All mouse experiments were approved by the Animal Care and Use Committee at Zhengzhou University. C57BL/6 mice, Cx3cr1GFP mice (JAX # 005582), and Ai96(RCL-GCaMP6s) (JAX # 028866) mice were obtained from Jackson Labs, Ltd. Cx3cr1CreER mice were kindly provided by Dr. Steffen Jung from Weizmann Institute of Science. Cx3cr1GFP and Cx3Cr1CreER mice were maintained as heterozygous mice. Ai96 (RCL-GCaMP6s) were maintained as homozygous. Cx3Cr1CreER: RCL-GCaMP6s were obtained by crossing Cx3Cr1CreER heterozygous mice with Ai96 (RCL-GCaMP6s) homozygous mice and screening Cx3Cr1CreER heterozygous offsprings by PCR genotyping. Tamoxifen (75 mg/kg) was given intraperitoneally for 5 consecutive days at the age of 2 months to activate CreERT2 recombinase, which can excise the stop cassette in the genomic DNA of C Cx3Cr1CreER: RCL-GCaMP6s mice and thus allow the transcription of downstream GCaMP6s. LPS from Escherichia coli O127:B8 (Sigma, L5024) was injected intraperitoneally (5 µg/g body weight) and brain tissue was collected 16 h after injection. Both males and female were used and balanced numbers of sexes were mixed in our analysis and reports.
Cx3cr1gfp mice jax 005582
Manufactured by Jackson ImmunoResearch
The Cx3cr1GFP mice (JAX # 005582) are a transgenic mouse line that expresses green fluorescent protein (GFP) under the control of the Cx3cr1 promoter. Cx3cr1 is a chemokine receptor expressed on monocytes, macrophages, and dendritic cells. The GFP expression in these cells allows for their visualization and tracking in vivo.
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2 protocols using cx3cr1gfp mice jax 005582
1
In Vivo GCaMP6s Imaging of Microglia
2
Microglia-Specific Calcium Imaging in Mice
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All mouse experiments were approved by the Animal Care and Use Committee at Zhengzhou University. C57BL/6 mice, Cx3cr1 GFP mice (JAX # 005582), and Ai96(RCL-GCaMP6s) (JAX # 028866) mice were obtained from Jackson Labs, Ltd. Cx3cr1 CreER mice were kindly provided by Dr. Steffen Jung from Weizmann Institute of Science. Cx3cr1 GFP and Cx3Cr1 CreER mice were maintained as heterozygous mice.
Ai96 (RCL-GCaMP6s) were maintained as homozygous. Cx3Cr1 CreER : RCL-GCaMP6s were obtained by crossing Cx3Cr1 CreER heterozygous mice with Ai96 (RCL-GCaMP6s) homozygous mice and screening Cx3Cr1 CreER heterozygous offsprings by PCR genotyping. Tamoxifen (75 mg/kg) was given intraperitoneally for 5 consecutive days at the age of two months to activate CreERT2 recombinase, which can excise the stop cassette in the genomic DNA of Cx3Cr1CreER: RCL-GCaMP6s mice and thus allow the transcription of downstream GCaMP6s. LPS from Escherichia coli O127:B8 (Sigma, L5024) was injected intraperitoneally (5 µg/g body weight) and brain tissue was collected 16 hours after injection. Both males and female were used and balanced numbers of sexes were mixed in our analysis and reports.
Ai96 (RCL-GCaMP6s) were maintained as homozygous. Cx3Cr1 CreER : RCL-GCaMP6s were obtained by crossing Cx3Cr1 CreER heterozygous mice with Ai96 (RCL-GCaMP6s) homozygous mice and screening Cx3Cr1 CreER heterozygous offsprings by PCR genotyping. Tamoxifen (75 mg/kg) was given intraperitoneally for 5 consecutive days at the age of two months to activate CreERT2 recombinase, which can excise the stop cassette in the genomic DNA of Cx3Cr1CreER: RCL-GCaMP6s mice and thus allow the transcription of downstream GCaMP6s. LPS from Escherichia coli O127:B8 (Sigma, L5024) was injected intraperitoneally (5 µg/g body weight) and brain tissue was collected 16 hours after injection. Both males and female were used and balanced numbers of sexes were mixed in our analysis and reports.
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