All cell extracts were obtained by lysing cells in ice-cold lysis buffer. The undissolved matter was removed by high-speed centrifugation, and the protein percentage of the supernatant was measured by a BCA protein test kit. Each group of samples was loaded on a 10% SDS-PAGE gel, and the bands were transferred to a PVDF membrane. After being blocked with the sealing solution, the membranes were immunolabeled overnight with the following primary antibodies: HIF-1 (1:1000, Cell Signaling Technology, USA), p53 (1:1000, Cell Signaling Technology, USA), P-gp (1:1000, Cell Signaling Technology, USA), Bax (1:1000, Abcam, USA), F-Caspase3 (1:1000, Cell Signaling Technology, USA), C-Caspase3 (1:1000, Cell Signaling Technology, USA), F-Casase9 (1:1000, Cell Signaling Technology, USA), C-Caspase9 (1:1000, Cell Signaling Technology, USA), F-PARP (1:1000, Abcam, USA), C-PARP (1:1000, Abcam, USA), and β-actin antibodies (1:1000, Santa Cruz, USA). After washing, the protein bands on the PVDF membrane were incubated with suitable auxiliary antibodies (1:2000, ICN Pharmaceuticals), while an ECL kit was used for color development. The bands were analyzed after exposure to a ChemiDoc XRS+ gel imager (Bio-Rad, USA), and the protein content was expressed as the relative value of the corresponding internal reference band.
C caspase9
Manufactured by Cell Signaling Technology
Sourced in United States
C-Caspase9 is a lab equipment product that detects and measures the activity of caspase-9, a key enzyme involved in the apoptosis pathway. It provides a reliable and quantitative method for analyzing caspase-9 activation in various cellular and biochemical systems.
Automatically generated - may contain errors
Lab products found in correlation
C caspase 3, by Cell Signaling Technology (2 mentions)
Bcl2 associated x (bax), by Abcam (1 mentions)
β actin antibody, by Santa Cruz Biotechnology (1 mentions)
Chemidoc xrs gel imager, by Bio-Rad (1 mentions)
F caspase3, by Cell Signaling Technology (1 mentions)
C parp, by Abcam (1 mentions)
Hif 1, by Cell Signaling Technology (1 mentions)
P erk, by Cell Signaling Technology (1 mentions)
Proliferating cell nuclear antigen (pcna), by Santa Cruz Biotechnology (1 mentions)
P gsk3β, by Cell Signaling Technology (1 mentions)
P iκbα, by Cell Signaling Technology (1 mentions)
P pten, by Cell Signaling Technology (1 mentions)
Epcam, by Cell Signaling Technology (1 mentions)
Cytochrome c sc 13561, by Santa Cruz Biotechnology (1 mentions)
P pdk1, by Cell Signaling Technology (1 mentions)
Cd44 15675 1 ap, by Proteintech (1 mentions)
Mmp 9, by Cell Signaling Technology (1 mentions)
Gsk3β, by Cell Signaling Technology (1 mentions)
Vimentin, by Cell Signaling Technology (1 mentions)
β actin, by Cell Signaling Technology (1 mentions)
4 protocols using c caspase9
1
Protein Expression Analysis in Cellular Extracts
2
Antibody Characterization for EMT Study
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The antibodies against SPT6 (sc‐393938), staphylococcal nuclease and Tudor domain containing 1 (SND1; sc‐271590), cytochrome C (sc‐13561), and PCNA (sc‐56) were purchased from Santa Cruz (Dallas, TX, USA). The antibodies against vimentin (#5741), snail (#3879), slug (#9585), Oct4 (#2750), EpCAM (#2929), Caspase‐3 (#9662), c‐Caspase‐9 (#9505), GSK‐3β (#9323), p‐PTEN (#9554), p‐PDK1 (#3438), p85 (#4228), MMP‐9 (#3852), p‐GSK3β (#5558), IκBα (#4812), p‐IκBα (#2859), p‐Erk (#9102), and Erk (#9101) were purchased from Cell Signaling Technology (Beverly, MA, USA). The antibodies against β‐actin (20536‐1‐AP), PARP (13371‐1‐AP), E‐cadherin (20874‐1‐AP), N‐cadherin (22018‐1‐AP), CD133 (18470‐1‐AP), and CD44 (15675‐1‐AP) were purchased from Proteintech Group (Wuhan, Hubei, China). The antibody against TERT (ABE2075) was purchased from Millipore (Burlington, MA, USA).
3
Comprehensive Phytochemical Profile Analysis
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Reference substances including malic acid, citric acid, gallic acid, ephedrine, pseudoephedrine, neomangiferin, mangiferin, albiflorin, paeoniflorin, prim-O-glucosylcimifugin, liquiritin, 5-O-β-D-glucosyl-5-O-methylcisamminol, anemarsaponin E1, anemarsaponin BII, liquiritigenin, anemarsaponin C, licorice saponin G2, glycyrrhizic acid and anemarspaonin I were acquired from the Chengdu Desite Co. Ltd (Chengdu, China), and the purity of these compounds was ≥98%. Annexin V-FITC/PI assay kit was obtained from Multisciences (Lianke) Biotechnology Corporate Limited (Hangzhou, China). CCK8 detection kit and JC-1 probe was obtained from US Everbright®Inc (Suzhou, China). Enhanced chemiluminescence (ECL) luminescence reagent was purchased from Beijing 4A Biotech Co (Beijing, China). NF-κB activity assay kits were purchased from Beyotime Biotech (Haimen, China), and the human tumor necrosis factor (TNF)-α was obtained from Pepro Tech (Rocky Hill, NJ, China). BCA protein quantitative kit and primary antibodies for JAK2, STAT3, Bcl-2, Bax were bought from BOSTER biological technology company (Wuhan, China). C-caspase-9, C-caspase-3, p-JNK1/2, JNK1/2, β-actin, and the IgG-HRP were obtained from Cell Signaling technology (Chiyoda-ku, Tokyo, Japan). The QuickEasy™ Cell Direct RT-qPCR Kit-SYBR Green I kit was obtained from Chengdu Foregene Company Limited.
4
Sorafenib Cytotoxicity and Apoptosis Analysis
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Sorafenib was purchased from Selleck Chemicals (Houston, TX, USA). The BCA protein assay kit, the Cell Counting Kit-8, and the Annexin V-FITC apoptosis detection kit were purchased from KeyGen Biotech (Nanjing, China). The EdU Apollo567 In Vitro Imaging Kit was purchased from Guangzhou, People's Republic of China. DAPI and paraformaldehyde (PFA) were purchased from GuGe Biotech Co. Ltd. (Wuhan, China). Triton X-100 was purchased from ShenGong Biotech. Co. Ltd. (Shanghai, China). Methanol and ethanol were purchased from Shanghai LingFeng Chemical Reagent Co. Ltd. DMSO, Tween 20 and glycine was purchased from Sangon Biotech Co. Ltd. (Shanghai, China). Skim milk was purchased from Becton, Dickinson (San Diego, CA, USA). Primary antibodies against c-caspase-3, c-caspase-9, and c-PARP were purchased from Cell Signaling Technology (Danvers, MA, USA); GAPDH and SGOL1 were purchased from Abcam (Cambridge, MA, USA).
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