Flow tube

Manufactured by Corning

Sourced in United States

The Flow Tube is a laboratory equipment designed to facilitate the controlled flow of gases or liquids. Its primary function is to provide a contained environment for the movement and observation of substances. The Flow Tube enables researchers to study the characteristics and behavior of materials under controlled flow conditions.

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Lab products found in correlation

Trypsin, by Thermo Fisher Scientific (1 mentions) Mouse anti human cd44, by BD (1 mentions) Mouse anti human cd34, by BD (1 mentions) Mouse anti human cd73, by BD (1 mentions) Vacutainer, by BD (1 mentions) Anti cd45 fitc, by BD (1 mentions) Anti cd106 pe, by BD (1 mentions) Anti cd11b v450, by BD (1 mentions) Anti cd90 pe cy7, by BD (1 mentions) Anti cd29 af647, by BD (1 mentions) Cytoflex, by Beckman Coulter (1 mentions)

Close spelling variants of this product

Sterile flow cytometry polypropylene round-bottom tubes Flow Cytometry tubes

5 protocols using flow tube

Isolation and Characterization of Urine-Derived Stem Cells

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Primary culture of the USCs was performed as previously described [33 (link),34 (link)]. Briefly, the USCs were obtained from four young male adult donors (20–30 years old). Fresh urine sample (about 200 mL) was centrifuged and washed with PBS for twice. The sediment was re-suspended and cultured in T25 cell culture flasks with 5 mL of established medium [35 (link)].
Cells in logarithmic growth phase were digested with trypsin (Gibco, USA), with their density adjusted to 1 × 10⁶ cells/mL with PBS. 100 μL aliquot of cell suspension was moved to each flow tube (Corning, USA). Thereafter, 2 μL of mouse anti-human CD29 (559883, BD Pharmingen™, USA), mouse anti-human CD34 (555821, BD Pharmingen™), mouse anti-human CD44 (555478, BD Pharmingen™), mouse anti-human CD73 (550257, BD Pharmingen™), mouse anti-human CD90 (561558, BD Pharmingen™) and mouse anti-human CD133(130-111-085, Miltenyi Biotec GmbH, Germany) antibodies were added to each tube. The mixture was allowed to incubate for 30 min in darkness at room temperature. Thereafter, the cells were washed twice with PBS and re-suspended in 200 μL PBS for flow analysis.

Song Y.T., Li Y.Q., Tian M.X., Hu J.G., Zhang X.R., Liu P.C., Zhang X.Z., Zhang Q.Y., Zhou L., Zhao L.M., Li-Ling J, & Xie H.Q. (2021). Application of antibody-conjugated small intestine submucosa to capture urine-derived stem cells for bladder repair in a rabbit model. Bioactive Materials, 14, 443-455.

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Platelet-rich Plasma Isolation Protocol

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We used a BD Vacutainer® (BD, New Jersey, USA) containing acid-citrate-dextrose solution formula A (ACD-A) anticoagulant to collect 54 ml of human blood from 6 volunteers (Supplementary Table 1) who were in good health and had provided informed content to participate in the study. The blood samples were first centrifuged (Eppendorf, Hamburg, Germany) at 450×g for 7 minutes, after which the plasma and the thin layer above the precipitated hemoglobin were transferred to a flow tube (Corning, New York, USA). This material was centrifuged for 5 minutes at 1600×g, and approximately 3/4 of the resulting mixture was collected as PPP (platelet-poor plasma). The remaining portion (PRP), containing a large amount of inactivated platelets, was activated with 0.5 M CaCl₂:1000 U of bovine thrombin = 1:1 (V/V) (mixed reagent) [22 (link)] at a ratio of 10:1 (V/V) for 10 minutes at room temperature before being centrifuged at 2015×g for 15 minutes. The supernatant was then collected and stored at −20°C for use. This study conformed to ethical guidelines and was approved by the Ethics Committee of Sun Yat-sen University.

