Anti cleaved parp 9664s

Manufactured by Cell Signaling Technology

Sourced in United States

Anti-cleaved PARP (9664S) is a laboratory reagent used in molecular biology and cell biology research. It is an antibody that specifically binds to the cleaved form of the enzyme Poly(ADP-ribose) Polymerase (PARP), a marker of apoptosis or programmed cell death. This product can be used to detect and quantify cleaved PARP in cell lysates or tissue samples using techniques such as Western blotting or immunohistochemistry.

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Lab products found in correlation

Anti brca1 sc 6954, by Santa Cruz Biotechnology (1 mentions) Anti gapdh 60004 1 1g, by Proteintech (1 mentions) Anti γh2ax 05 636, by Merck Group (1 mentions) Anti cleaved caspase 3 9664, by Cell Signaling Technology (1 mentions) Anti actin 66009 1 ig, by Proteintech (1 mentions) Anti cdh1 sc 56312, by Santa Cruz Biotechnology (1 mentions) Alexa fluor 488, by Jackson ImmunoResearch (1 mentions) Anti flag 20543 1 ap, by Proteintech (1 mentions) Anti actin, by Proteintech (1 mentions) Anti c myc sc 40, by Santa Cruz Biotechnology (1 mentions) Anti ub sc 8017, by Santa Cruz Biotechnology (1 mentions) Anti mycn sc 53993, by Santa Cruz Biotechnology (1 mentions) Anti cyclin d1 2922, by Cell Signaling Technology (1 mentions) Anti flag f3165, by Merck Group (1 mentions) Anti gapdh, by Proteintech (1 mentions)

2 protocols using anti cleaved parp 9664s

Antibody Validation for Cell Biology

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The antibodies used in this study were as follows: anti-Cdh1 (sc-56312, 1:1000 WB, Santa Cruz Biotechnology, Santa Cruz, CA, USA); anti-γH2AX (05-636, 1:500 IF, 1:1000 IF, Millipore, Billerica, MA, USA); anti-BRCA1 (sc-6954, 1:200 IF, Santa Cruz); anti-Phospho-Chk1(Ser345) (2348S, 1:1000 WB, Cell Signaling, Danvers, MA, USA); anti-cleaved Caspase-3 (9664S, 1:1000 WB, Cell Signaling); anti-cleaved PARP (9664S, 1:1000 WB, Cell Signaling); anti-Actin (66009-1-Ig, 1:5000 WB, Proteintech, Wuhan, China); anti-GAPDH (60004-1-1g, 1:5000 WB, Proteintech, Wuhan, China), and anti-PICH(8886S, 1:200 IF, Cell Signaling); the secondary antibodies conjugated to horseradish peroxidase were used for Western blotting. The secondary antibodies of anti-mouse, or anti-rabbit containg Alexa Fluor 488 or 594 were used for immunofluorescence staining (Jackson ImmunoResearch Laboratories, West Grove, PA, USA).

Li J., Lan M., Peng J., Xiong Q., Xu Y., Yang Y., Zhou Y., Liu J., Zeng Z., Yang X., Zhang Z., Zhang P., Zhu Q, & Wu W. (2022). Cdh1 Deficiency Sensitizes TNBC Cells to PARP Inhibitors. Genes, 13(5), 803.

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Antibody Validation for Western Blotting and IHC

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The antibodies used in this study were as follows: anti-USP28 (17707-1-AP, 1:1000 WB, 1:200 IHC, Proteintech); anti-MYCN (51705S, 1:1000 WB, 1:200 IHC, Cell Signaling Technology); anti-MYCN (sc-53993, 1:200 WB, Santa Cruz Biotechnology); anti-c-MYC (sc-40, 1:200 WB, Santa Cruz Biotechnology); anti-CyclinD1 (2922S, 1:1000 WB, Cell Signaling); anti-PARP (9542P, 1:1000 WB, Cell Signaling); anti-cleaved PARP (9664S, 1:1000 WB, Cell Signaling); anti-Flag (F3165 1:5000 WB, Sigma); anti-Flag (20543-1-AP, 1:2000 WB, Proteintech); anti-HA (901,503, 1:1000 WB, BioLegend); anti-Ub (SC-8017, 1:200 WB, Santa Cruz Biotechnology); anti-LSD1 (20813-1-AP, 1:1000 WB, Proteintech); anti-Ki67 (27309-1-AP, 1:1000 WB, Proteintech); anti-Actin (66009-1-Ig, 1:5000 WB, Proteintech); and anti-GAPDH (60004-1-Ig, 1:5000 WB, Proteintech); the secondary antibodies conjugated to horseradish peroxidase were used for Western blotting. The secondary antibodies of anti-mouse or anti-rabbit containing Alexa Fluor 488 or 594 were used for immunofluorescence staining (Jackson ImmunoResearch Laboratories).

Li J., Peng J., Wu L., Shen X., Zhen X., Zhang Y., Ma H., Xu Y., Xiong Q., Zhu Q, & Zhang P. (2023). The deubiquitinase USP28 maintains the expression of the transcription factor MYCN and is essential in neuroblastoma cells. The Journal of Biological Chemistry, 299(7), 104856.

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