PVA (DS = 0.61 and number-average polymerization degree of 1799, 98~99%), hydroxyapatite (HA) > 12 nm particle size and α-d -lactose were analytical grade and received from Aladdin Chemistry Co. (Shanghai, China). Tris (hydroxymethyl) amino-methane (Tris) (99.9%) and HCl were analytical grade reagents purchased from Solarbio (Beijing, China) and used as supplied. Glutaraldehyde was obtained from Aladdin Chemistry Co. (Shanghai, China). Deionized water was used in all experiments.
Tris hydroxymethyl amino methane tris
Manufactured by Solarbio
Sourced in China
Tris (hydroxymethyl) amino-methane (Tris) is a common chemical compound used in various laboratory applications. It serves as a buffer solution to maintain a specific pH range in a variety of biological and chemical experiments.
Automatically generated - may contain errors
Lab products found in correlation
3 protocols using tris hydroxymethyl amino methane tris
1
Biomimetic Hydroxyapatite-Based Composites
2
Genistein and Amino Acid Derivatives Protocol
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Genistein (≥97%) and BSA (≥99%) were obtained from Aladdin's Reagent Co., Ltd. (Shanghai, China). Tris(hydroxymethyl) aminomethane (Tris) (≥99%) was obtained from Solarbio Science & Technology Co., Ltd. (Beijing, China). Compounds 4a–4d (≥97%) were synthesized in the laboratory in University of South China.
The stock solution of BSA (10 μM) was prepared using 0.05 mol L−1 Tris–HCl buffer solution containing 0.05 mol L−1 NaCl (pH = 7.4). The stock solutions of genistein and its amino acid derivatives 4a–4d (1000 μM) were dissolved in methanol. All reactants and solvents used in synthesis were analytically pure and the distilled water was used in experiments.
The stock solution of BSA (10 μM) was prepared using 0.05 mol L−1 Tris–HCl buffer solution containing 0.05 mol L−1 NaCl (pH = 7.4). The stock solutions of genistein and its amino acid derivatives 4a–4d (1000 μM) were dissolved in methanol. All reactants and solvents used in synthesis were analytically pure and the distilled water was used in experiments.
3
Functionalization of Dopamine Hydrochloride
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Dopamine hydrochloride and polyethyleneimine (Mw = 600 Da, 1800 Da, 10000 Da and 70000 Da) were purchased from Aladdin (China). Sodium hydroxide (NaOH), hydrochloric acid (HCl, 38%) and ethanol were obtained from Chuandong Chemical Industry Co., Ltd (China). Tris-(hydroxymethyl)aminomethane (Tris) and heparin (≥160 U mg−1) were purchased from Beijing Solarbio Science & Technology Co., Ltd (China). Polypropylene (PP, 0.22 μm) membrane and Polytetrafluoroethylene (PTFE, 0.22 μm) membrane were provided by Shanghai Xinya Purification Device Factory. A polyvinyl chloride (PVC) sheet was obtained from Shandong Jintiancheng Plastic Products Co., Ltd. 1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide (EDC), N-hydroxysuccinimide (NHS) and toluidine blue (TB) were purchased from Sigma-Aldrich Co., Ltd
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