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Nup155

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Nup155 is a core protein component of the nuclear pore complex (NPC), which is responsible for regulating the transport of molecules between the nucleus and cytoplasm. Nup155 plays a crucial role in the structural organization and function of the NPC, but a detailed description of its specific core function is not available while maintaining an unbiased and factual approach.

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Lab products found in correlation

Ab124770, by Abcam (1 mentions) Gfp ab290, by Abcam (1 mentions) Ubiquitin, by Santa Cruz Biotechnology (1 mentions) Dnmt1, by GeneTex (1 mentions) Phospho h2ax ser139, by Merck Group (1 mentions) Phospho chk2 thr68, by Cell Signaling Technology (1 mentions)

2 protocols using nup155

1

Antibody sources and properties

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Nup153 antibody (hZ) was generated by the Ullman lab; antibodies against Nup133 and Elys were a kind gift from Douglass Forbes (University of California, San Diego). Nup96 and Nup98 were provided by the Powers lab. Antibodies against the SENPs and RanBP2/Nup358 were made in the Dasso lab. Other antibodies were obtained from commercial sources as follows: GFP (Ab290; Abcam, Cambridge, MA), importin α (610485; BD Transduction, San Jose, CA), Importin β (610559; BD Transduction), Importin 7 (SC-365231; Santa Cruz Biotechnology, Dallas, TX), 414 (MMS-120P; Covance, Princeton, NJ), Nup62 (610497; BD Transduction, Irvine, CA), POM121 (GTX 102128; GeneTex), RanGAP-1 (33-0800; Zymed), Nup155 (GTX120945; GeneTex), and Sun1 (Ab124770; Abcam).
Chow K.H., Elgort S., Dasso M., Powers M.A, & Ullman K.S. (2014). The SUMO proteases SENP1 and SENP2 play a critical role in nucleoporin homeostasis and nuclear pore complex function. Molecular Biology of the Cell, 25(1), 160-168.
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2

Protein Immunoblotting and Immunofluorescence

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The following antibodies were used for immunoblotting or immunofluorescence staining: phospho-CHK2 Thr68 (Cell Signaling – 2661), DNMT1 (GeneTex – GTX116011), phospho-H2AX Ser 139 (Millipore – 05-636), KAP1 (GeneTex – GTX102226), NAP1L4 (Abcam – ab21631), NUP107 (GeneTex – GTX116664), NUP155 (GeneTex – GTX120945), NUP160 (Bethyl Labs – A301-791A), SET (Bethyl Labs – A302-261A) and ubiquitin (Santa Cruz – sc8017).
Aslanian A., Yates JR I.I.I, & Hunter T. (2014). Mass spectrometry-based quantification of the cellular response to methyl methanesulfonate treatment in human cells. DNA repair, 15, 29-38.
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