Pcmv tag3b vector

Manufactured by Agilent Technologies

Sourced in Azerbaijan

The PCMV-Tag3B vector is a plasmid DNA vector designed for the expression of recombinant proteins in mammalian cells. It contains a cytomegalovirus (CMV) promoter for high-level expression, a polylinker region for inserting the gene of interest, and a C-terminal 3xFLAG tag for detection and purification of the expressed protein.

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Lab products found in correlation

Pires2 dsred2 vector, by Takara Bio (1 mentions) Plvx ef1α ires zsgreen vector, by Takara Bio (1 mentions) Pgex 4t 2 vector, by GE Healthcare (1 mentions) Pcdna6.2 v5 dest vector, by Thermo Fisher Scientific (1 mentions) Glutathione sepharose, by GE Healthcare (1 mentions)

Close spelling variants of this product

PCMV‐Tag3B

2 protocols using pcmv tag3b vector

Subcellular Localization of Tulp Proteins

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Mouse Tulp1, Tulp2, Tulp3, and TUB cDNAs were cloned into the pIRES2-DsRed2 vector (Clontech plasmid #632420), which expresses both the gene of interest and the dsRed gene. TUB PIP₂- and IFT-A-mutant cDNAs were cloned into the pLVX-EF1α-IRES-ZsGreen vector (Clontech plasmid #631982), which expresses both the gene of interest and the ZsGreen gene. To examine the subcellular localization of TULPs in RPE1 cells, mouse Tulp1, Tulp2, Tulp3, TUB, and Tulp1^IFT-A(+) cDNAs were cloned into the pCMV-Tag3B vector (Agilent Technologies plasmid #211173), which makes it possible to visualize the expression of a gene of interest using an anti-Myc antibody.

Hong J.J., Kim K.E., Park S.Y., Bok J., Seo J.T, & Moon S.J. (2021). Differential Roles of Tubby Family Proteins in Ciliary Formation and Trafficking. Molecules and Cells, 44(8), 591-601.

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Galectin-1 and VEGF-Trap Protein Production

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Human LGALS1/Galectin-1 cDNA (GenBank No. NM_002305) was obtained from DNASU Plasmid Repository (Tempe, AZ) and subcloned into pCMV-Tag 3B vector (Agilent Technologies) with Myc tag and pGEX4T-2 vector (GE Healthcare, Piscataway, NJ) for glutathione S-transferase (GST) fusion. VEGF-Trap_R1R2 (corresponding to aflibercept) cDNA16 (link) was generated as a synthetic gene by IDT (Coralville, IA), and subcloned into the pcDNA6.2/V5-DEST vector (Life Technologies) with V5 tag. The empty pcDNA6.2/V5-DEST vector was used as a Mock transfection control. The GST-fused galectin-1 protein was expressed in an Escherichia coli strain Rosetta-gami 2 (DE3) (Novagen, Madison, WI), and purified through binding to Glutathione Sepharose (GE Healthcare). All constructs were sequence-verified before use.

Kanda A., Noda K., Saito W, & Ishida S. (2015). Aflibercept Traps Galectin-1, an Angiogenic Factor Associated with Diabetic Retinopathy. Scientific Reports, 5, 17946.

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