, the cells were stained with a 60% saturated Oil Red O reagent (G1260, Solarbio, Beijing, China) for 15 min and then washed with 60% isopropanol and DPBS. The Oil Red O absorbed by cells was extracted with 100% isopropanol and detected by a SpectraMax M5 (Molecular Devices, San Jose, CA, USA) at 510 nm.
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