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The M11217 is a high-precision laboratory instrument designed for accurate and reliable measurements. It features advanced sensor technology and a user-friendly interface, enabling efficient data collection and analysis. The core function of the M11217 is to provide precise measurements for various scientific applications.

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Lab products found in correlation

Vs120 slide scanner microscope, by Olympus (2 mentions) Alexa fluorophore secondary antibody, by Thermo Fisher Scientific (2 mentions) Rat anti mcherry, by Thermo Fisher Scientific (2 mentions) H 029 30, by Neuromics (2 mentions) Gt15023, by Thermo Fisher Scientific (2 mentions) Chicken anti gfp, by Thermo Fisher Scientific (2 mentions)

2 protocols using m11217

1

Immunofluorescence Labeling of Brain Sections

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Brain sections were washed in PBS with Tween-20, pH 7.4 (PBST) and blocked in 3% normal donkey serum/PBST for 1 h at room temperature. Then, brain sections were incubated overnight at room temperature in blocking solution containing primary antiserum (rat anti-mCherry, Life Technologies M11217, 1:1,000; rabbit anti-POMC precursor, Phoenix Pharmaceuticals H-029-30, 1:3,000, goat anti-AgRP, Neuromics GT15023, 1:3,000, chicken anti-GFP, Life Technologies A10262, 1:1,000, goat anti-Fos, Calbiochem PC38, 1:1,000). The next morning sections were extensively washed in PBS and then incubated in Alexa-fluorophore secondary antibody (Molecular Probes, 1:1,000) for 1 h at room temperature. After several washes in PBS, sections were mounted on gelatin-coated slides and fluorescence images were captured with Olympus VS120 slide scanner microscope.
Fenselau H., Campbell J.N., Verstegen A.M., Madara J.C., Xu J., Shah B.P., Resch J.M., Yang Z., Mandelblat-Cerf Y., Livneh Y, & Lowell B.B. (2016). A rapidly-acting glutamatergic ARC→PVH satiety circuit postsynaptically regulated by α-MSH. Nature neuroscience, 20(1), 42-51.
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2

Immunofluorescence Labeling of Brain Sections

Check if the same lab product or an alternative is used in the 5 most similar protocols
Brain sections were washed in PBS with Tween-20, pH 7.4 (PBST) and blocked in 3% normal donkey serum/PBST for 1 h at room temperature. Then, brain sections were incubated overnight at room temperature in blocking solution containing primary antiserum (rat anti-mCherry, Life Technologies M11217, 1:1,000; rabbit anti-POMC precursor, Phoenix Pharmaceuticals H-029-30, 1:3,000, goat anti-AgRP, Neuromics GT15023, 1:3,000, chicken anti-GFP, Life Technologies A10262, 1:1,000, goat anti-Fos, Calbiochem PC38, 1:1,000). The next morning sections were extensively washed in PBS and then incubated in Alexa-fluorophore secondary antibody (Molecular Probes, 1:1,000) for 1 h at room temperature. After several washes in PBS, sections were mounted on gelatin-coated slides and fluorescence images were captured with Olympus VS120 slide scanner microscope.
Fenselau H., Campbell J.N., Verstegen A.M., Madara J.C., Xu J., Shah B.P., Resch J.M., Yang Z., Mandelblat-Cerf Y., Livneh Y, & Lowell B.B. (2016). A rapidly-acting glutamatergic ARC→PVH satiety circuit postsynaptically regulated by α-MSH. Nature neuroscience, 20(1), 42-51.
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