Cyclophosphamide (CPD) was purchased from Shengdi Company (Jiangsu, China). M-CSF and RANKL were purchased from Peprotech (New Jersey, USA). Primary antibodies against active β-catenin, Runx2, Cyclin D1, c-Myc, IκBα, phospho-IκBα, JNK, phospho-JNK, p38 and phospho-p38 were purchased from Cell Signaling Technology (Beverly, MA, USA) and others against TRAF-6, TRAF-3, NFATc1, RANK and c-Fos were purchased from Santa Cruz Biotechnology (CA, USA). The antibodies against β-catenin, phospho-Gsk-3β, and DKK1 were purchased from Abcam Company (Cambridge, UK).
Antibodies against β catenin
Manufactured by Abcam
Sourced in United States
Abcam's antibodies against β-catenin are designed for use in various laboratory applications, such as Western blotting, immunohistochemistry, and immunofluorescence. β-catenin is a key component of the Wnt signaling pathway and plays a crucial role in cell-cell adhesion and gene transcription.
Automatically generated - may contain errors
Lab products found in correlation
C fos, by Santa Cruz Biotechnology (1 mentions)
Cyclin d1, by Cell Signaling Technology (1 mentions)
Phospho jnk, by Cell Signaling Technology (1 mentions)
Phospho p38, by Cell Signaling Technology (1 mentions)
Traf6, by Santa Cruz Biotechnology (1 mentions)
Traf3, by Santa Cruz Biotechnology (1 mentions)
Phospho gsk 3β, by Abcam (1 mentions)
C myc, by Cell Signaling Technology (1 mentions)
Phospho iκbα, by Cell Signaling Technology (1 mentions)
Nfatc1, by Santa Cruz Biotechnology (1 mentions)
M csf, by Thermo Fisher Scientific (1 mentions)
Rankl, by Thermo Fisher Scientific (1 mentions)
Runx2, by Cell Signaling Technology (1 mentions)
Procaspase 3, by Cell Signaling Technology (1 mentions)
Mmp 9, by Abcam (1 mentions)
P akt, by Cell Signaling Technology (1 mentions)
Gapdh, by Cell Signaling Technology (1 mentions)
Bcl 2, by Cell Signaling Technology (1 mentions)
Cleaved caspase 3, by Cell Signaling Technology (1 mentions)
Cisplatin, by Merck Group (1 mentions)
4 protocols using antibodies against β catenin
1
Molecular Mechanisms of Osteoclast Differentiation
2
Investigating Gastric Cancer Cell Lines and Novel Compounds
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Three human gastric cancer cells (HGC-27, BGC-823, and SGC-7901) were obtained from the China Center for Type Culture Collection. The human normal gastric epithelial GES-1 cell lines were purchased from iCell Bioscience Inc. (China, Shanghai). The cells were maintained in DMEM/F-12 medium containing 10% fetal bovine serum plus 1% antibiotics (100 IU penicillin and 100 µg/mL streptomycin) in a humidified incubator at 37°C with 5% CO2 atmosphere.
SIN (C19H23NO4·0.3 CHCl3) (Fig.1 a) from Pubchem Compound) was purchased from Sigma (Sigma-Aldrich) and dissolved in 100% dimethylsulfoxide (DMSO) to prepare a 100 mM stock solution for storage at −20°C. Cisplatin was obtained from Sigma and dissolved in normal saline, which was stored at −20°C at a concentration of 4 mg/mL.
Primary antibodies against AKT, p-AKT, Bax, Bcl-2, procaspase3, cleaved caspase3 and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) were purchased from Cell Signaling Technology. Antibodies against β-catenin, MMP-2, and MMP-9 were purchased from Abcam. The effective working concentration for the above was 1:1000.The secondary antibodies were from LI-COR from Selleckchem, diluted to 1:10 000.
SIN (C19H23NO4·0.3 CHCl3) (Fig.
Primary antibodies against AKT, p-AKT, Bax, Bcl-2, procaspase3, cleaved caspase3 and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) were purchased from Cell Signaling Technology. Antibodies against β-catenin, MMP-2, and MMP-9 were purchased from Abcam. The effective working concentration for the above was 1:1000.The secondary antibodies were from LI-COR from Selleckchem, diluted to 1:10 000.
3
Liver Cancer Chemoprevention Protocol
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LSW was purchased from shanghai ley's pharmaceutical Co., Ltd. (Shanghai, China). Diethylnitrosamine (DEN) was purchased from Tokyo Chemical Industry Co., Ltd. (Tokyo, Japan). Tween-80 was kindly provided by Amresco®. (Amresco, USA). Lecithin from egg yolk was purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). Sesame oil was kindly provided by Blessing Mill (Wuhan, China). Glycerol ReagentPlus (Gas chromatography [GC] grade) were obtained from Sigma-Aldrich Co. (St Louis, MO, USA). Ultrapure deionized water (Heal Force Bio-Meditech Holdings Ltd., Shanghai, China) was used for all the experiments. All other reagents were of analytical grade. Primary antibodies used in this study included antibodies against β-catenin, PCNA, HMGB-1, and COX-2 (Abcam Inc., Cambridge, MA, USA).
4
11-Methylenedioxy-Camptothecin Anti-Cancer Assay
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FL118, 11-methylenedioxy-camptothecin (Figure 1), was obtained from a cooperating lab, the American Roswell Park Cancer Institute (RPCI), and was dissolved with dimethyl sulphoxide (DMSO). Antibodies against survivin, E-cadherin, vimentin, and β-actin were purchased from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA). Antibodies against β-catenin, lamin B1, and cyclin D1 were purchased from Abcam, Inc. (Cambridge, MA, USA).
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