Wang Y., Wang J., Li Y., Wang S, & Zhu X. (2018). Platelet-rich Plasma Protects HUVECs against oX-LDL-induced Injury. Open Medicine, 13, 41-52.

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Cell Viability Assessment via Annexin V-APC/7-AAD

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The culture solution containing cells was aspirated and placed in a 15 mL centrifuge tube. Centrifugation was performed at 800 rpm for 5 min. The supernatant was removed, and fresh culture medium was added. The counting started after the specimen was shaken evenly. The cells were cultured overnight in a 6-well plate at 37°C in 5% CO₂ incubator. The next day, 32 μM TAM was used. The supernatant of cell culture was collected and placed in a centrifuge tube of 3 independent experiments run in duplicate, and then centrifuged at 1500 rpm for 5 min. Later, the supernatant was removed, and the cells were washed with phosphate buffer solution (PBS) (Fuzhou Maixin Biotechnology Development Co., Ltd., China) and again centrifuged at 1500 rpm for 5 min to collect the cells. Then the specimen was washed and precipitated with binding buffer (TAKARA Biotechnology Co., Ltd., Japan), centrifuged at 1500 rpm for 5 min, and then the cells were collected. The cells were resuspended and precipitated with a 1 mL standing buffer. Then 100 μL cell suspension was added into 5 μL Annexin V-APC/7-AAD staining, and the cells were kept away from light for 10-15 min at room temperature. Finally, the samples were transferred to the flow tube (Corning company, USA) for detection.

Wu M., Ding J., Wen L., Zhou Y, & Wu W. (2021). Molecular Mechanism of Secondary Endocrine Resistance in Luminal Breast Cancer. BioMed Research International, 2021, 6618519.

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Phenotypic Characterization of Third-Generation BMSCs

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Third-generation BMSCs were collected and resuspended in complete l-DMEM, and the cell concentration was adjusted to 2.0 × 10⁷ cells/ml. Approximately 50 μl of the cell suspension was placed into the flow tube (Corning, U.S.A.), followed by 5 μl of anti-CD29/AF647, anti-CD90/PE-Cy™7, anti-CD106/PE, anti-CD45/FITC, and anti-CD11b/V450 (BD, U.S.A.). Then, 45 µl of buffer (HyClone, U.S.A.) was added to each tube. The tubes were incubated at room temperature for 30 min, washed twice with staining buffer, and then 500 µl of buffer was added to each tube for detection by flow cytometry (Beckman, U.S.A.).

Wang T., Zhang F., Peng W., Wang L., Zhang J., Dong W., Tian X., Ye C., Li Y, & Gong Y. (2022). Overexpression of NMNAT3 improves mitochondrial function and enhances antioxidative stress capacity of bone marrow mesenchymal stem cells via the NAD+-Sirt3 pathway. Bioscience Reports, 42(1), BSR20211005.

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Flow Cytometric Analysis of Murine Immune Cells

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The collected 200 μL of blood by capillary from mice was added to a flow tube (Corning, Shanghai, China) containing heparin sodium and mixed gently to prevent clotting. Antibody incubation (CD3: 1.5 μL, CD8: 1.5 μL, CD4: 0.9 μL) (Biosciences, Brisbane, CA, USA) occurred at room temperature away from light incubation for 30 min. After the antibody incubation, the whole blood was transferred to a new flow tube, 4 mL of red blood cell lysate was added, the blood was lysed for 10 min away from light, centrifuged with a horizontal rotor at 4 °C and 2000 rpm for 5 min and the supernatant was discarded and repeated. The cells were re-suspended with 300 μL of Histopaque sorting solution (Merck, Shanghai, China). An analysis was performed using a flow meter (Beckman CytoFLEX, Shanghai, China). The experiment was repeated three times.

Cao X., Huang M., Wang Y., Chen Y., Yang H, & Quan F. (2023). Immunogenicity Analysis of PCV3 Recombinant Capsid Protein Virus-like Particles and Their Application in Antibodies Detection. International Journal of Molecular Sciences, 24(12), 10377.

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