Total tbc1d1

Antibodies against phospho‐Akt Ser⁴⁷³ (#9271), phospho‐Akt Thr³⁰⁸ (#9275), phospho‐TBC1D1 Thr⁵⁹⁰ (#6927), phospho‐JNK Thr¹⁸³/Tyr¹⁸⁵ (#9251), phospho‐p38 MAPK Thr¹⁸⁰/Tyr¹⁸² (#9216), phospho‐ERK1/2 Thr²⁰²/Tyr²⁰⁴ (#9101), total Akt (#9272), total TBC1D1 (#4629S), total‐JNK (#9252), total‐p38 MAPK (#9212), total‐ERK1/2 (#9102), total IκBα (#9242), and total‐acetyl CoA carboxylase (ACC, #3662) were from Cell Signaling Technology (Beverly, MA). Antibodies against phospho‐AS160 Thr⁶⁴² (#07‐802), phospho‐ACC Ser⁷⁹ (#07‐303), phospho‐TBC1D1 Ser²³⁷ (#07‐2268), and total AS160 (#07‐741) were from Millipore (Temecula, CA). Anti‐phospho‐AS160 Ser⁵⁸⁸ (#3028P2) was from Symansis Limited (Timaru, New Zealand). Anti‐GLUT4 antibody (#4670‐1704) was from Bio‐Rad AbD Serotec (Oxford, UK). Anti‐SPT2 antibody (ab23696) was from Abcam (Cambridge, MA). Horseradish peroxidase (HRP)‐conjugated anti‐rabbit IgG was from Biosource International (Camarillo, CA). HRP‐conjugated anti‐sheep IgG was from Millipore. HRP‐conjugated anti‐mouse IgG was from Santa Cruz Biotechnology. Enhanced chemiluminescence reagents (ECL, ECL plus, and ECL Prime) were obtained from GE Healthcare Life Sciences (Uppsala, Sweden). All other reagents were obtained from Sigma‐Aldrich (St. Louis, MO).

The sheep antibody against phosphorylated Ser²³¹ on TBC1D1 was previously reported [11 (link)]. The flotillin-1 antibody (Cat No. sc-25506) was from Santa Cruz (Dallas, Texas, USA). The antibodies that recognise phosphorylated Ser⁴⁷³ on PKB (Cat No. 9271), total PKB (Cat No. 9272), and total TBC1D1 (Cat No. 4629), and the phospho-Akt substrate (PAS) antibody (Cat No. 9611) were from Cell Signaling Technology. The total AS160/TBC1D4 (Cat No. 07–741) was from Millipore. The antibody that recognises phosphorylated Thr⁶⁴⁹ on AS160/TBC1D4 (Cat No. 441071G) was from Life Technologies. The GAPDH antibody (Cat No. G8795) was from Sigma, and the cTnT antibody (Cat No. MA5-12960) was from Thermo Scientific.